Abstract
Background: Rheumatoid arthritis (RA) is a chronic autoimmune disease. In China. Leigongteng Duogan Tablet (LDT) is the mainstay in RA treatment owing to its excellent efficacy and low price. However, long-term application of LDT causes hepatotoxicity in patients. Oxidative stress is the main toxicological mechanism of this drug. An herbal formula comprising Rosae Multiflorae Fmctus and Lonicerae Japonicae Flos (RL). is a traditional remedy for treating inflammation-related diseases in Cliina. We have previously found that an ethanolic extract of RL (RLE) has anti-RA effects in rats. RLE contains multiple antioxidant compounds such as chlorogenic acid, gallic acid, and kaempferol, therefore possibly being able to counteract LDT-induced toxicity.
Objectives: This study aimed to investigate the anti-RA effects of the combination of RLE and LDT (RLE-plus-LDT). and to determine whether RLE can reduce LDT-induced hepatotoxicity.
Methods: A CIA (Collagen-induced arthritis) mouse model was used to evaluate the anti-RA effects of RLE-plus-LDT. X-ray analysis was used to obseive bone erosion in mice. ELISA assays were employed to examine protein levels. Flow cytometry was used to examine the proportion of Thl7 cells in mouse splenocytes.
Results: Intragastric administration of RLE-plus-LDT attenuated joint swelling of CIA mice. X-ray results showed that RLE-plus-LDT suppressed bone erosion. RLE-plus-LDT down-regulated serum levels of the pro-inflammatory cytokines IL-6 and IL-ip and reduced splenic Th 17 cell proportion in CIA mice. Also. RLE lowered LDT-induced hepatotoxicity evidenced by lowered serum aspartate aminotransferase levels.
Conclusions: To summarize, RLE-plus-LDT significantly ameliorated CIA in mice. RLE reduced LDT-induced hepatotoxicity. This study provides a pharmacological basis for developing RLE into an adjuvant ding of LDT for treating RA. This study w as supported by the grant HMRF19200791.
Objectives: This study aimed to investigate the anti-RA effects of the combination of RLE and LDT (RLE-plus-LDT). and to determine whether RLE can reduce LDT-induced hepatotoxicity.
Methods: A CIA (Collagen-induced arthritis) mouse model was used to evaluate the anti-RA effects of RLE-plus-LDT. X-ray analysis was used to obseive bone erosion in mice. ELISA assays were employed to examine protein levels. Flow cytometry was used to examine the proportion of Thl7 cells in mouse splenocytes.
Results: Intragastric administration of RLE-plus-LDT attenuated joint swelling of CIA mice. X-ray results showed that RLE-plus-LDT suppressed bone erosion. RLE-plus-LDT down-regulated serum levels of the pro-inflammatory cytokines IL-6 and IL-ip and reduced splenic Th 17 cell proportion in CIA mice. Also. RLE lowered LDT-induced hepatotoxicity evidenced by lowered serum aspartate aminotransferase levels.
Conclusions: To summarize, RLE-plus-LDT significantly ameliorated CIA in mice. RLE reduced LDT-induced hepatotoxicity. This study provides a pharmacological basis for developing RLE into an adjuvant ding of LDT for treating RA. This study w as supported by the grant HMRF19200791.
| Original language | English |
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| Publication status | Published - 23 Mar 2024 |
| Event | Annual Scientific Meeting of Hong Kong Society for Immunology (HKSI) 2024 - Duration: 23 Mar 2024 → … |
Conference
| Conference | Annual Scientific Meeting of Hong Kong Society for Immunology (HKSI) 2024 |
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| Period | 23/03/24 → … |
