TY - JOUR
T1 - A systematic approach for authentication of medicinal Patrinia species using an integration of morphological, chemical and molecular methods
AU - Wong, Kwan-Ho
AU - Zhang, Tao
AU - Yue, Grace Gar Lee
AU - Li, Man-Ching
AU - Wu, Hoi Yan
AU - Tong, Man Ho
AU - Zhao, Xin-Lei
AU - Chen, Hubiao
AU - Lau, Clara Bik San
AU - Shaw, Pang Chui
AU - Lau, David Tai Wai
N1 - Publisher Copyright:
© The Author(s) 2024.
PY - 2024/3/19
Y1 - 2024/3/19
N2 - Four common Patrinia species, including P. heterophylla, P. monandra, P. scabiosifolia and P. villosa, have been documented as herbal medicines with various clinical applications, such as anti-cancer, anti-diarrhea and sedative. However, the authentication of medicinal Patrinia species poses a problem, particularly with the processed herbal materials. This study aimed to systematically authenticate the four medicinal Patrinia species in the market using morphological and chemical characterization, as well as DNA markers. We found the species identity authenticated by traditional morphologies were in good agreement with both chemical and molecular results. The four species showed species-specific patterns in chromatographic profiles with distinct chemical markers. We also revealed the power of complete chloroplast genomes in species authentication. The sequences of targeted loci, namely atpB, petA, rpl2-rpl23 and psaI-ycf4, contained informative nucleotides for the species differentiation. Our results also facilitate authentication of medicinal Patrinia species using new DNA barcoding markers. To the best of our knowledge, this is the first report on the application of morphology, chemical fingerprinting, complete chloroplast genomes and species-specific Insertion-Deletions (InDels) in differentiating Patrinia species. This study reported on the power of a systematic, multidisciplinary approach in authenticating medicinal Patrinia species.
AB - Four common Patrinia species, including P. heterophylla, P. monandra, P. scabiosifolia and P. villosa, have been documented as herbal medicines with various clinical applications, such as anti-cancer, anti-diarrhea and sedative. However, the authentication of medicinal Patrinia species poses a problem, particularly with the processed herbal materials. This study aimed to systematically authenticate the four medicinal Patrinia species in the market using morphological and chemical characterization, as well as DNA markers. We found the species identity authenticated by traditional morphologies were in good agreement with both chemical and molecular results. The four species showed species-specific patterns in chromatographic profiles with distinct chemical markers. We also revealed the power of complete chloroplast genomes in species authentication. The sequences of targeted loci, namely atpB, petA, rpl2-rpl23 and psaI-ycf4, contained informative nucleotides for the species differentiation. Our results also facilitate authentication of medicinal Patrinia species using new DNA barcoding markers. To the best of our knowledge, this is the first report on the application of morphology, chemical fingerprinting, complete chloroplast genomes and species-specific Insertion-Deletions (InDels) in differentiating Patrinia species. This study reported on the power of a systematic, multidisciplinary approach in authenticating medicinal Patrinia species.
UR - https://www.nature.com/articles/s41598-024-57115-w?_gl=1*ttl7i0*_up*MQ..&gclid=Cj0KCQjw4MSzBhC8ARIsAPFOuyWF4h9x1vzNBxAmDWVghrRnFpN6dzGyYIa62pE1JHJu1Q9RX3BxEuQaAqdOEALw_wcB
UR - http://www.scopus.com/inward/record.url?scp=85188074779&partnerID=8YFLogxK
U2 - 10.1038/s41598-024-57115-w
DO - 10.1038/s41598-024-57115-w
M3 - Journal article
SN - 2045-2322
VL - 14
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 6566
ER -