A Practical Measurement Technique for Marine Particle Analysis using Flow Cytometry and Real-time Image Processing

R. K. Y. Chan; K. M. Un; Y. Jin; T. Y. Yip; D. Wu; X. Z. Wang; M. X. He

A Practical Measurement Technique for Marine Particle Analysis using Flow Cytometry and Real-time Image Processing

R. K. Y. Chan, K. M. Un, Y. Jin, T. Y. Yip, D. Wu, X. Z. Wang, M. X. He

Department of Physics

Research output: Chapter in book/report/conference proceeding › Conference proceeding

Abstract

A newly developed system that combines flow cytometry with real-time image capture for phytoplankton analysis is described in this paper. The system has a free flow cytometer arrangement in which the measurement volume is defined by two intersecting laser beams. Water is pumped through a tube and the scattered light and fluorescence are picked up simultaneously for particle identification and size estimation. An intensified CCD (ICCD) camera is configured in the system to capture images of phytoplankton as they traverse through the measurement volume. The images are then processed for shape and size analysis. The data obtained from the image system is a subset of the data from the cytometer and is used to enhance the system's reliability for particle size measurements.

Original language	English
Title of host publication	Proceedings of the 4th Pacific Ocean Remote Sensing Conference
Pages	177-179
Number of pages	3
Publication status	Published - Jul 1998
Event	The 4th Pacific Ocean Remote Sensing Conference - Qingdao, China Duration: 28 Jul 1998 → 31 Jul 1998

Conference

Conference	The 4th Pacific Ocean Remote Sensing Conference
Country/Territory	China
City	Qingdao
Period	28/07/98 → 31/07/98

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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@inproceedings{86d4e47b97244000b8468b559f9883b9,

title = "A Practical Measurement Technique for Marine Particle Analysis using Flow Cytometry and Real-time Image Processing",

abstract = "A newly developed system that combines flow cytometry with real-time image capture for phytoplankton analysis is described in this paper. The system has a free flow cytometer arrangement in which the measurement volume is defined by two intersecting laser beams. Water is pumped through a tube and the scattered light and fluorescence are picked up simultaneously for particle identification and size estimation. An intensified CCD (ICCD) camera is configured in the system to capture images of phytoplankton as they traverse through the measurement volume. The images are then processed for shape and size analysis. The data obtained from the image system is a subset of the data from the cytometer and is used to enhance the system's reliability for particle size measurements.",

author = "Chan, {R. K. Y.} and Un, {K. M.} and Y. Jin and Yip, {T. Y.} and D. Wu and Wang, {X. Z.} and He, {M. X.}",

year = "1998",

month = jul,

language = "English",

pages = "177--179",

booktitle = "Proceedings of the 4th Pacific Ocean Remote Sensing Conference",

note = "The 4th Pacific Ocean Remote Sensing Conference ; Conference date: 28-07-1998 Through 31-07-1998",

}

TY - GEN

T1 - A Practical Measurement Technique for Marine Particle Analysis using Flow Cytometry and Real-time Image Processing

AU - Chan, R. K. Y.

AU - Un, K. M.

AU - Jin, Y.

AU - Yip, T. Y.

AU - Wu, D.

AU - Wang, X. Z.

AU - He, M. X.

PY - 1998/7

Y1 - 1998/7

N2 - A newly developed system that combines flow cytometry with real-time image capture for phytoplankton analysis is described in this paper. The system has a free flow cytometer arrangement in which the measurement volume is defined by two intersecting laser beams. Water is pumped through a tube and the scattered light and fluorescence are picked up simultaneously for particle identification and size estimation. An intensified CCD (ICCD) camera is configured in the system to capture images of phytoplankton as they traverse through the measurement volume. The images are then processed for shape and size analysis. The data obtained from the image system is a subset of the data from the cytometer and is used to enhance the system's reliability for particle size measurements.

AB - A newly developed system that combines flow cytometry with real-time image capture for phytoplankton analysis is described in this paper. The system has a free flow cytometer arrangement in which the measurement volume is defined by two intersecting laser beams. Water is pumped through a tube and the scattered light and fluorescence are picked up simultaneously for particle identification and size estimation. An intensified CCD (ICCD) camera is configured in the system to capture images of phytoplankton as they traverse through the measurement volume. The images are then processed for shape and size analysis. The data obtained from the image system is a subset of the data from the cytometer and is used to enhance the system's reliability for particle size measurements.

M3 - Conference proceeding

SP - 177

EP - 179

BT - Proceedings of the 4th Pacific Ocean Remote Sensing Conference

T2 - The 4th Pacific Ocean Remote Sensing Conference

Y2 - 28 July 1998 through 31 July 1998

ER -

A Practical Measurement Technique for Marine Particle Analysis using Flow Cytometry and Real-time Image Processing

Abstract

Conference

UN SDGs

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