Accelerated direct electrochemistry of hemoglobin based on hemoglobin-carbon nanotube (Hb-CNT) assembly

Renyun Zhang; Xuemei Wang; Kwok Keung Shiu

doi:10.1016/j.jcis.2007.08.016

Accelerated direct electrochemistry of hemoglobin based on hemoglobin-carbon nanotube (Hb-CNT) assembly

Renyun Zhang, Xuemei Wang^*, Kwok Keung Shiu

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

40 Citations (Scopus)

Abstract

In this study, we have demonstrated that hemoglobin can be coupled to acid-treated multiwall carbon nanotubes in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and assembled as hemoglobin-carbon nanotube (Hb-CNT) composites. Our observations of the electrochemical studies demonstrate that the electrochemical response of Hb-CNT assembled in the presence of EDC is much higher than that in the absence of EDC. It is evident that the direct electron transfer of hemoglobin could be effectively accelerated in the Hb-CNT assembly by using EDC on a glassy carbon electrode (GCE), and the relative electron transfer rate constant K_s is found to be 1.02 ± 0.05 s^-1. The results of our studies illustrate that the assembly of hemoglobin-multiwall carbon nanotubes using EDC could provide a novel strategy to effectively facilitate the direct electrochemistry of heme-containing proteins, which could be further utilized as a promising biosensor for some specific biological substrate and related biological process.

Original language	English
Pages (from-to)	517-522
Number of pages	6
Journal	Journal of Colloid and Interface Science
Volume	316
Issue number	2
DOIs	https://doi.org/10.1016/j.jcis.2007.08.016
Publication status	Published - 15 Dec 2007

Scopus Subject Areas

Electronic, Optical and Magnetic Materials
Biomaterials
Surfaces, Coatings and Films
Colloid and Surface Chemistry

User-Defined Keywords

Carbon nanotubes
Chemically modified electrode
Electrochemistry
Hemoglobin
Nanotechnology

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10.1016/j.jcis.2007.08.016

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title = "Accelerated direct electrochemistry of hemoglobin based on hemoglobin-carbon nanotube (Hb-CNT) assembly",

abstract = "In this study, we have demonstrated that hemoglobin can be coupled to acid-treated multiwall carbon nanotubes in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and assembled as hemoglobin-carbon nanotube (Hb-CNT) composites. Our observations of the electrochemical studies demonstrate that the electrochemical response of Hb-CNT assembled in the presence of EDC is much higher than that in the absence of EDC. It is evident that the direct electron transfer of hemoglobin could be effectively accelerated in the Hb-CNT assembly by using EDC on a glassy carbon electrode (GCE), and the relative electron transfer rate constant Ks is found to be 1.02 ± 0.05 s-1. The results of our studies illustrate that the assembly of hemoglobin-multiwall carbon nanotubes using EDC could provide a novel strategy to effectively facilitate the direct electrochemistry of heme-containing proteins, which could be further utilized as a promising biosensor for some specific biological substrate and related biological process.",

keywords = "Carbon nanotubes, Chemically modified electrode, Electrochemistry, Hemoglobin, Nanotechnology",

author = "Renyun Zhang and Xuemei Wang and Shiu, {Kwok Keung}",

note = "Funding Information: The National Natural Science Foundation of China (20675014, 20535010, and 60121101) and the program for New Century Excellent Talents in University, the Chinese Ministry of Education (050462) are greatly appreciated. Dr. Zhang especially thanks the Foundation for Excellent PhD students and the Innovation Program of Jiangsu Province for their support. ",

year = "2007",

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doi = "10.1016/j.jcis.2007.08.016",

language = "English",

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journal = "Journal of Colloid and Interface Science",

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AU - Zhang, Renyun

AU - Wang, Xuemei

AU - Shiu, Kwok Keung

N1 - Funding Information: The National Natural Science Foundation of China (20675014, 20535010, and 60121101) and the program for New Century Excellent Talents in University, the Chinese Ministry of Education (050462) are greatly appreciated. Dr. Zhang especially thanks the Foundation for Excellent PhD students and the Innovation Program of Jiangsu Province for their support.

PY - 2007/12/15

Y1 - 2007/12/15

N2 - In this study, we have demonstrated that hemoglobin can be coupled to acid-treated multiwall carbon nanotubes in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and assembled as hemoglobin-carbon nanotube (Hb-CNT) composites. Our observations of the electrochemical studies demonstrate that the electrochemical response of Hb-CNT assembled in the presence of EDC is much higher than that in the absence of EDC. It is evident that the direct electron transfer of hemoglobin could be effectively accelerated in the Hb-CNT assembly by using EDC on a glassy carbon electrode (GCE), and the relative electron transfer rate constant Ks is found to be 1.02 ± 0.05 s-1. The results of our studies illustrate that the assembly of hemoglobin-multiwall carbon nanotubes using EDC could provide a novel strategy to effectively facilitate the direct electrochemistry of heme-containing proteins, which could be further utilized as a promising biosensor for some specific biological substrate and related biological process.

AB - In this study, we have demonstrated that hemoglobin can be coupled to acid-treated multiwall carbon nanotubes in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and assembled as hemoglobin-carbon nanotube (Hb-CNT) composites. Our observations of the electrochemical studies demonstrate that the electrochemical response of Hb-CNT assembled in the presence of EDC is much higher than that in the absence of EDC. It is evident that the direct electron transfer of hemoglobin could be effectively accelerated in the Hb-CNT assembly by using EDC on a glassy carbon electrode (GCE), and the relative electron transfer rate constant Ks is found to be 1.02 ± 0.05 s-1. The results of our studies illustrate that the assembly of hemoglobin-multiwall carbon nanotubes using EDC could provide a novel strategy to effectively facilitate the direct electrochemistry of heme-containing proteins, which could be further utilized as a promising biosensor for some specific biological substrate and related biological process.

KW - Carbon nanotubes

KW - Chemically modified electrode

KW - Electrochemistry

KW - Hemoglobin

KW - Nanotechnology

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DO - 10.1016/j.jcis.2007.08.016

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JF - Journal of Colloid and Interface Science

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Accelerated direct electrochemistry of hemoglobin based on hemoglobin-carbon nanotube (Hb-CNT) assembly

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