TY - JOUR
T1 - A semi-dominant mutation in a CC-NB-LRR-type protein leads to a short-root phenotype in rice
AU - Yu, Zhiming
AU - Dong, Lixiang
AU - Jiang, Zhifang
AU - Yi, Keke
AU - ZHANG, Jianhua
AU - Zhang, Zhongchen
AU - Zhu, Zhenxing
AU - Wu, Yuhuan
AU - Xu, Maojun
AU - Ni, Jun
N1 - Funding Information:
This work was funded by the Zhejiang Provincial Natural Science Foundation of China (Grant No. LY18C060008) and the Independent Projects for Agricultural Research in Hangzhou (Grant No. 20170432B07).
Funding Information:
We thank Prof. Chuanzao Mao from Zhejiang University for technical assistance. This work was funded by the Zhejiang Provincial Natural Science Foundation of China (Grant No. LY18C060008) and the Independent Projects for Agricultural Research in Hangzhou (Grant No. 20170432B07). The data sets supporting the results of this article are included within the article.
PY - 2018/12/1
Y1 - 2018/12/1
N2 - The mechanisms of plant defense against pathogen attack in plant leaves have been extensively studied. However, our understanding of plant defense mechanisms in plant roots is still limited. In this study, a semi-dominant mutant nrtp1-D (necrotic root tip 1), with a short-root phenotype, was characterized in rice. Map-based cloning revealed that NRTP1 encoded a typical coiled-coil nucleotide binding leucine rich repeat (CC-NB-LRR) type protein and the mutation caused an amino acid substitution in the Nucleotide-Binding adaptor shared by Apaf1, certain R genes and CED4 (NB-ARC) domain, which may cause constitutive auto-activation of the NRTP1 protein. Gene expression analysis revealed that NRTP1 was preferentially expressed in rice roots. Expression of mutant nrtp1-D in tobacco leaves induced necrotic lesions, which indicated a common mechanism of plant defense response between leaves and roots. Transcriptome analysis revealed that many typical defense-response genes were differentially expressed in homozygous nrtp1-D. In addition, we also found differential expression of genes in pathways which had not previously been described as being associated with pathogen response. Histochemical analysis showed that the level of nitric oxide (NO), but not reactive oxygen species (ROS), was increased in homozygous nrtp1-D mutant roots. These results indicate that, in addition to the mechanism of defense response common to both roots and shoots, a novel pathway may also exist in rice roots, which does not operate in shoots.
AB - The mechanisms of plant defense against pathogen attack in plant leaves have been extensively studied. However, our understanding of plant defense mechanisms in plant roots is still limited. In this study, a semi-dominant mutant nrtp1-D (necrotic root tip 1), with a short-root phenotype, was characterized in rice. Map-based cloning revealed that NRTP1 encoded a typical coiled-coil nucleotide binding leucine rich repeat (CC-NB-LRR) type protein and the mutation caused an amino acid substitution in the Nucleotide-Binding adaptor shared by Apaf1, certain R genes and CED4 (NB-ARC) domain, which may cause constitutive auto-activation of the NRTP1 protein. Gene expression analysis revealed that NRTP1 was preferentially expressed in rice roots. Expression of mutant nrtp1-D in tobacco leaves induced necrotic lesions, which indicated a common mechanism of plant defense response between leaves and roots. Transcriptome analysis revealed that many typical defense-response genes were differentially expressed in homozygous nrtp1-D. In addition, we also found differential expression of genes in pathways which had not previously been described as being associated with pathogen response. Histochemical analysis showed that the level of nitric oxide (NO), but not reactive oxygen species (ROS), was increased in homozygous nrtp1-D mutant roots. These results indicate that, in addition to the mechanism of defense response common to both roots and shoots, a novel pathway may also exist in rice roots, which does not operate in shoots.
KW - Defense
KW - Mutation
KW - Necrotic
KW - NO
KW - Pathogen
KW - Rice
KW - Root
KW - ROS
UR - http://www.scopus.com/inward/record.url?scp=85054416608&partnerID=8YFLogxK
U2 - 10.1186/s12284-018-0250-1
DO - 10.1186/s12284-018-0250-1
M3 - Journal article
AN - SCOPUS:85054416608
SN - 1939-8425
VL - 11
JO - Rice
JF - Rice
IS - 1
M1 - 54
ER -