A specific and bioactive polysaccharide marker for Cordyceps

Lifeng Li; Grace Wing Chiu But; Quanwei Zhang; Man Liu; Miao Miao Chen; Xin Wen; Hoi Yan Wu; Huiyuan Cheng; Pema Tenzin Puno; Ji Xia Zhang; Hau Yee Fung; Su Ping Bai; Tin Long Wong; Zhongzhen Zhao; Hui Cao; Karl Wah Keung Tsim; Pang Chui Shaw; Quan Bin Han; Han Dong Sun

doi:10.1016/j.carbpol.2021.118343

A specific and bioactive polysaccharide marker for Cordyceps

Lifeng Li, Grace Wing Chiu But, Quanwei Zhang, Man Liu, Miao Miao Chen, Xin Wen, Hoi Yan Wu, Huiyuan Cheng, Pema Tenzin Puno, Ji Xia Zhang, Hau Yee Fung, Su Ping Bai, Tin Long Wong, Zhongzhen Zhao, Hui Cao, Karl Wah Keung Tsim, Pang Chui Shaw^*, Quan Bin Han^*, Han Dong Sun

^*Corresponding author for this work

School of Chinese Medicine

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Cordyceps is one of the most expensive and widely used functional foods. But the authenticity is still a concern due to the lack of appropriate markers. By targeting polysaccharides, this study aimed to develop a specific, and bioactive marker for Cordyceps. Firstly, the results of screening tests of 250 samples by examining both genetic markers and polysaccharide profile showed that a unique polysaccharide fraction (named CCP) was particular to the caterpillar parts. Its potential as a marker was further demonstrated by its ability to induce NO and cytokine production in RAW 264.7 cells. CCP was characterized to be an α-1,4-glucan with a branch at C-6 by the conventional structure analyzing and de novo oligosaccharides sequencing. The content of CCP was closely correlated to the traditional classification criteria. Generally, CCP was a marker that simultaneously enables qualitative and quantitative analysis of Cordyceps.

Original language	English
Article number	118343
Journal	Carbohydrate Polymers
Volume	269
DOIs	https://doi.org/10.1016/j.carbpol.2021.118343
Publication status	Published - 1 Oct 2021

Scopus Subject Areas

Organic Chemistry
Polymers and Plastics
Materials Chemistry

User-Defined Keywords

Authentication
Cordyceps
Immune activity
Marker
Polysaccharide

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10.1016/j.carbpol.2021.118343

Cite this

Li, L., But, G. W. C., Zhang, Q., Liu, M., Chen, M. M., Wen, X., Wu, H. Y., Cheng, H., Puno, P. T., Zhang, J. X., Fung, H. Y., Bai, S. P., Wong, T. L., Zhao, Z., Cao, H., Tsim, K. W. K., Shaw, P. C., Han, Q. B., & Sun, H. D. (2021). A specific and bioactive polysaccharide marker for Cordyceps. Carbohydrate Polymers, 269, [118343]. https://doi.org/10.1016/j.carbpol.2021.118343

Li, Lifeng ; But, Grace Wing Chiu ; Zhang, Quanwei ; Liu, Man ; Chen, Miao Miao ; Wen, Xin ; Wu, Hoi Yan ; Cheng, Huiyuan ; Puno, Pema Tenzin ; Zhang, Ji Xia ; Fung, Hau Yee ; Bai, Su Ping ; Wong, Tin Long ; Zhao, Zhongzhen ; Cao, Hui ; Tsim, Karl Wah Keung ; Shaw, Pang Chui ; Han, Quan Bin ; Sun, Han Dong. / A specific and bioactive polysaccharide marker for Cordyceps. In: Carbohydrate Polymers. 2021 ; Vol. 269.

@article{a16e0c4e57e54d8d800bacfcce8c8710,

title = "A specific and bioactive polysaccharide marker for Cordyceps",

abstract = "Cordyceps is one of the most expensive and widely used functional foods. But the authenticity is still a concern due to the lack of appropriate markers. By targeting polysaccharides, this study aimed to develop a specific, and bioactive marker for Cordyceps. Firstly, the results of screening tests of 250 samples by examining both genetic markers and polysaccharide profile showed that a unique polysaccharide fraction (named CCP) was particular to the caterpillar parts. Its potential as a marker was further demonstrated by its ability to induce NO and cytokine production in RAW 264.7 cells. CCP was characterized to be an α-1,4-glucan with a branch at C-6 by the conventional structure analyzing and de novo oligosaccharides sequencing. The content of CCP was closely correlated to the traditional classification criteria. Generally, CCP was a marker that simultaneously enables qualitative and quantitative analysis of Cordyceps.",

keywords = "Authentication, Cordyceps, Immune activity, Marker, Polysaccharide",

author = "Lifeng Li and But, {Grace Wing Chiu} and Quanwei Zhang and Man Liu and Chen, {Miao Miao} and Xin Wen and Wu, {Hoi Yan} and Huiyuan Cheng and Puno, {Pema Tenzin} and Zhang, {Ji Xia} and Fung, {Hau Yee} and Bai, {Su Ping} and Wong, {Tin Long} and Zhongzhen Zhao and Hui Cao and Tsim, {Karl Wah Keung} and Shaw, {Pang Chui} and Han, {Quan Bin} and Sun, {Han Dong}",

