@article{3e8b46e715db4671b02b89d4e973064b,
title = "A tutorial review for employing enzymes for the construction of G-quadruplex-based sensing platforms",
abstract = "With rapid advances in the field of DNA chemistry, nucleic acids and DNA-modifying enzymes have recently emerged as versatile components for the construction of oligonucleotide-based sensors. Meanwhile, the G-quadruplex motif has been widely employed for the development of DNA-based assays due to its diverse structural variety. In this tutorial, we introduce the principles of G-quadruplex-based sensing and the use of DNA-modifying enzymes for sensor platform development. We also highlight recent studies of the application of DNA-modifying enzymes for the development of G-quadruplex-based luminescent detection platforms with a view towards how those enzymes play an important role in sensitivity enhancement.",
keywords = "Enzyme, G-quadruplex, Oligonucleotide-based sensor, Sensor construction",
author = "Ma, \{Dik Lung\} and Wanhe Wang and Zhifeng Mao and Chao Yang and Chen, \{Xiu Ping\} and Lu, \{Jin Jian\} and Han, \{Quan Bin\} and Leung, \{Chung Hang\}",
note = "Funding Information: This work is supported by Hong Kong Baptist University (FRG2/14–15/004), the Health and Medical Research Fund (HMRF/13121482 and HMRF/14130522), the Research Grants Council (HKBU/12301115, HKBU/204612 and HKBU/201913), the French Agence Nationale de la Recherche/Research Grants Council Joint Research Scheme (A-HKBU201/12; Oligoswitch ANR-12-IS07-0001), National Natural Science Foundation of China (21575121), Guangdong Province Natural Science Foundation (2015A030313816), Hong Kong Baptist University Century Club Sponsorship Scheme 2015, Interdisciplinary Research Matching Scheme (RC-IRMS/14–15/06), the Science and Technology Development Fund, Macao SAR (098/2014/A2) and the University of Macau (MYRG091(Y3-L2)-ICMS12-LCH, MYRG2015-00137-ICMS-QRCM, MRG023/LCH/2013/ICMS and MRG044/LCH/2015/ICMS). Publisher copyright: {\textcopyright} 2016 Elsevier B.V.",
year = "2016",
month = mar,
day = "24",
doi = "10.1016/j.aca.2016.01.043",
language = "English",
volume = "913",
pages = "41--54",
journal = "Analytica Chimica Acta",
issn = "0003-2670",
publisher = "Elsevier B.V.",
}