A traditional Chinese medicine formula inhibits tumor growth in mice and regulates the miR-34b/c-Met/β-catenin pathway

Ya Ping Wang; Xiu Qiong Fu; Cheng Le Yin; Ji Yao Chou; Yu Xi Liu; Jing Xuan Bai; Ying Jie Chen; Ying Wu; Jia Ying Wu; Xiao Qi Wang; Bin Liu; Zhi Ling Yu

doi:10.1016/j.jep.2020.113065

A traditional Chinese medicine formula inhibits tumor growth in mice and regulates the miR-34b/c-Met/β-catenin pathway

Ya Ping Wang, Xiu Qiong Fu, Cheng Le Yin, Ji Yao Chou, Yu Xi Liu, Jing Xuan Bai, Ying Jie Chen, Ying Wu, Jia Ying Wu, Xiao Qi Wang, Bin Liu^*, Zhi Ling Yu^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

7 Citations (Scopus)

Abstract

Ethnopharmacology relevance: Si-Jun-Zi-Tang (SJZT) is a traditional Chinese medicine formula used to treat chronic and debilitating diseases including melanoma. SJZT-based therapies have achieved good clinical outcomes in melanoma management. However, the pharmacological basis of SJZT for its clinical use in melanoma treatment is not fully understood.

Aim of the study: To investigate the anti-melanoma effects and mechanism of action of an ethanolic extract of SJZT.

Materials and methods: SJZT was extracted using 50% ethanol. A murine B16 melanoma-bearing mouse model was employed to investigate the anti-melanoma effects of SJZT. microRNA (miRNA) and mRNA levels were examined by RT-qPCR, and protein levels were measured by Western blotting.

Results: SJZT significantly inhibited B16 tumor growth in mice. Mechanistic investigations revealed that SJZT elevated miR-34b (a tumor suppressing miRNA), and lowered c-Met (a miR-34b target gene) and β-catenin (a downstream molecule of c-Met signaling) expression levels in the B16 tumors.

Conclusions: In this study we found, for the first time, that SJZT exerts anti-melanoma effects and regulates the miR-34b/c-Met/β-catenin pathway in a melanoma mouse model. Our findings provide pharmacological justifications for the clinical use of SJZT in treating melanoma.

Original language	English
Article number	113065
Number of pages	4
Journal	Journal of Ethnopharmacology
Volume	260
DOIs	https://doi.org/10.1016/j.jep.2020.113065
Publication status	Published - 5 Oct 2020

Scopus Subject Areas

Pharmacology
Drug Discovery

User-Defined Keywords

c-Met
Melanoma
miR-34b
Si-Jun-Zi-Tang
Traditional Chinese medicine
β-catenin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jep.2020.113065

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@article{13953681d4924ce59bd2df10ff5b019a,

title = "A traditional Chinese medicine formula inhibits tumor growth in mice and regulates the miR-34b/c-Met/β-catenin pathway",

abstract = "Ethnopharmacology relevance: Si-Jun-Zi-Tang (SJZT) is a traditional Chinese medicine formula used to treat chronic and debilitating diseases including melanoma. SJZT-based therapies have achieved good clinical outcomes in melanoma management. However, the pharmacological basis of SJZT for its clinical use in melanoma treatment is not fully understood.Aim of the study: To investigate the anti-melanoma effects and mechanism of action of an ethanolic extract of SJZT.Materials and methods: SJZT was extracted using 50% ethanol. A murine B16 melanoma-bearing mouse model was employed to investigate the anti-melanoma effects of SJZT. microRNA (miRNA) and mRNA levels were examined by RT-qPCR, and protein levels were measured by Western blotting.Results: SJZT significantly inhibited B16 tumor growth in mice. Mechanistic investigations revealed that SJZT elevated miR-34b (a tumor suppressing miRNA), and lowered c-Met (a miR-34b target gene) and β-catenin (a downstream molecule of c-Met signaling) expression levels in the B16 tumors.Conclusions: In this study we found, for the first time, that SJZT exerts anti-melanoma effects and regulates the miR-34b/c-Met/β-catenin pathway in a melanoma mouse model. Our findings provide pharmacological justifications for the clinical use of SJZT in treating melanoma.",

keywords = "c-Met, Melanoma, miR-34b, Si-Jun-Zi-Tang, Traditional Chinese medicine, β-catenin",

author = "Wang, {Ya Ping} and Fu, {Xiu Qiong} and Yin, {Cheng Le} and Chou, {Ji Yao} and Liu, {Yu Xi} and Bai, {Jing Xuan} and Chen, {Ying Jie} and Ying Wu and Wu, {Jia Ying} and Wang, {Xiao Qi} and Bin Liu and Yu, {Zhi Ling}",

note = "Funding Information: This work was supported by Natural Science Foundation of Guangdong Province (2016 A030313007); Health and Medical Research Fund (14150571 and 15163441); National Natural Science Foundation of China (81673649, 8187141799 and 81803788); Science, Technology and Innovation Commission of Shenzhen (JCYJ20160229210327924 and JCYJ20170817173608483); Research Grants Council of Hong Kong (12125116 and 12102918); and Hong Kong Baptist University (FRG2/17-18/032). Publisher copyright: {\textcopyright} 2020 Published by Elsevier B.V.",

year = "2020",

month = oct,

day = "5",

doi = "10.1016/j.jep.2020.113065",

language = "English",

volume = "260",

journal = "Journal of Ethnopharmacology",

issn = "0378-8741",

publisher = "Elsevier",

}

TY - JOUR

T1 - A traditional Chinese medicine formula inhibits tumor growth in mice and regulates the miR-34b/c-Met/β-catenin pathway

