TY - JOUR
T1 - A single marker choice strategy in simultaneous characterization and quantification of multiple components by rapid resolution liquid chromatography coupled with triple quadrupole tandem mass spectrometry (RRLC-QqQ-MS)
AU - Ning, Zhangchi
AU - Liu, Zhenli
AU - Song, Zhiqian
AU - Zhao, Siyu
AU - Dong, Yunzhuo
AU - Zeng, Honglian
AU - Shu, Yisong
AU - Lu, Cheng
AU - Liu, Yuanyan
AU - LYU, Aiping
N1 - Funding Information:
This work was supported by the National Natural Science Foundation committee of China (Project No. 81573569, 81470177), Beijing Nova Program (Project No. xx2014B073), 973 Program (Project No. 2012CB518401).
PY - 2016/5/30
Y1 - 2016/5/30
N2 - Single standard to determine multi-components (SSDMC) method has been accepted as an efficient technique for the quality control of Traditional Chinese medicines (TCMs), especially for overcoming the shortage of reference standards. HPLC-UV methods have been applied to establish SSDMC method for quantitative analysis in several plant medicines and Chinese patent medicines, however, no LC-MS methods have been used. The purpose of this study is to put forward an improved strategy for the choice of single marker in SSDMC using rapid resolution liquid chromatography coupled with triple quadrupole tandem mass spectrometry (RRLC-QqQ-MS). Five different Panax genus plants, recorded in the Chinese Pharmacopeia 2015 edition, were used as research subjects. An improved SSDMC strategy for simultaneous characterization and determination of 18 bioactive saponins in five Panax plants was put forward, and which was validated to be more superior. Then, it was fully investigated with respect to linearity, LODs, LOQs, precision and accuracy. Coupling with multivariate statistical analysis, the established and validated SSDMC strategy could be successively used in discrimination of the five Panax genus plants.
AB - Single standard to determine multi-components (SSDMC) method has been accepted as an efficient technique for the quality control of Traditional Chinese medicines (TCMs), especially for overcoming the shortage of reference standards. HPLC-UV methods have been applied to establish SSDMC method for quantitative analysis in several plant medicines and Chinese patent medicines, however, no LC-MS methods have been used. The purpose of this study is to put forward an improved strategy for the choice of single marker in SSDMC using rapid resolution liquid chromatography coupled with triple quadrupole tandem mass spectrometry (RRLC-QqQ-MS). Five different Panax genus plants, recorded in the Chinese Pharmacopeia 2015 edition, were used as research subjects. An improved SSDMC strategy for simultaneous characterization and determination of 18 bioactive saponins in five Panax plants was put forward, and which was validated to be more superior. Then, it was fully investigated with respect to linearity, LODs, LOQs, precision and accuracy. Coupling with multivariate statistical analysis, the established and validated SSDMC strategy could be successively used in discrimination of the five Panax genus plants.
KW - Panax plants
KW - Partial least squares discriminant analysis (PLS-DA)
KW - Principal component analysis (PCA)
KW - Quantitative analysis of multi-components with single marker (SSDMC)
KW - Rapid resolution liquid chromatography coupled with triple quadrupoletandem mass spectrometry (RRLC-QqQ-MS)
UR - http://www.scopus.com/inward/record.url?scp=84959268262&partnerID=8YFLogxK
U2 - 10.1016/j.jpba.2016.02.039
DO - 10.1016/j.jpba.2016.02.039
M3 - Journal article
C2 - 26955755
AN - SCOPUS:84959268262
SN - 0731-7085
VL - 124
SP - 174
EP - 188
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
ER -