TY - JOUR
T1 - Acesulfame aerobic biodegradation by enriched consortia and Chelatococcus spp.
T2 - Kinetics, transformation products, and genomic characterization
AU - Huang, Yue
AU - Deng, Yu
AU - Law, Japhet Cheuk-Fung
AU - Yang, Yu
AU - Ding, Jiahui
AU - Leung, Kelvin Sze-Yin
AU - Zhang, Tong
N1 - Funding Information:
Y.H would like to thank The University of Hong Kong for the Postgraduate Studentship and the HKU Computer Center for providing the High-Performance Computing System (HPC). Y.D wishes to thank the University of Hong Kong for the postdoctoral Fellowship. JCF.L thanks the Hong Kong Research Grants Council for financial support. Y.Y wants to thank the University of Hong Kong for the Postgraduate Studentship. All of the authors sincerely thank Miss Vicky Y.L. Fung for her technical lab support. Particularly, Y.H is grateful for the assistance and support of Mr. Zhong Yu.
Publisher Copyright:
© 2021 Elsevier Ltd. All rights reserved.
PY - 2021/9/1
Y1 - 2021/9/1
N2 - The artificial sweetener Acesulfame (ACE) has been frequently detected
in wastewater treatment plants (WWTPs) and is regarded as an emerging
pollutant due to its low biodegradability. However, recent observations
of ACE biodegradation in WWTPs have stimulated interest in the
ACE-degrading bacteria and mineralization pathways. In this study,
next-generation sequencing methods, Illumina and Nanopore sequencing,
were combined to explore the ACE-degrading communities enriched from the
activated sludge of six municipal wastewater treatment plants.
Metagenomic investigations indicated that all enrichments were similarly
dominated by the phyla Proteobacteria and Planctomycetes.
Notably, at the species level, four metagenome-assembled genomes (MAGs)
were shared by six enriched communities with considerable abundances,
indicating that they may be responsible for ACE biodegradation in the
enrichments. Besides, two ACE-degrading pure strains, affiliated to the
genus Chelatococcus, were isolated from the enrichment. The
genomic analysis showed that these two isolates were the new species
that were genetically distinct from their relatives. Two type strains, Chelatococcus asaccharovorans DSM 6462 and Chelatococcus composti
DSM 101465, could not degrade ACE, implying that the ACE-degrading
capability was not shared among the different species in the genus Chelatococcus.
The results of the degradation experiment showed that the two isolates
could use ACE as the sole carbon source and mineralize ~90% of the total
organic carbon. Three biotransformation products (TP96, TP180B, and
TP182B) were demonstrated by UPLC-QTOF-MS. The results of this study
provide valuable insights into ACE biodegradation and its
biotransformation products.
AB - The artificial sweetener Acesulfame (ACE) has been frequently detected
in wastewater treatment plants (WWTPs) and is regarded as an emerging
pollutant due to its low biodegradability. However, recent observations
of ACE biodegradation in WWTPs have stimulated interest in the
ACE-degrading bacteria and mineralization pathways. In this study,
next-generation sequencing methods, Illumina and Nanopore sequencing,
were combined to explore the ACE-degrading communities enriched from the
activated sludge of six municipal wastewater treatment plants.
Metagenomic investigations indicated that all enrichments were similarly
dominated by the phyla Proteobacteria and Planctomycetes.
Notably, at the species level, four metagenome-assembled genomes (MAGs)
were shared by six enriched communities with considerable abundances,
indicating that they may be responsible for ACE biodegradation in the
enrichments. Besides, two ACE-degrading pure strains, affiliated to the
genus Chelatococcus, were isolated from the enrichment. The
genomic analysis showed that these two isolates were the new species
that were genetically distinct from their relatives. Two type strains, Chelatococcus asaccharovorans DSM 6462 and Chelatococcus composti
DSM 101465, could not degrade ACE, implying that the ACE-degrading
capability was not shared among the different species in the genus Chelatococcus.
The results of the degradation experiment showed that the two isolates
could use ACE as the sole carbon source and mineralize ~90% of the total
organic carbon. Three biotransformation products (TP96, TP180B, and
TP182B) were demonstrated by UPLC-QTOF-MS. The results of this study
provide valuable insights into ACE biodegradation and its
biotransformation products.
KW - Acesulfame
KW - Biodegradation
KW - Chelatococcus
KW - Genome-centric
KW - Transformation products
UR - http://www.scopus.com/inward/record.url?scp=85111253261&partnerID=8YFLogxK
U2 - 10.1016/j.watres.2021.117454
DO - 10.1016/j.watres.2021.117454
M3 - Journal article
C2 - 34332189
AN - SCOPUS:85111253261
SN - 0043-1354
VL - 202
JO - Water Research
JF - Water Research
M1 - 117454
ER -