A Probe Based on a Soft Salt Complex for Ratiometric and Lifetime Imaging of Variations in Intracellular Oxygen Content

Yun Ma, Yafang Dong, Liang Zou, Liang Shen, Suyi Liu, Shujuan Liu, Wei Huang, Qiang Zhao*, Wai Yeung Wong*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

A novel soft salt based probe, which consists of a cationic zinc(II) complex and an anionic iridium(III) complex with two distinguishable emission colors, has been developed for the detection of oxygen content variations. Importantly, this soft salt based probe has been successfully utilized for ratiometric and lifetime imaging of intracellular oxygen content alterations.

In this study, a soft salt complex based probe has been exploited for measuring oxygen content changes in living cells. The zinc(II) complex [Zn(tpy)2]2+(CH3COO)2 {tpy = 3-[(2,2′:6′,2′′-terpyridin)-4′-yl]-9-hexyl-9H-carbazole} was chosen as the cationic component, and the iridium(III) complex [Ir(tpq)2CN2]Bu4N+ [tpq = 2-(thiophen-2-yl)quinoline] was selected as the anionic component. The soft salt complex based probe was formed through electrostatic interaction between two oppositely charged complexes. This probe exhibits ratiometric photoluminescence response to different oxygen concentrations with two separated emission bands. Furthermore, the application of this probe in ratiometric and lifetime imaging of oxygen content changes in living cells has been demonstrated.

Original languageEnglish
Pages (from-to)2345-2349
Number of pages5
JournalEuropean Journal of Inorganic Chemistry
Volume2018
Issue number20-21
Early online date25 Apr 2018
DOIs
Publication statusPublished - 7 Jun 2018

Scopus Subject Areas

  • Inorganic Chemistry

User-Defined Keywords

  • Fluorescent probes
  • Imaging agents
  • Oxygen
  • Ratiometric imaging
  • Soft salt complexes

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