TY - JOUR
T1 - Abscisic Acid Is Required for Root Elongation Associated With Ca2+ Influx in Response to Water Stress
AU - Yuan, Wei
AU - Zhang, Qian
AU - Li, Ying
AU - Wang, Qianwen
AU - Xu, Feiyun
AU - Dang, Xiaolin
AU - Xu, Weifeng
AU - Zhang, Jianhua
AU - Miao, Rui
N1 - Funding Information:
We are grateful for grant support from the National Key R&D Program of China (2017YFE0118100 and 2018YFD02003025), National Natural Science Foundation of China (Nos. 31761130073, 31872169, and 31600209), a Newton Advanced Fellowship (NSFC-RS: NA160430), Fujian Province Education Department Funding (JK2017015) and Research Grant of FAFU (KXGH17005).
Funding Information:
We thank Dr. Ian Dodd (Lancaster University, United Kingdom) and Dr.Hai-Dong Ding (Yangzhou University, China) for seeds of Solanum lycopersicum L. cv Lukullus (Lu) and abscisic acid (ABA)-biosynthesis deficient mutant notabilis (not) with background of Lu. Funding. We are grateful for grant support from the National Key R&D Program of China (2017YFE0118100 and 2018YFD02003025), National Natural Science Foundation of China (Nos. 31761130073, 31872169, and 31600209), a Newton Advanced Fellowship (NSFC-RS: NA160430), Fujian Province Education Department Funding (JK2017015) and Research Grant of FAFU (KXGH17005).
PY - 2020/4/22
Y1 - 2020/4/22
N2 - Abscisic acid (ABA) is a critical hormone for plant survival under water stress. In this study, large-scale mutants of the Arabidopsis ecotype Columbia-0 (Col-0) were generated by ethyl methanesulfonate (EMS)-mutagenesis, and an improved root elongation under water-stress 1 (irew1) mutant showing significantly enhanced root growth was isolated under a water potential gradient assay. Then, irew1 and ABA-related mutants in Arabidopsis or tomato plants were observed under water potential gradient assay or water-deficient conditions. ABA pathway, Ca2+ response, and primary root (PR) elongation rate were monitored in addition to DNA- and RNA-Seq analyses. We found that based on phenotyping and transcriptional analyses, irew1 exhibited enhanced PR growth, ABA, and Ca2+ responses, compared to wild type subjected to water stress. Interestingly, exogenous Ca2+ application enhanced PR growth of irew1, ABA-biosynthesis deficient mutants in Arabidopsis, and tomato plants, in response to water potential gradients or water-deficient conditions. In combination with other ABA-related mutants and pharmacological studies, our results suggest that ABA is required for root elongation associated with Ca2+ influx in response to water stress.
AB - Abscisic acid (ABA) is a critical hormone for plant survival under water stress. In this study, large-scale mutants of the Arabidopsis ecotype Columbia-0 (Col-0) were generated by ethyl methanesulfonate (EMS)-mutagenesis, and an improved root elongation under water-stress 1 (irew1) mutant showing significantly enhanced root growth was isolated under a water potential gradient assay. Then, irew1 and ABA-related mutants in Arabidopsis or tomato plants were observed under water potential gradient assay or water-deficient conditions. ABA pathway, Ca2+ response, and primary root (PR) elongation rate were monitored in addition to DNA- and RNA-Seq analyses. We found that based on phenotyping and transcriptional analyses, irew1 exhibited enhanced PR growth, ABA, and Ca2+ responses, compared to wild type subjected to water stress. Interestingly, exogenous Ca2+ application enhanced PR growth of irew1, ABA-biosynthesis deficient mutants in Arabidopsis, and tomato plants, in response to water potential gradients or water-deficient conditions. In combination with other ABA-related mutants and pharmacological studies, our results suggest that ABA is required for root elongation associated with Ca2+ influx in response to water stress.
KW - abscisic acid
KW - Arabidopsis thaliana
KW - calcium
KW - root
KW - tomato
KW - water stress
UR - http://www.scopus.com/inward/record.url?scp=85084252477&partnerID=8YFLogxK
U2 - 10.3389/fpls.2020.00332
DO - 10.3389/fpls.2020.00332
M3 - Journal article
AN - SCOPUS:85084252477
SN - 1664-462X
VL - 11
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
M1 - 332
ER -