A Probe Based on a Soft Salt Complex for Ratiometric and Lifetime Imaging of Variations in Intracellular Oxygen Content

Yun Ma; Yafang Dong; Liang Zou; Liang Shen; Suyi Liu; Shujuan Liu; Wei Huang; Qiang Zhao; Wai Yeung Wong

doi:10.1002/ejic.201800250

A Probe Based on a Soft Salt Complex for Ratiometric and Lifetime Imaging of Variations in Intracellular Oxygen Content

Yun Ma, Yafang Dong, Liang Zou, Liang Shen, Suyi Liu, Shujuan Liu, Wei Huang, Qiang Zhao^*, Wai Yeung Wong^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

11 Citations (Scopus)

Abstract

A novel soft salt based probe, which consists of a cationic zinc(II) complex and an anionic iridium(III) complex with two distinguishable emission colors, has been developed for the detection of oxygen content variations. Importantly, this soft salt based probe has been successfully utilized for ratiometric and lifetime imaging of intracellular oxygen content alterations.

In this study, a soft salt complex based probe has been exploited for measuring oxygen content changes in living cells. The zinc(II) complex [Zn(tpy)₂]²⁺(CH₃COO^–)₂ {tpy = 3-[(2,2′:6′,2′′-terpyridin)-4′-yl]-9-hexyl-9H-carbazole} was chosen as the cationic component, and the iridium(III) complex [Ir(tpq)₂CN₂]^–Bu₄N⁺ [tpq = 2-(thiophen-2-yl)quinoline] was selected as the anionic component. The soft salt complex based probe was formed through electrostatic interaction between two oppositely charged complexes. This probe exhibits ratiometric photoluminescence response to different oxygen concentrations with two separated emission bands. Furthermore, the application of this probe in ratiometric and lifetime imaging of oxygen content changes in living cells has been demonstrated.

Original language	English
Pages (from-to)	2345-2349
Number of pages	5
Journal	European Journal of Inorganic Chemistry
Volume	2018
Issue number	20-21
Early online date	25 Apr 2018
DOIs	https://doi.org/10.1002/ejic.201800250
Publication status	Published - 7 Jun 2018

Scopus Subject Areas

Inorganic Chemistry

User-Defined Keywords

Fluorescent probes
Imaging agents
Oxygen
Ratiometric imaging
Soft salt complexes

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10.1002/ejic.201800250

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title = "A Probe Based on a Soft Salt Complex for Ratiometric and Lifetime Imaging of Variations in Intracellular Oxygen Content",

abstract = "A novel soft salt based probe, which consists of a cationic zinc(II) complex and an anionic iridium(III) complex with two distinguishable emission colors, has been developed for the detection of oxygen content variations. Importantly, this soft salt based probe has been successfully utilized for ratiometric and lifetime imaging of intracellular oxygen content alterations.In this study, a soft salt complex based probe has been exploited for measuring oxygen content changes in living cells. The zinc(II) complex [Zn(tpy)2]2+(CH3COO–)2 {tpy = 3-[(2,2′:6′,2′′-terpyridin)-4′-yl]-9-hexyl-9H-carbazole} was chosen as the cationic component, and the iridium(III) complex [Ir(tpq)2CN2]–Bu4N+ [tpq = 2-(thiophen-2-yl)quinoline] was selected as the anionic component. The soft salt complex based probe was formed through electrostatic interaction between two oppositely charged complexes. This probe exhibits ratiometric photoluminescence response to different oxygen concentrations with two separated emission bands. Furthermore, the application of this probe in ratiometric and lifetime imaging of oxygen content changes in living cells has been demonstrated.",

keywords = "Fluorescent probes, Imaging agents, Oxygen, Ratiometric imaging, Soft salt complexes",

author = "Yun Ma and Yafang Dong and Liang Zou and Liang Shen and Suyi Liu and Shujuan Liu and Wei Huang and Qiang Zhao and Wong, {Wai Yeung}",

