A quencher-tether-ligand probe and its application in biosensor based on conjugated polymer

Dan Zhao; Juan Du; Yanguo Chen; Xinghu Ji; Zhike He; Wing Hong Chan

doi:10.1021/ma8000753

A quencher-tether-ligand probe and its application in biosensor based on conjugated polymer

Dan Zhao, Juan Du, Yanguo Chen, Xinghu Ji, Zhike He^*, Wing Hong Chan

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

32 Citations (Scopus)

Abstract

Water-soluble conjugated polymers (CPs) are a versatile class of advanced organic materials with excellent photochemistry properties. Using a biotin-modified fluorescence quencher (quencher-tether-ligand, QTL) to specifically sense the presence of avidin based on CP is of great importance. However, the nonspecific interactions between various proteins and CPs greatly baffle the studies of the mechanism and applications of CP-based biosensors. We selected luminescent ruthenium(II) polypyridine biotin complex (Ru-biotin) as a proper probe, which can not only work as a fluorescent quencher but also produces fluorescence resonance energy transfer (FRET) with anionic water-soluble CP (poly[5-methoxy-2-(3-sulfopropoxy)-1,4-phenylenecinylene] (MPS-PPV)). As the emission peak of Ru-biotin produced by FRET cannot be infected by nonspecific proteins, we discussed the MPS-PPV/Ru-biotin/avidin biosensing mechanism based on the fluorescence spectra changes of polymer and Ru-biotin and consequently realized the rapid and specific detection of avidin.

Original language	English
Pages (from-to)	5373-5378
Number of pages	6
Journal	Macromolecules
Volume	41
Issue number	14
DOIs	https://doi.org/10.1021/ma8000753
Publication status	Published - 22 Jul 2008

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title = "A quencher-tether-ligand probe and its application in biosensor based on conjugated polymer",

abstract = "Water-soluble conjugated polymers (CPs) are a versatile class of advanced organic materials with excellent photochemistry properties. Using a biotin-modified fluorescence quencher (quencher-tether-ligand, QTL) to specifically sense the presence of avidin based on CP is of great importance. However, the nonspecific interactions between various proteins and CPs greatly baffle the studies of the mechanism and applications of CP-based biosensors. We selected luminescent ruthenium(II) polypyridine biotin complex (Ru-biotin) as a proper probe, which can not only work as a fluorescent quencher but also produces fluorescence resonance energy transfer (FRET) with anionic water-soluble CP (poly[5-methoxy-2-(3-sulfopropoxy)-1,4-phenylenecinylene] (MPS-PPV)). As the emission peak of Ru-biotin produced by FRET cannot be infected by nonspecific proteins, we discussed the MPS-PPV/Ru-biotin/avidin biosensing mechanism based on the fluorescence spectra changes of polymer and Ru-biotin and consequently realized the rapid and specific detection of avidin.",

author = "Dan Zhao and Juan Du and Yanguo Chen and Xinghu Ji and Zhike He and Chan, {Wing Hong}",

note = "We thank Dr. Kenneth Kam-Wing Lo from City University of Hong Kong, People{\textquoteright}s Republic of China, for providing the complex Ru-biotin as a gift. This work was supported by the National Science Foundation of China (20575046, 90717111), the Science Fund for Creative Research Groups of NSFC (20621502),the Innovative Research Team in University (IRT0543), the National Key Scientific Program: Nanoscience and Nanotechnology (No. 2006CB933103), and 973 program (No 2007CB714507). ",

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T1 - A quencher-tether-ligand probe and its application in biosensor based on conjugated polymer

AU - Zhao, Dan

AU - Du, Juan

AU - Chen, Yanguo

AU - Ji, Xinghu

AU - He, Zhike

AU - Chan, Wing Hong

N1 - We thank Dr. Kenneth Kam-Wing Lo from City University of Hong Kong, People’s Republic of China, for providing the complex Ru-biotin as a gift. This work was supported by the National Science Foundation of China (20575046, 90717111), the Science Fund for Creative Research Groups of NSFC (20621502),the Innovative Research Team in University (IRT0543), the National Key Scientific Program: Nanoscience and Nanotechnology (No. 2006CB933103), and 973 program (No 2007CB714507).

PY - 2008/7/22

Y1 - 2008/7/22

N2 - Water-soluble conjugated polymers (CPs) are a versatile class of advanced organic materials with excellent photochemistry properties. Using a biotin-modified fluorescence quencher (quencher-tether-ligand, QTL) to specifically sense the presence of avidin based on CP is of great importance. However, the nonspecific interactions between various proteins and CPs greatly baffle the studies of the mechanism and applications of CP-based biosensors. We selected luminescent ruthenium(II) polypyridine biotin complex (Ru-biotin) as a proper probe, which can not only work as a fluorescent quencher but also produces fluorescence resonance energy transfer (FRET) with anionic water-soluble CP (poly[5-methoxy-2-(3-sulfopropoxy)-1,4-phenylenecinylene] (MPS-PPV)). As the emission peak of Ru-biotin produced by FRET cannot be infected by nonspecific proteins, we discussed the MPS-PPV/Ru-biotin/avidin biosensing mechanism based on the fluorescence spectra changes of polymer and Ru-biotin and consequently realized the rapid and specific detection of avidin.

AB - Water-soluble conjugated polymers (CPs) are a versatile class of advanced organic materials with excellent photochemistry properties. Using a biotin-modified fluorescence quencher (quencher-tether-ligand, QTL) to specifically sense the presence of avidin based on CP is of great importance. However, the nonspecific interactions between various proteins and CPs greatly baffle the studies of the mechanism and applications of CP-based biosensors. We selected luminescent ruthenium(II) polypyridine biotin complex (Ru-biotin) as a proper probe, which can not only work as a fluorescent quencher but also produces fluorescence resonance energy transfer (FRET) with anionic water-soluble CP (poly[5-methoxy-2-(3-sulfopropoxy)-1,4-phenylenecinylene] (MPS-PPV)). As the emission peak of Ru-biotin produced by FRET cannot be infected by nonspecific proteins, we discussed the MPS-PPV/Ru-biotin/avidin biosensing mechanism based on the fluorescence spectra changes of polymer and Ru-biotin and consequently realized the rapid and specific detection of avidin.

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A quencher-tether-ligand probe and its application in biosensor based on conjugated polymer

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