note = "Funding Information: This work was funded and supported by HKSAR Innovation and Technology Fund (ITF), Tier 3, ITS/311/09 , General Research Fund ( 12100615 , 22100014 , 12100818 ), UGC Research Matching Grant Scheme ( 2019-1-10 , 2019-1-14 , 2019-2-06 ), Health Medical Research Fund (11122531, 14150521, 17182681), National Natural Science Foundation of China ( 81473341 ), the Science and Technology Project of Shenzhen ( JCYJ20160531193812867 ), the Key-Area Research and Development Program of Guangdong Province ( 2020B1111110007 ), Hong Kong Baptist University (RC-Start-up Grants, MPCF-001-2016/2017 , RC-IRMS/14-15/06 , FRG2/17-18/060 and FRG2/16-17/002 ), and Vincent & Lily Woo Foundation . Publisher Copyright: {\textcopyright} 2021 Elsevier Ltd",

year = "2021",

month = oct,

day = "1",

doi = "10.1016/j.carbpol.2021.118343",

language = "English",

volume = "269",

journal = "Carbohydrate Polymers",

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publisher = "Elsevier Ltd.",

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A specific and bioactive polysaccharide marker for Cordyceps. / Li, Lifeng; But, Grace Wing Chiu; Zhang, Quanwei; Liu, Man; Chen, Miao Miao; Wen, Xin; Wu, Hoi Yan; Cheng, Huiyuan; Puno, Pema Tenzin; Zhang, Ji Xia; Fung, Hau Yee; Bai, Su Ping; Wong, Tin Long; Zhao, Zhongzhen; Cao, Hui; Tsim, Karl Wah Keung; Shaw, Pang Chui; Han, Quan Bin; Sun, Han Dong.

In: Carbohydrate Polymers, Vol. 269, 118343, 01.10.2021.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - A specific and bioactive polysaccharide marker for Cordyceps

AU - Li, Lifeng

AU - But, Grace Wing Chiu

AU - Zhang, Quanwei

AU - Liu, Man

AU - Chen, Miao Miao

AU - Wen, Xin

AU - Wu, Hoi Yan

AU - Cheng, Huiyuan

AU - Puno, Pema Tenzin

AU - Zhang, Ji Xia

AU - Fung, Hau Yee

AU - Bai, Su Ping

AU - Wong, Tin Long

AU - Zhao, Zhongzhen

AU - Cao, Hui

AU - Tsim, Karl Wah Keung

AU - Shaw, Pang Chui

AU - Han, Quan Bin

AU - Sun, Han Dong

N1 - Funding Information: This work was funded and supported by HKSAR Innovation and Technology Fund (ITF), Tier 3, ITS/311/09 , General Research Fund ( 12100615 , 22100014 , 12100818 ), UGC Research Matching Grant Scheme ( 2019-1-10 , 2019-1-14 , 2019-2-06 ), Health Medical Research Fund (11122531, 14150521, 17182681), National Natural Science Foundation of China ( 81473341 ), the Science and Technology Project of Shenzhen ( JCYJ20160531193812867 ), the Key-Area Research and Development Program of Guangdong Province ( 2020B1111110007 ), Hong Kong Baptist University (RC-Start-up Grants, MPCF-001-2016/2017 , RC-IRMS/14-15/06 , FRG2/17-18/060 and FRG2/16-17/002 ), and Vincent & Lily Woo Foundation . Publisher Copyright: © 2021 Elsevier Ltd

PY - 2021/10/1

Y1 - 2021/10/1

N2 - Cordyceps is one of the most expensive and widely used functional foods. But the authenticity is still a concern due to the lack of appropriate markers. By targeting polysaccharides, this study aimed to develop a specific, and bioactive marker for Cordyceps. Firstly, the results of screening tests of 250 samples by examining both genetic markers and polysaccharide profile showed that a unique polysaccharide fraction (named CCP) was particular to the caterpillar parts. Its potential as a marker was further demonstrated by its ability to induce NO and cytokine production in RAW 264.7 cells. CCP was characterized to be an α-1,4-glucan with a branch at C-6 by the conventional structure analyzing and de novo oligosaccharides sequencing. The content of CCP was closely correlated to the traditional classification criteria. Generally, CCP was a marker that simultaneously enables qualitative and quantitative analysis of Cordyceps.

AB - Cordyceps is one of the most expensive and widely used functional foods. But the authenticity is still a concern due to the lack of appropriate markers. By targeting polysaccharides, this study aimed to develop a specific, and bioactive marker for Cordyceps. Firstly, the results of screening tests of 250 samples by examining both genetic markers and polysaccharide profile showed that a unique polysaccharide fraction (named CCP) was particular to the caterpillar parts. Its potential as a marker was further demonstrated by its ability to induce NO and cytokine production in RAW 264.7 cells. CCP was characterized to be an α-1,4-glucan with a branch at C-6 by the conventional structure analyzing and de novo oligosaccharides sequencing. The content of CCP was closely correlated to the traditional classification criteria. Generally, CCP was a marker that simultaneously enables qualitative and quantitative analysis of Cordyceps.

KW - Authentication

KW - Cordyceps

KW - Immune activity

KW - Marker

KW - Polysaccharide

UR - http://www.scopus.com/inward/record.url?scp=85108450157&partnerID=8YFLogxK

U2 - 10.1016/j.carbpol.2021.118343

DO - 10.1016/j.carbpol.2021.118343

M3 - Article

C2 - 34294350

AN - SCOPUS:85108450157

VL - 269

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

SN - 0144-8617

M1 - 118343

ER -

A specific and bioactive polysaccharide marker for Cordyceps

Abstract

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