TY - JOUR
T1 - Accumulation, distribution and transformation of DDT and PCBs by Phragmites australis and Oryza sativa L.
T2 - II. Enzyme study
AU - Chu, W. K.
AU - Wong, M. H.
AU - Zhang, J.
N1 - Funding Information:
This research was supported by the Central Allocation Group Research, University Grants Committee, Hong Kong (Code No: HKBU-2/00C).
PY - 2006/2
Y1 - 2006/2
N2 - Two wetland plant species, Phragmites australis and Oryza sativa, were grown in a glasshouse under hydroponics conditions. Enzyme extracts from different parts of the plants were used to determine the transformation rate of o,p′-DDT, p,p′-DDT and PCBs. The organic pollutants were directly spiked into the enzyme extracts, and samples were collected every 30 min and analyzed with a GC-ECD. Root extracts of P. australis readily degraded and transformed DDT and some PCB congeners with a low degree of chlorination. In contrast, crude extracts of O. sativa showed no appreciable degradation or transformation of DDT or PCBs. Inhibition studies indicated that the degradation and transformation of both DDT and PCBs by P. australis enzymes were partly mediated by peroxidase and the plant P-450 system. PCBs with a high degree of chlorination were highly resistant to transformation or degradation by plant enzymes. Both wetland plant species accumulated substantial quantities of the persistent organic chemicals but had different degradation capacities. The enzyme systems in P. australis were much more effective that those in rice in the degradation and transformation of the organic pollutants.
AB - Two wetland plant species, Phragmites australis and Oryza sativa, were grown in a glasshouse under hydroponics conditions. Enzyme extracts from different parts of the plants were used to determine the transformation rate of o,p′-DDT, p,p′-DDT and PCBs. The organic pollutants were directly spiked into the enzyme extracts, and samples were collected every 30 min and analyzed with a GC-ECD. Root extracts of P. australis readily degraded and transformed DDT and some PCB congeners with a low degree of chlorination. In contrast, crude extracts of O. sativa showed no appreciable degradation or transformation of DDT or PCBs. Inhibition studies indicated that the degradation and transformation of both DDT and PCBs by P. australis enzymes were partly mediated by peroxidase and the plant P-450 system. PCBs with a high degree of chlorination were highly resistant to transformation or degradation by plant enzymes. Both wetland plant species accumulated substantial quantities of the persistent organic chemicals but had different degradation capacities. The enzyme systems in P. australis were much more effective that those in rice in the degradation and transformation of the organic pollutants.
KW - DDT
KW - Oryza sativa
KW - PCBs
KW - Persistent organic chemicals
KW - Phragmites australis
KW - Phytoremediation
UR - http://www.scopus.com/inward/record.url?scp=33745840832&partnerID=8YFLogxK
U2 - 10.1007/s10653-005-9028-7
DO - 10.1007/s10653-005-9028-7
M3 - Journal article
C2 - 16547764
AN - SCOPUS:33745840832
SN - 0269-4042
VL - 28
SP - 169
EP - 181
JO - Environmental Geochemistry and Health
JF - Environmental Geochemistry and Health
IS - 1-2
ER -