A tutorial review for employing enzymes for the construction of G-quadruplex-based sensing platforms

Edmond Dik Lung MA*, Wanhe Wang, Zhifeng Mao, Chao Yang, Xiu Ping Chen, Jin Jian Lu, Simon Q B HAN, Chung Hang Leung

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

17 Citations (Scopus)

Abstract

With rapid advances in the field of DNA chemistry, nucleic acids and DNA-modifying enzymes have recently emerged as versatile components for the construction of oligonucleotide-based sensors. Meanwhile, the G-quadruplex motif has been widely employed for the development of DNA-based assays due to its diverse structural variety. In this tutorial, we introduce the principles of G-quadruplex-based sensing and the use of DNA-modifying enzymes for sensor platform development. We also highlight recent studies of the application of DNA-modifying enzymes for the development of G-quadruplex-based luminescent detection platforms with a view towards how those enzymes play an important role in sensitivity enhancement.

Original languageEnglish
Pages (from-to)41-54
Number of pages14
JournalAnalytica Chimica Acta
Volume913
DOIs
Publication statusPublished - 24 Mar 2016

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

User-Defined Keywords

  • Enzyme
  • G-quadruplex
  • Oligonucleotide-based sensor
  • Sensor construction

Fingerprint

Dive into the research topics of 'A tutorial review for employing enzymes for the construction of G-quadruplex-based sensing platforms'. Together they form a unique fingerprint.

Cite this