A thermostable trypsin inhibitor with antiproliferative activity from small pinto beans

Yau Sang Chan, Yan Bo Zhang*, Stephen Cho Wing Sze, Tzi Bun Ng*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

12 Citations (Scopus)


Small pinto bean is a cultivar of Phaseolus vulgaris. It produces a 16-kDa trypsin inhibitor that could be purified using anion exchange and size chromatography. Q-Sepharose, Mono Q and Superdex 75 columns were employed for the isolation process. Small pinto bean trypsin inhibitor demonstrated moderate pH stability (pH 2–10) and marked heat stability, with its trypsin inhibitory activity largely retained after exposure to 100 °C for half an hour. The activity was abolished in the presence of dithiothreitol, in a dose-dependent manner, implying that disulfide bonds in small pinto bean trypsin inhibitor are crucial for the activity. The trypsin inhibitor showed a blocked N-terminus. The trypsin inhibitor only slightly inhibited the viability of breast cancer MCF7 and hepatoma HepG2 cells at 125 μM.
Original languageEnglish
Pages (from-to)485-490
Number of pages6
JournalJournal of Enzyme Inhibition and Medicinal Chemistry
Issue number4
Early online date16 Jul 2013
Publication statusPublished - Aug 2014

Scopus Subject Areas

  • Medicine(all)

User-Defined Keywords

  • Bowman--Birk
  • dithiothreitol
  • leguminous
  • Phaseolus vulgaris


Dive into the research topics of 'A thermostable trypsin inhibitor with antiproliferative activity from small pinto beans'. Together they form a unique fingerprint.

Cite this