A versatile nanomachine for the sensitive detection of platelet-derived growth factor-BB utilizing a G-quadruplex-selective iridium(III) complex

Lihua Lu; Zhifeng Mao; Tian Shu Kang; Chung Hang Leung; Edmond Dik Lung Ma

doi:10.1016/j.bios.2016.05.026

A versatile nanomachine for the sensitive detection of platelet-derived growth factor-BB utilizing a G-quadruplex-selective iridium(III) complex

Lihua Lu^*, Zhifeng Mao, Tian Shu Kang, Chung Hang Leung^*, Edmond Dik Lung Ma^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

39 Citations (Scopus)

Abstract

The novel iridium(III) complex 1 was found to be highly selective for G-quadruplex DNA, and was employed for the development of a versatile nanomachine. In the nanomachine, the single-stranded DNA (ssDNA) oligonucleotide ON1 hybridizes with the hairpin DNA oligonucleotide ON2, leading to the formation of a 5'-recessed double-stranded DNA (dsDNA) structure and the simultaneous release of a G-rich DNA sequence, which folds into a G-quadruplex motif that is recognized by complex 1. Upon the addition of Exo III, the dsDNA substrate is digested leading to the liberation of ON1 ssDNA, which enters a new cycle of the nanomachine. This nanomachine was successfully used to detect PDGF-BB by combination with a simple pre-procedure module, and exhibited a linear response between luminescence intensity and PDGF-BB concentration in the range of 10 to 300 pM (R² = 0.992), with a limit of detection for PDGF-BB of 10 pM. This assay was highly selective for PDGF-BB over other proteins, and exhibited potential use in biological sample analysis. Moreover, the versatility of the nanomachine was demonstrated by adapting the nanomachine for the detection of thrombin by simply changing the pre-procedure module.

Original language	English
Pages (from-to)	300-309
Number of pages	10
Journal	Biosensors and Bioelectronics
Volume	85
DOIs	https://doi.org/10.1016/j.bios.2016.05.026
Publication status	Published - 15 Nov 2016

Scopus Subject Areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

User-Defined Keywords

G-quadruplex
Iridium(III) complex
Luminescent
Nanomachine
PDGF-BB

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10.1016/j.bios.2016.05.026

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@article{46f2308d49504194bd8ca718b0935f95,

title = "A versatile nanomachine for the sensitive detection of platelet-derived growth factor-BB utilizing a G-quadruplex-selective iridium(III) complex",

abstract = "The novel iridium(III) complex 1 was found to be highly selective for G-quadruplex DNA, and was employed for the development of a versatile nanomachine. In the nanomachine, the single-stranded DNA (ssDNA) oligonucleotide ON1 hybridizes with the hairpin DNA oligonucleotide ON2, leading to the formation of a 5'-recessed double-stranded DNA (dsDNA) structure and the simultaneous release of a G-rich DNA sequence, which folds into a G-quadruplex motif that is recognized by complex 1. Upon the addition of Exo III, the dsDNA substrate is digested leading to the liberation of ON1 ssDNA, which enters a new cycle of the nanomachine. This nanomachine was successfully used to detect PDGF-BB by combination with a simple pre-procedure module, and exhibited a linear response between luminescence intensity and PDGF-BB concentration in the range of 10 to 300 pM (R2 = 0.992), with a limit of detection for PDGF-BB of 10 pM. This assay was highly selective for PDGF-BB over other proteins, and exhibited potential use in biological sample analysis. Moreover, the versatility of the nanomachine was demonstrated by adapting the nanomachine for the detection of thrombin by simply changing the pre-procedure module.",

keywords = "G-quadruplex, Iridium(III) complex, Luminescent, Nanomachine, PDGF-BB",

author = "Lihua Lu and Zhifeng Mao and Kang, {Tian Shu} and Leung, {Chung Hang} and Ma, {Edmond Dik Lung}",

note = "Funding Information: This work is supported by Hong Kong Baptist University ( FRG2/14-15/004 and FRG2/15-16/002 ), the Health and Medical Research Fund ( HMRF/14130522 ), the Research Grants Council ( HKBU/201811 , HKBU/204612 and HKBU/201913 ), the French Agence Nationale de la Recherche/Research Grants Council Joint Research Scheme (AHKBU201/12; Oligoswitch ANR-12-IS07-0001) ( A-HKBU201/12 ), National Natural Science Foundation of China ( 21575121 ), Guangdong Province Natural Science Foundation ( 2015A030313816 ), Hong Kong Baptist University Century Club Sponsorship Scheme 2015, Interdisciplinary Research Matching Scheme ( RC-IRMS/14-15/06 ), the Science and Technology Development Fund , Macau SAR ( 098/2014/A2 ), the University of Macau ( MYRG091(Y3-L2)-ICMS12-LCH , MYRG2015-00137-ICMS-QRCM MRG044/LCH/2015/ICMS and MRG023/LCH/2013/ICMS ). ",

