TY - JOUR
T1 - A polysaccharide from native Curcuma kwangsiensis and its mechanism of reversing MDSC-induced suppressive function
AU - Jiang, Siliang
AU - Ma, Jing
AU - Li, Yixuan
AU - Lu, Bowen
AU - Du, Juan
AU - Xu, Jun
AU - Qin, Zhihai
AU - Ning, Tao
AU - Dong, Caixia
N1 - Funding information:
The NMR of sample nCKAP-2 was measured by Dr. Zang Shanshan and Liu Xuehui in Chinese Academy of Sciences Research Platform for Protein Sciences. This work was supported by the National Natural Science Foundation of China (81803682) and Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnosis (CTD2018-03).
Publisher copyright:
© 2022 Published by Elsevier Ltd.
PY - 2022/12/1
Y1 - 2022/12/1
N2 - A purified polysaccharide nCKAP-2 was prepared from Curcuma kwangsiensis and characterized. Structural analyses revealed that nCKAP-2 contains a high-branched arabinan composed of mono-substituted (O-5, 17.07 %) and di-substituted (O-2,5, 16.67 %) (1 → 3)-α-Araf residues. Bioactive test showed that nCKAP-2 significantly reversed the suppression function of myeloid-derived suppressor cells (MDSCs) on T cells. Further study revealed that treatment of MDSCs with nCKAP-2 could induce apoptotic cell death at the G0/G1 phase via the intrinsic pathway as suggested from the up-regulation of cleaved caspase 3 and 9, cleaved PARP, and Bax and the down-regulation of Bcl-xl. This apoptotic process was mainly mediated by the TLR4-NF-κB signaling pathway. Additionally, the down-regulation of ROS level of MDSCs after nCKAP-2 treatment involved in this process. Summarily, we explain how nCKAP-2 reverses the MDSC-induced suppressive function on T cells, and provide a scientific basis for the clinical application and development of C. kwangsiensis.
AB - A purified polysaccharide nCKAP-2 was prepared from Curcuma kwangsiensis and characterized. Structural analyses revealed that nCKAP-2 contains a high-branched arabinan composed of mono-substituted (O-5, 17.07 %) and di-substituted (O-2,5, 16.67 %) (1 → 3)-α-Araf residues. Bioactive test showed that nCKAP-2 significantly reversed the suppression function of myeloid-derived suppressor cells (MDSCs) on T cells. Further study revealed that treatment of MDSCs with nCKAP-2 could induce apoptotic cell death at the G0/G1 phase via the intrinsic pathway as suggested from the up-regulation of cleaved caspase 3 and 9, cleaved PARP, and Bax and the down-regulation of Bcl-xl. This apoptotic process was mainly mediated by the TLR4-NF-κB signaling pathway. Additionally, the down-regulation of ROS level of MDSCs after nCKAP-2 treatment involved in this process. Summarily, we explain how nCKAP-2 reverses the MDSC-induced suppressive function on T cells, and provide a scientific basis for the clinical application and development of C. kwangsiensis.
KW - Curcuma kwangsiensis
KW - Polysaccharide
KW - Myeloid-derived suppressor cells
KW - TLR4-NF-κB
UR - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85137049836&doi=10.1016%2fj.carbpol.2022.120020&partnerID=40&md5=8a5ae530183b10722867977a1cb261eb
U2 - 10.1016/j.carbpol.2022.120020
DO - 10.1016/j.carbpol.2022.120020
M3 - Journal article
SN - 0144-8617
VL - 297
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
M1 - 120020
ER -