A prototypic system of parallel electrophoresis in multiple capillaries coupled with microwell arrays

Jing Su, Kangning REN, Wen Dai, Yihua Zhao, Jianhua Zhou, Hongkai Wu*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

1 Citation (Scopus)

Abstract

We present a microfluidic system that can be directly coupled with microwell array and perform parallel electrophoresis in multiple capillaries simultaneously. The system is based on an array of glass capillaries, fixed in a polydimethylsiloxane (PDMS) microfluidic scaffold, with one end open for interfacing with microwells. In this capillary array, every two adjacent capillaries act as a pair to be coupled with one microwell; samples in the microwells are introduced and separated by simply applying voltage between two electrodes that are placed at the other ends of capillaries; thus no complicated circuit design is required. We evaluate the performance of this system and perform multiple CE with direct sample introduction from microwell array. Also with this system, we demonstrate the analysis of cellular contents of cells lysed in a microwell array. Our results show that this prototypic system is a promising platform for high-throughput analysis of samples in microwell arrays.

Original languageEnglish
Pages (from-to)3324-3330
Number of pages7
JournalElectrophoresis
Volume32
Issue number23
DOIs
Publication statusPublished - Nov 2011

Scopus Subject Areas

  • Biochemistry
  • Clinical Biochemistry

User-Defined Keywords

  • Capillary array electrophoresis
  • High throughput
  • Microfluidics
  • Microwell array

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