AU - Wang, Ya Ping

AU - Fu, Xiu Qiong

AU - Yin, Cheng Le

AU - Chou, Ji Yao

AU - Liu, Yu Xi

AU - Bai, Jing Xuan

AU - Chen, Ying Jie

AU - Wu, Ying

AU - Wu, Jia Ying

AU - Wang, Xiao Qi

AU - Liu, Bin

AU - Yu, Zhi Ling

N1 - Funding Information: This work was supported by Natural Science Foundation of Guangdong Province (2016 A030313007); Health and Medical Research Fund (14150571 and 15163441); National Natural Science Foundation of China (81673649, 8187141799 and 81803788); Science, Technology and Innovation Commission of Shenzhen (JCYJ20160229210327924 and JCYJ20170817173608483); Research Grants Council of Hong Kong (12125116 and 12102918); and Hong Kong Baptist University (FRG2/17-18/032). Publisher copyright: © 2020 Published by Elsevier B.V.

PY - 2020/10/5

Y1 - 2020/10/5

N2 - Ethnopharmacology relevance: Si-Jun-Zi-Tang (SJZT) is a traditional Chinese medicine formula used to treat chronic and debilitating diseases including melanoma. SJZT-based therapies have achieved good clinical outcomes in melanoma management. However, the pharmacological basis of SJZT for its clinical use in melanoma treatment is not fully understood.Aim of the study: To investigate the anti-melanoma effects and mechanism of action of an ethanolic extract of SJZT.Materials and methods: SJZT was extracted using 50% ethanol. A murine B16 melanoma-bearing mouse model was employed to investigate the anti-melanoma effects of SJZT. microRNA (miRNA) and mRNA levels were examined by RT-qPCR, and protein levels were measured by Western blotting.Results: SJZT significantly inhibited B16 tumor growth in mice. Mechanistic investigations revealed that SJZT elevated miR-34b (a tumor suppressing miRNA), and lowered c-Met (a miR-34b target gene) and β-catenin (a downstream molecule of c-Met signaling) expression levels in the B16 tumors.Conclusions: In this study we found, for the first time, that SJZT exerts anti-melanoma effects and regulates the miR-34b/c-Met/β-catenin pathway in a melanoma mouse model. Our findings provide pharmacological justifications for the clinical use of SJZT in treating melanoma.

AB - Ethnopharmacology relevance: Si-Jun-Zi-Tang (SJZT) is a traditional Chinese medicine formula used to treat chronic and debilitating diseases including melanoma. SJZT-based therapies have achieved good clinical outcomes in melanoma management. However, the pharmacological basis of SJZT for its clinical use in melanoma treatment is not fully understood.Aim of the study: To investigate the anti-melanoma effects and mechanism of action of an ethanolic extract of SJZT.Materials and methods: SJZT was extracted using 50% ethanol. A murine B16 melanoma-bearing mouse model was employed to investigate the anti-melanoma effects of SJZT. microRNA (miRNA) and mRNA levels were examined by RT-qPCR, and protein levels were measured by Western blotting.Results: SJZT significantly inhibited B16 tumor growth in mice. Mechanistic investigations revealed that SJZT elevated miR-34b (a tumor suppressing miRNA), and lowered c-Met (a miR-34b target gene) and β-catenin (a downstream molecule of c-Met signaling) expression levels in the B16 tumors.Conclusions: In this study we found, for the first time, that SJZT exerts anti-melanoma effects and regulates the miR-34b/c-Met/β-catenin pathway in a melanoma mouse model. Our findings provide pharmacological justifications for the clinical use of SJZT in treating melanoma.

KW - c-Met

KW - Melanoma

KW - miR-34b

KW - Si-Jun-Zi-Tang

KW - Traditional Chinese medicine

KW - β-catenin

UR - http://www.scopus.com/inward/record.url?scp=85086391613&partnerID=8YFLogxK

U2 - 10.1016/j.jep.2020.113065

DO - 10.1016/j.jep.2020.113065

M3 - Journal article

C2 - 32505839

AN - SCOPUS:85086391613

SN - 0378-8741

VL - 260

JO - Journal of Ethnopharmacology

JF - Journal of Ethnopharmacology

M1 - 113065

ER -

A traditional Chinese medicine formula inhibits tumor growth in mice and regulates the miR-34b/c-Met/β-catenin pathway

Abstract

Scopus Subject Areas

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UN SDGs

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