TY - JOUR
T1 - A Zero Cross-Talk Ratiometric Two-Photon Probe for Imaging of Acid pH in Living Cells and Tissues and Early Detection of Tumor in Mouse Model
AU - Xu, Di
AU - Li, Yinhui
AU - Poon, Chung Yan
AU - Chan, Hei Nga
AU - Li, Hung Wing
AU - Wong, Man Shing
N1 - Funding Information:
This work was supported by Hong Kong Research Grant Council (GRF 12301317), the National Natural Science Foundation of China (No. 21675135), the Faculty Research Grant of Hong Kong Baptist University (FRG2/17-18/057), 2014 Hong Kong Scholars Program, and Institute of Molecular Functional Materials, which was supported by a grant from the University Grants Committee, Areas of Excellence Scheme (AoE/P-03/08).
Publisher copyright:
© 2018 American Chemical Society
PY - 2018/8/7
Y1 - 2018/8/7
N2 - Acid-base disorders disrupt proper cellular functions, which are associated with diverse diseases. Development of highly sensitive pH probes being capable of detecting and monitoring the minor changes of pH environment in living systems is of considerable interest to diagnose disease as well as investigate biochemical processes in vivo. We report herein two novel high-resolution ratiometric two-photon (TP) fluorescent probes, namely, PSIOH and PSIBOH derived from carbazole-oxazolidine π-conjugated system for effective sensing and monitoring acid pH in a biological system. Remarkably, PSIOH exhibited the largest emission shift of ∼169 nm from 435 to 604 nm upon pH changing from basic to acidic with an ideal pKa value of 6.6 within a linear pH variation range of 6.2-7.0, which is highly desirable for high-resolution tracking and imaging the minor fluctuation of pH in live cells and tissues. PSIOH also exhibits high pH sensitivity, excellent photostability, and reversibility as well as low cytotoxicity. More importantly, this probe was successfully applied to (i) sense and visualize the pH alteration in HeLa cells caused by various types of exogenous stimulation and (ii) detect and differentiate cancer and tumors in liver tissues and a mouse model, realizing its practical in vitro and in vivo applications.
AB - Acid-base disorders disrupt proper cellular functions, which are associated with diverse diseases. Development of highly sensitive pH probes being capable of detecting and monitoring the minor changes of pH environment in living systems is of considerable interest to diagnose disease as well as investigate biochemical processes in vivo. We report herein two novel high-resolution ratiometric two-photon (TP) fluorescent probes, namely, PSIOH and PSIBOH derived from carbazole-oxazolidine π-conjugated system for effective sensing and monitoring acid pH in a biological system. Remarkably, PSIOH exhibited the largest emission shift of ∼169 nm from 435 to 604 nm upon pH changing from basic to acidic with an ideal pKa value of 6.6 within a linear pH variation range of 6.2-7.0, which is highly desirable for high-resolution tracking and imaging the minor fluctuation of pH in live cells and tissues. PSIOH also exhibits high pH sensitivity, excellent photostability, and reversibility as well as low cytotoxicity. More importantly, this probe was successfully applied to (i) sense and visualize the pH alteration in HeLa cells caused by various types of exogenous stimulation and (ii) detect and differentiate cancer and tumors in liver tissues and a mouse model, realizing its practical in vitro and in vivo applications.
UR - http://www.scopus.com/inward/record.url?scp=85049609275&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.8b00520
DO - 10.1021/acs.analchem.8b00520
M3 - Journal article
C2 - 29961313
AN - SCOPUS:85049609275
SN - 0003-2700
VL - 90
SP - 8800
EP - 8806
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 15
ER -