TY - JOUR
T1 - A sensitivity enhanced high-performance liquid chromatography fluorescence method for the detection of nephrotoxic and carcinogenic aristolochic acid in herbal medicines
AU - Chan, Wan
AU - Lee, Kim Chung
AU - Liu, Ning
AU - CAI, Zongwei
N1 - Funding Information:
We thank Dr. Martin M.F. Choi for his help in doing the static fluorescence analysis. The financial support from the Research Grant Council, University Grants Committee of Hong Kong (HKBU2459/06M) is acknowledged.
PY - 2007/9/14
Y1 - 2007/9/14
N2 - A new, sensitive and selective HPLC method with fluorescence detector (HPLC-FLD) for the determination of nephrotoxic and carcinogenic aristolochic acid (AA) in herbal medicines by using pre-column derivatization with zinc powder in acetic acid is presented. Variables governing the derivatization reaction, such as the amount of zinc powder and acetic acid, as well as the derivatization time were studied and optimized. An extended linear dynamic range over three orders of magnitude was observed for AA-I and AA-II (R2 > 0.9998). Method accuracy at low, medium and high spiked AA levels determined by the percentage mean deviation was below 4.4% and 7.2% for AA-I and AA-II, respectively. The detection limits of 0.39 ng/mL (AA-I) and 0.52 ng/mL (AA-II) were 2 orders of magnitude lower than those obtained from HPLC-MS or CE-ECD analyses, 3-4 orders of magnitude lower than those from HPLC-UV or CE-UV methods. The developed method has been applied for the determination of AA in herbal medicines. Among the tested samples, Guanmutong had the highest AA concentration (2607.0 μg/g AA-I, 711.2 μg/g AA-II). Comparison studies between HPLC-FLD and HPLC-MS/MS demonstrated that the two methods gave similar quantitative results for the selected herb samples.
AB - A new, sensitive and selective HPLC method with fluorescence detector (HPLC-FLD) for the determination of nephrotoxic and carcinogenic aristolochic acid (AA) in herbal medicines by using pre-column derivatization with zinc powder in acetic acid is presented. Variables governing the derivatization reaction, such as the amount of zinc powder and acetic acid, as well as the derivatization time were studied and optimized. An extended linear dynamic range over three orders of magnitude was observed for AA-I and AA-II (R2 > 0.9998). Method accuracy at low, medium and high spiked AA levels determined by the percentage mean deviation was below 4.4% and 7.2% for AA-I and AA-II, respectively. The detection limits of 0.39 ng/mL (AA-I) and 0.52 ng/mL (AA-II) were 2 orders of magnitude lower than those obtained from HPLC-MS or CE-ECD analyses, 3-4 orders of magnitude lower than those from HPLC-UV or CE-UV methods. The developed method has been applied for the determination of AA in herbal medicines. Among the tested samples, Guanmutong had the highest AA concentration (2607.0 μg/g AA-I, 711.2 μg/g AA-II). Comparison studies between HPLC-FLD and HPLC-MS/MS demonstrated that the two methods gave similar quantitative results for the selected herb samples.
KW - Aristolochic acid
KW - Fluorescence detection
KW - High-performance liquid chromatography
KW - Pre-column derivatization
KW - Traditional Chinese medicine
UR - http://www.scopus.com/inward/record.url?scp=34548036750&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2007.06.055
DO - 10.1016/j.chroma.2007.06.055
M3 - Journal article
C2 - 17631302
AN - SCOPUS:34548036750
SN - 0021-9673
VL - 1164
SP - 113
EP - 119
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1-2
ER -