A quinolinyl antipyrine based fluorescence sensor for Zn2+ and its application in bioimaging

Qi Hua You; Pui Shan Chan; Wing Hong Chan; Sam C. K. Hau; Albert W. M. Lee; N. K. Mak; Thomas C. W. Mak; Ricky N. S. Wong

doi:10.1039/c2ra21736h

A quinolinyl antipyrine based fluorescence sensor for Zn²⁺ and its application in bioimaging

Qi Hua You
, Pui Shan Chan
, Wing Hong Chan^*
, Sam C. K. Hau
, Albert W. M. Lee
, N. K. Mak
, Thomas C. W. Mak
, Ricky N. S. Wong

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

90 Citations (Scopus)

Abstract

By incorporating 4-aminoantipyrine moiety onto 8-aminoquinoline with a suitable spacer, a highly selective and sensitive fluorescent Zn²⁺ sensor, QPA, was designed and constructed. In 25% ACN-HEPES buffer pH 7.0 solution, QPA exhibited 10.6-fold fluorescence enhancement at 500 nm upon addition of Zn²⁺. The limit of detection (LOD) was calculated to be 1.3 × 10^-7 M according to fluorescence titration. The 1:1 binding mode of the metal complex was established by combined UV-vis, fluorescence and HRMS spectroscopic method. The membrane permeability of QPA to living cells and bioimaging of Zn²⁺ are demonstrated.

Original language	English
Pages (from-to)	11078-11083
Number of pages	6
Journal	RSC Advances
Volume	2
Issue number	29
DOIs	https://doi.org/10.1039/c2ra21736h
Publication status	Published - 21 Nov 2012

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title = "A quinolinyl antipyrine based fluorescence sensor for Zn2+ and its application in bioimaging",

abstract = "By incorporating 4-aminoantipyrine moiety onto 8-aminoquinoline with a suitable spacer, a highly selective and sensitive fluorescent Zn2+ sensor, QPA, was designed and constructed. In 25\% ACN-HEPES buffer pH 7.0 solution, QPA exhibited 10.6-fold fluorescence enhancement at 500 nm upon addition of Zn2+. The limit of detection (LOD) was calculated to be 1.3 × 10-7 M according to fluorescence titration. The 1:1 binding mode of the metal complex was established by combined UV-vis, fluorescence and HRMS spectroscopic method. The membrane permeability of QPA to living cells and bioimaging of Zn2+ are demonstrated.",

author = "You, \{Qi Hua\} and Chan, \{Pui Shan\} and Chan, \{Wing Hong\} and Hau, \{Sam C. K.\} and Lee, \{Albert W. M.\} and Mak, \{N. K.\} and Mak, \{Thomas C. W.\} and Wong, \{Ricky N. S.\}",

note = "Funding information: The work was supported by a grant from the Research Committee of Hong Kong Baptist University (FRG2/11-12/121). Publisher copyright: {\textcopyright} The Royal Society of Chemistry 2012",

year = "2012",

month = nov,

day = "21",

doi = "10.1039/c2ra21736h",

language = "English",

volume = "2",

pages = "11078--11083",

journal = "RSC Advances",

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T1 - A quinolinyl antipyrine based fluorescence sensor for Zn2+ and its application in bioimaging

AU - You, Qi Hua

AU - Chan, Pui Shan

AU - Chan, Wing Hong

AU - Hau, Sam C. K.

AU - Lee, Albert W. M.

AU - Mak, N. K.

AU - Mak, Thomas C. W.

AU - Wong, Ricky N. S.

PY - 2012/11/21

Y1 - 2012/11/21

N2 - By incorporating 4-aminoantipyrine moiety onto 8-aminoquinoline with a suitable spacer, a highly selective and sensitive fluorescent Zn2+ sensor, QPA, was designed and constructed. In 25% ACN-HEPES buffer pH 7.0 solution, QPA exhibited 10.6-fold fluorescence enhancement at 500 nm upon addition of Zn2+. The limit of detection (LOD) was calculated to be 1.3 × 10-7 M according to fluorescence titration. The 1:1 binding mode of the metal complex was established by combined UV-vis, fluorescence and HRMS spectroscopic method. The membrane permeability of QPA to living cells and bioimaging of Zn2+ are demonstrated.

AB - By incorporating 4-aminoantipyrine moiety onto 8-aminoquinoline with a suitable spacer, a highly selective and sensitive fluorescent Zn2+ sensor, QPA, was designed and constructed. In 25% ACN-HEPES buffer pH 7.0 solution, QPA exhibited 10.6-fold fluorescence enhancement at 500 nm upon addition of Zn2+. The limit of detection (LOD) was calculated to be 1.3 × 10-7 M according to fluorescence titration. The 1:1 binding mode of the metal complex was established by combined UV-vis, fluorescence and HRMS spectroscopic method. The membrane permeability of QPA to living cells and bioimaging of Zn2+ are demonstrated.

UR - https://www.scopus.com/pages/publications/84871089522

U2 - 10.1039/c2ra21736h

DO - 10.1039/c2ra21736h

M3 - Journal article

AN - SCOPUS:84871089522

SN - 2046-2069

VL - 2

SP - 11078

EP - 11083

JO - RSC Advances

JF - RSC Advances

IS - 29

ER -

A quinolinyl antipyrine based fluorescence sensor for Zn²⁺ and its application in bioimaging

Abstract

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