Abscisic acid is required for root elongation associated with Ca2+ influx in response to water stress

Wei Yuan; Qian Zhang; Ying Li; Qianwen Wang; Feiyun Xu; Xiaolin Dang; Weifeng Xu; Jianhua Zhang; Rui Miao

doi:10.1016/j.plaphy.2021.11.002

Abscisic acid is required for root elongation associated with Ca²⁺ influx in response to water stress

Wei Yuan, Qian Zhang, Ying Li, Qianwen Wang, Feiyun Xu, Xiaolin Dang, Weifeng Xu^*, Jianhua Zhang, Rui Miao^*

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

9 Citations (Scopus)

Abstract

Abscisic acid (ABA) is a critical hormone for plant survival upon water stress. In this study, a large-scale mutants of Arabidopsis ecotype Columbia-0 (Col-0) by ethyl methanesulfonate (EMS)-mutagenesis were generated, and an improved root elongation under water-stress 1 (irew1) mutant showing significantly enhanced root growth was isolated upon a water potential gradient assay. Then, irew1 and ABA-related mutants in Arabidopsis or tomato plants were observed under water potential gradient assay or water-deficient condition. ABA pathway, Ca²⁺ response and primary root (PR) elongation rate were monitored in addition to DNA- and RNA-Seq analyses. We found that based on phenotyping and transcriptional analyses, irew1 exhibited the enhanced PR growth, ABA and Ca²⁺ responses compared to wild-type subjected to water stress. Interestingly, exogenous Ca²⁺ application enhanced PR growth of irew1, ABA-biosynthesis deficient mutants in Arabidopsis and tomato plants in response to water potential gradients or water-deficient condition. In combination with other ABA-related mutants and pharmacological study, our results suggest that ABA is required for root elongation associated with Ca²⁺ influx in response to water stress.

Original language	English
Pages (from-to)	127-137
Number of pages	11
Journal	Plant Physiology and Biochemistry
Volume	169
Early online date	10 Nov 2021
DOIs	https://doi.org/10.1016/j.plaphy.2021.11.002
Publication status	Published - Dec 2021

Scopus Subject Areas

Physiology
Genetics
Plant Science

User-Defined Keywords

Abscisic acid
Arabidopsis thaliana
Calcium
Root
Tomato
Water stress

Access to Document

10.1016/j.plaphy.2021.11.002

Cite this

@article{6c6368c8c6514470a64c50ea4081819e,

title = "Abscisic acid is required for root elongation associated with Ca2+ influx in response to water stress",

abstract = "Abscisic acid (ABA) is a critical hormone for plant survival upon water stress. In this study, a large-scale mutants of Arabidopsis ecotype Columbia-0 (Col-0) by ethyl methanesulfonate (EMS)-mutagenesis were generated, and an improved root elongation under water-stress 1 (irew1) mutant showing significantly enhanced root growth was isolated upon a water potential gradient assay. Then, irew1 and ABA-related mutants in Arabidopsis or tomato plants were observed under water potential gradient assay or water-deficient condition. ABA pathway, Ca2+ response and primary root (PR) elongation rate were monitored in addition to DNA- and RNA-Seq analyses. We found that based on phenotyping and transcriptional analyses, irew1 exhibited the enhanced PR growth, ABA and Ca2+ responses compared to wild-type subjected to water stress. Interestingly, exogenous Ca2+ application enhanced PR growth of irew1, ABA-biosynthesis deficient mutants in Arabidopsis and tomato plants in response to water potential gradients or water-deficient condition. In combination with other ABA-related mutants and pharmacological study, our results suggest that ABA is required for root elongation associated with Ca2+ influx in response to water stress.",

keywords = "Abscisic acid, Arabidopsis thaliana, Calcium, Root, Tomato, Water stress",

author = "Wei Yuan and Qian Zhang and Ying Li and Qianwen Wang and Feiyun Xu and Xiaolin Dang and Weifeng Xu and Jianhua Zhang and Rui Miao",

note = "Funding Information: We are grateful for grant support from the National Key R&D Program of China (2017YFE0118100 and 2018YFD02003025), National Natural Science Foundation of China (Nos. 31761130073, 31872169, 31600209), a Newton Advanced Fellowship (NSFC-RS: NA160430), Fujian Province Education Department Funding (JK2017015) and Research Grant of FAFU (KXGH17005). We thank Dr. Ian Dodd (Lancaster University, UK) and Dr. Hai-Dong Ding (Yangzhou University, China) for seeds of Solanum lycopersicum L. cv Lukullus (Lu) and abscisic acid (ABA)-biosynthesis deficient mutant notabilis (not) with background of Lu. Publisher Copyright: {\textcopyright} 2021 Published by Elsevier Masson SAS. All rights reserved.",

year = "2021",

month = dec,

doi = "10.1016/j.plaphy.2021.11.002",

language = "English",

volume = "169",

pages = "127--137",

journal = "Plant Physiology and Biochemistry",

issn = "0981-9428",

publisher = "Elsevier Masson SAS",

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TY - JOUR

T1 - Abscisic acid is required for root elongation associated with Ca2+ influx in response to water stress