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doi = "10.1016/j.bios.2016.05.026",

language = "English",

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AU - Lu, Lihua

AU - Mao, Zhifeng

AU - Kang, Tian Shu

AU - Leung, Chung Hang

AU - Ma, Edmond Dik Lung

N1 - Funding Information: This work is supported by Hong Kong Baptist University ( FRG2/14-15/004 and FRG2/15-16/002 ), the Health and Medical Research Fund ( HMRF/14130522 ), the Research Grants Council ( HKBU/201811 , HKBU/204612 and HKBU/201913 ), the French Agence Nationale de la Recherche/Research Grants Council Joint Research Scheme (AHKBU201/12; Oligoswitch ANR-12-IS07-0001) ( A-HKBU201/12 ), National Natural Science Foundation of China ( 21575121 ), Guangdong Province Natural Science Foundation ( 2015A030313816 ), Hong Kong Baptist University Century Club Sponsorship Scheme 2015, Interdisciplinary Research Matching Scheme ( RC-IRMS/14-15/06 ), the Science and Technology Development Fund , Macau SAR ( 098/2014/A2 ), the University of Macau ( MYRG091(Y3-L2)-ICMS12-LCH , MYRG2015-00137-ICMS-QRCM MRG044/LCH/2015/ICMS and MRG023/LCH/2013/ICMS ).

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N2 - The novel iridium(III) complex 1 was found to be highly selective for G-quadruplex DNA, and was employed for the development of a versatile nanomachine. In the nanomachine, the single-stranded DNA (ssDNA) oligonucleotide ON1 hybridizes with the hairpin DNA oligonucleotide ON2, leading to the formation of a 5'-recessed double-stranded DNA (dsDNA) structure and the simultaneous release of a G-rich DNA sequence, which folds into a G-quadruplex motif that is recognized by complex 1. Upon the addition of Exo III, the dsDNA substrate is digested leading to the liberation of ON1 ssDNA, which enters a new cycle of the nanomachine. This nanomachine was successfully used to detect PDGF-BB by combination with a simple pre-procedure module, and exhibited a linear response between luminescence intensity and PDGF-BB concentration in the range of 10 to 300 pM (R2 = 0.992), with a limit of detection for PDGF-BB of 10 pM. This assay was highly selective for PDGF-BB over other proteins, and exhibited potential use in biological sample analysis. Moreover, the versatility of the nanomachine was demonstrated by adapting the nanomachine for the detection of thrombin by simply changing the pre-procedure module.

AB - The novel iridium(III) complex 1 was found to be highly selective for G-quadruplex DNA, and was employed for the development of a versatile nanomachine. In the nanomachine, the single-stranded DNA (ssDNA) oligonucleotide ON1 hybridizes with the hairpin DNA oligonucleotide ON2, leading to the formation of a 5'-recessed double-stranded DNA (dsDNA) structure and the simultaneous release of a G-rich DNA sequence, which folds into a G-quadruplex motif that is recognized by complex 1. Upon the addition of Exo III, the dsDNA substrate is digested leading to the liberation of ON1 ssDNA, which enters a new cycle of the nanomachine. This nanomachine was successfully used to detect PDGF-BB by combination with a simple pre-procedure module, and exhibited a linear response between luminescence intensity and PDGF-BB concentration in the range of 10 to 300 pM (R2 = 0.992), with a limit of detection for PDGF-BB of 10 pM. This assay was highly selective for PDGF-BB over other proteins, and exhibited potential use in biological sample analysis. Moreover, the versatility of the nanomachine was demonstrated by adapting the nanomachine for the detection of thrombin by simply changing the pre-procedure module.

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SN - 0956-5663

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ER -

A versatile nanomachine for the sensitive detection of platelet-derived growth factor-BB utilizing a G-quadruplex-selective iridium(III) complex

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