note = "Funding Information: We are grateful for financial support from the Hong Kong Research Grants Council (HKBU 12304715), the Hong Kong Polytechnic University (1-ZE1C), Ms Clarea Au for the Endowed Professorship (847S), the National Program for Support of Top-Notch Young Professionals, the National Natural Science Foundation of China (21701087 and 21671061), the Natural Science Foundation of Jiangsu Province of China (BK20160885), and Nanjing University of Posts and Telecommunications (NY216026).",

year = "2018",

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AU - Ma, Yun

AU - Dong, Yafang

AU - Zou, Liang

AU - Shen, Liang

AU - Liu, Suyi

AU - Liu, Shujuan

AU - Huang, Wei

AU - Zhao, Qiang

AU - Wong, Wai Yeung

N1 - Funding Information: We are grateful for financial support from the Hong Kong Research Grants Council (HKBU 12304715), the Hong Kong Polytechnic University (1-ZE1C), Ms Clarea Au for the Endowed Professorship (847S), the National Program for Support of Top-Notch Young Professionals, the National Natural Science Foundation of China (21701087 and 21671061), the Natural Science Foundation of Jiangsu Province of China (BK20160885), and Nanjing University of Posts and Telecommunications (NY216026).

PY - 2018/6/7

Y1 - 2018/6/7

N2 - A novel soft salt based probe, which consists of a cationic zinc(II) complex and an anionic iridium(III) complex with two distinguishable emission colors, has been developed for the detection of oxygen content variations. Importantly, this soft salt based probe has been successfully utilized for ratiometric and lifetime imaging of intracellular oxygen content alterations.In this study, a soft salt complex based probe has been exploited for measuring oxygen content changes in living cells. The zinc(II) complex [Zn(tpy)2]2+(CH3COO–)2 {tpy = 3-[(2,2′:6′,2′′-terpyridin)-4′-yl]-9-hexyl-9H-carbazole} was chosen as the cationic component, and the iridium(III) complex [Ir(tpq)2CN2]–Bu4N+ [tpq = 2-(thiophen-2-yl)quinoline] was selected as the anionic component. The soft salt complex based probe was formed through electrostatic interaction between two oppositely charged complexes. This probe exhibits ratiometric photoluminescence response to different oxygen concentrations with two separated emission bands. Furthermore, the application of this probe in ratiometric and lifetime imaging of oxygen content changes in living cells has been demonstrated.

AB - A novel soft salt based probe, which consists of a cationic zinc(II) complex and an anionic iridium(III) complex with two distinguishable emission colors, has been developed for the detection of oxygen content variations. Importantly, this soft salt based probe has been successfully utilized for ratiometric and lifetime imaging of intracellular oxygen content alterations.In this study, a soft salt complex based probe has been exploited for measuring oxygen content changes in living cells. The zinc(II) complex [Zn(tpy)2]2+(CH3COO–)2 {tpy = 3-[(2,2′:6′,2′′-terpyridin)-4′-yl]-9-hexyl-9H-carbazole} was chosen as the cationic component, and the iridium(III) complex [Ir(tpq)2CN2]–Bu4N+ [tpq = 2-(thiophen-2-yl)quinoline] was selected as the anionic component. The soft salt complex based probe was formed through electrostatic interaction between two oppositely charged complexes. This probe exhibits ratiometric photoluminescence response to different oxygen concentrations with two separated emission bands. Furthermore, the application of this probe in ratiometric and lifetime imaging of oxygen content changes in living cells has been demonstrated.

KW - Fluorescent probes

KW - Imaging agents

KW - Oxygen

KW - Ratiometric imaging

KW - Soft salt complexes

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DO - 10.1002/ejic.201800250

M3 - Journal article

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SN - 1434-1948

VL - 2018

SP - 2345

EP - 2349

JO - European Journal of Inorganic Chemistry

JF - European Journal of Inorganic Chemistry

IS - 20-21

ER -

A Probe Based on a Soft Salt Complex for Ratiometric and Lifetime Imaging of Variations in Intracellular Oxygen Content

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