A traditional Chinese medicine formula inhibits tumor growth in mice and regulates the miR-34b/c-Met/β-catenin pathway

Ya Ping Wang, Xiuqiong FU, Cheng Le Yin, Ji Yao Chou, Yu Xi Liu, Jing Xuan Bai, Ying Jie Chen, Ying Wu, Jia Ying Wu, Xiao Qi Wang, Bin Liu, Zhiling YU*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Ethnopharmacology relevance: Si-Jun-Zi-Tang (SJZT) is a traditional Chinese medicine formula used to treat chronic and debilitating diseases including melanoma. SJZT-based therapies have achieved good clinical outcomes in melanoma management. However, the pharmacological basis of SJZT for its clinical use in melanoma treatment is not fully understood. Aim of the study: To investigate the anti-melanoma effects and mechanism of action of an ethanolic extract of SJZT. Materials and methods: SJZT was extracted using 50% ethanol. A murine B16 melanoma-bearing mouse model was employed to investigate the anti-melanoma effects of SJZT. microRNA (miRNA) and mRNA levels were examined by RT-qPCR, and protein levels were measured by Western blotting. Results: SJZT significantly inhibited B16 tumor growth in mice. Mechanistic investigations revealed that SJZT elevated miR-34b (a tumor suppressing miRNA), and lowered c-Met (a miR-34b target gene) and β-catenin (a downstream molecule of c-Met signaling) expression levels in the B16 tumors. Conclusions: In this study we found, for the first time, that SJZT exerts anti-melanoma effects and regulates the miR-34b/c-Met/β-catenin pathway in a melanoma mouse model. Our findings provide pharmacological justifications for the clinical use of SJZT in treating melanoma.

Original languageEnglish
Article number113065
JournalJournal of Ethnopharmacology
Volume260
DOIs
Publication statusPublished - 5 Oct 2020

Scopus Subject Areas

  • Pharmacology
  • Drug Discovery

User-Defined Keywords

  • c-Met
  • Melanoma
  • miR-34b
  • Si-Jun-Zi-Tang
  • Traditional Chinese medicine
  • β-catenin

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