A novel fluorescein derivative furfuraldehyde fluorescein hydrazone (FFH) has been synthesized by reacting fluorescein hydrazide with furfuraldehyde and characterized by 1H NMR, 13C NMR, MS and elemental analysis. Addition of Cu2+ to the solution of FFH results in a rapid color change from colorless to yellow together with an obvious new band appeared at 502 nm in the UV-vis absorption spectra. This change is attributed to the spirocycle form of FFH opened via coordination with Cu2+ in a 1:1 stoichiometry and their association constant is determined as 6.1 × 104 L mol-1. Experimental results indicate that the FFH can provide a rapid, selective and sensitive response to Cu2+ with a linear dynamic range 6.6-330 μmol/L. Common interferent ions do not show any interference on the Cu2+ determination. It is anticipated that FFH can be a good candidate probe and has potential application for Cu2+ determination in aqueous solution.
|Number of pages||6|
|Journal||Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy|
|Publication status||Published - 25 Mar 2014|
Scopus Subject Areas
- Analytical Chemistry
- Atomic and Molecular Physics, and Optics