A systematic approach for authentication of medicinal Patrinia species using an integration of morphological, chemical and molecular methods

Kwan-Ho Wong, Tao Zhang, Grace Gar Lee Yue, Man-Ching Li, Hoi Yan Wu, Man Ho Tong, Xin-Lei Zhao, Hubiao Chen, Clara Bik San Lau*, Pang Chui Shaw*, David Tai Wai Lau*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

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Abstract

Four common Patrinia species, including P. heterophylla, P. monandra, P. scabiosifolia and P. villosa, have been documented as herbal medicines with various clinical applications, such as anti-cancer, anti-diarrhea and sedative. However, the authentication of medicinal Patrinia species poses a problem, particularly with the processed herbal materials. This study aimed to systematically authenticate the four medicinal Patrinia species in the market using morphological and chemical characterization, as well as DNA markers. We found the species identity authenticated by traditional morphologies were in good agreement with both chemical and molecular results. The four species showed species-specific patterns in chromatographic profiles with distinct chemical markers. We also revealed the power of complete chloroplast genomes in species authentication. The sequences of targeted loci, namely atpB, petA, rpl2-rpl23 and psaI-ycf4, contained informative nucleotides for the species differentiation. Our results also facilitate authentication of medicinal Patrinia species using new DNA barcoding markers. To the best of our knowledge, this is the first report on the application of morphology, chemical fingerprinting, complete chloroplast genomes and species-specific Insertion-Deletions (InDels) in differentiating Patrinia species. This study reported on the power of a systematic, multidisciplinary approach in authenticating medicinal Patrinia species.
Original languageEnglish
Article number6566
Number of pages14
JournalScientific Reports
Volume14
Issue number1
DOIs
Publication statusPublished - 19 Mar 2024

Scopus Subject Areas

  • Biochemistry, Genetics and Molecular Biology(all)

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