AU - Yuan, Wei

AU - Zhang, Qian

AU - Li, Ying

AU - Wang, Qianwen

AU - Xu, Feiyun

AU - Dang, Xiaolin

AU - Xu, Weifeng

AU - Zhang, Jianhua

AU - Miao, Rui

N1 - Funding Information: We are grateful for grant support from the National Key R&D Program of China (2017YFE0118100 and 2018YFD02003025), National Natural Science Foundation of China (Nos. 31761130073, 31872169, 31600209), a Newton Advanced Fellowship (NSFC-RS: NA160430), Fujian Province Education Department Funding (JK2017015) and Research Grant of FAFU (KXGH17005). We thank Dr. Ian Dodd (Lancaster University, UK) and Dr. Hai-Dong Ding (Yangzhou University, China) for seeds of Solanum lycopersicum L. cv Lukullus (Lu) and abscisic acid (ABA)-biosynthesis deficient mutant notabilis (not) with background of Lu. Publisher Copyright: © 2021 Published by Elsevier Masson SAS. All rights reserved.

PY - 2021/12

Y1 - 2021/12

N2 - Abscisic acid (ABA) is a critical hormone for plant survival upon water stress. In this study, a large-scale mutants of Arabidopsis ecotype Columbia-0 (Col-0) by ethyl methanesulfonate (EMS)-mutagenesis were generated, and an improved root elongation under water-stress 1 (irew1) mutant showing significantly enhanced root growth was isolated upon a water potential gradient assay. Then, irew1 and ABA-related mutants in Arabidopsis or tomato plants were observed under water potential gradient assay or water-deficient condition. ABA pathway, Ca2+ response and primary root (PR) elongation rate were monitored in addition to DNA- and RNA-Seq analyses. We found that based on phenotyping and transcriptional analyses, irew1 exhibited the enhanced PR growth, ABA and Ca2+ responses compared to wild-type subjected to water stress. Interestingly, exogenous Ca2+ application enhanced PR growth of irew1, ABA-biosynthesis deficient mutants in Arabidopsis and tomato plants in response to water potential gradients or water-deficient condition. In combination with other ABA-related mutants and pharmacological study, our results suggest that ABA is required for root elongation associated with Ca2+ influx in response to water stress.

AB - Abscisic acid (ABA) is a critical hormone for plant survival upon water stress. In this study, a large-scale mutants of Arabidopsis ecotype Columbia-0 (Col-0) by ethyl methanesulfonate (EMS)-mutagenesis were generated, and an improved root elongation under water-stress 1 (irew1) mutant showing significantly enhanced root growth was isolated upon a water potential gradient assay. Then, irew1 and ABA-related mutants in Arabidopsis or tomato plants were observed under water potential gradient assay or water-deficient condition. ABA pathway, Ca2+ response and primary root (PR) elongation rate were monitored in addition to DNA- and RNA-Seq analyses. We found that based on phenotyping and transcriptional analyses, irew1 exhibited the enhanced PR growth, ABA and Ca2+ responses compared to wild-type subjected to water stress. Interestingly, exogenous Ca2+ application enhanced PR growth of irew1, ABA-biosynthesis deficient mutants in Arabidopsis and tomato plants in response to water potential gradients or water-deficient condition. In combination with other ABA-related mutants and pharmacological study, our results suggest that ABA is required for root elongation associated with Ca2+ influx in response to water stress.

KW - Abscisic acid

KW - Arabidopsis thaliana

KW - Calcium

KW - Root

KW - Tomato

KW - Water stress

UR - http://www.scopus.com/inward/record.url?scp=85118832434&partnerID=8YFLogxK

U2 - 10.1016/j.plaphy.2021.11.002

DO - 10.1016/j.plaphy.2021.11.002

M3 - Journal article

C2 - 34781213

AN - SCOPUS:85118832434

SN - 0981-9428

VL - 169

SP - 127

EP - 137

JO - Plant Physiology and Biochemistry

JF - Plant Physiology and Biochemistry

ER -