Accelerated direct electrochemistry of hemoglobin based on hemoglobin-carbon nanotube (Hb-CNT) assembly

Renyun Zhang, Xuemei Wang*, Kwok Keung SHIU

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

39 Citations (Scopus)


In this study, we have demonstrated that hemoglobin can be coupled to acid-treated multiwall carbon nanotubes in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and assembled as hemoglobin-carbon nanotube (Hb-CNT) composites. Our observations of the electrochemical studies demonstrate that the electrochemical response of Hb-CNT assembled in the presence of EDC is much higher than that in the absence of EDC. It is evident that the direct electron transfer of hemoglobin could be effectively accelerated in the Hb-CNT assembly by using EDC on a glassy carbon electrode (GCE), and the relative electron transfer rate constant Ks is found to be 1.02 ± 0.05   s-1. The results of our studies illustrate that the assembly of hemoglobin-multiwall carbon nanotubes using EDC could provide a novel strategy to effectively facilitate the direct electrochemistry of heme-containing proteins, which could be further utilized as a promising biosensor for some specific biological substrate and related biological process.

Original languageEnglish
Pages (from-to)517-522
Number of pages6
JournalJournal of Colloid and Interface Science
Issue number2
Publication statusPublished - 15 Dec 2007

Scopus Subject Areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Surfaces, Coatings and Films
  • Colloid and Surface Chemistry

User-Defined Keywords

  • Carbon nanotubes
  • Chemically modified electrode
  • Electrochemistry
  • Hemoglobin
  • Nanotechnology


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