A simple method to optimize the HSCCC two-phase solvent system by predicting the partition coefficient for target compound

Quan Bin Han, Lina Wong, Nian Yun Yang, Jing Zheng Song, Chun Feng Qiao, Hillary Yiu, Yoichiro Ito, Hong Xi Xu*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

55 Citations (Scopus)

Abstract

A simple method was developed to optimize the solvent ratio of the two-phase solvent system used in the high-speed counter-current chromatography (HSCCC) separation. Some mathematic equations, such as the exponential and the power equations, were established to describe the relationship between the solvent ratio and the partition coefficient. Using this new method, the two-phase solvent system was easily optimized to obtain a proper partition coefficient for the CCC separation of the target compound. Furthermore, this method was satisfactorily applied in determining the two-phase solvent system for the HSCCC preparation of pseudolaric acid B from the Chinese herb Pseudolarix kaempferi Gordon (Pinaceae). The two-phase solvent system of n-hexane/EtOAc/MeOH/H20 (5:5:5:5 by volume) was used with a good partition coefficient K = 1.08. As a result, 232.05 mg of pseudolaric acid B was yielded from 0.5 g of the crude extract with a purity of 97.26% by HPLC analysis.

Original languageEnglish
Pages (from-to)1189-1194
Number of pages6
JournalJournal of Separation Science
Volume31
Issue number6-7
DOIs
Publication statusPublished - Apr 2008

Scopus Subject Areas

  • Analytical Chemistry
  • Filtration and Separation

User-Defined Keywords

  • High-speed counter-current chromatography
  • Partition coefficient
  • Preparative isolation
  • Pseudolaric acid B
  • Pseudolarix kaempferi Gordon (Pinaceae)

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