A simple fluorometric method using chlorophyll a for determination of Hg2+ ion

Shangfen Gao, Guangqun Tan, Hongyan Yuan, Dan Xiao*, Martin M F CHOI

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

19 Citations (Scopus)


A simple and green analytical procedure based on chlorophyll a is presented for the determination of Hg2+ ion. Chlorophyll a was extracted and purified from the leaves of pea and is employed as a reagent for analysis of Hg2+ ion. It displays remarkable fluorescence emission at 674 nm when excited at 412 nm. The emission intensity decreased significantly on exposure to various concentrations of Hg2+ ion. This forms the basis for the determination of Hg2+ ion. The proposed method was evaluated for sensitivity and selectivity. The linear concentration range was found to be 2.0-10 μM with r 2 = 0.997 and the limit of detection for Hg 2+ ion was 1.3 μM. Ions including Pb2+, Cd 2+, Ag+, Zn2+, Co2+, Ni 2+, Cu2+, Mg2+, Mn2+, Ru 3+, Er3+, K+, Na+, NH4 +, Cl-, NO3 -, CH 3COO- and SO4 2- did not interfere with the measurement of Hg2+ ion even at 500-fold excess. Since chlorophyll a is widely available in the leaves of most plants, and the extraction and purification process is simple, this technique can provide an alternative, sensitive and economical way to determine Hg2+ ion.

Original languageEnglish
Pages (from-to)159-162
Number of pages4
JournalMicrochimica Acta
Issue number3-4
Publication statusPublished - Feb 2006

Scopus Subject Areas

  • Analytical Chemistry

User-Defined Keywords

  • Chlorophyll a
  • Fluorescence
  • Hg ion


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