TY - JOUR
T1 - A Plastid-Localized Pentatricopeptide Repeat Protein is Required for Both Pollen Development and Plant Growth in Rice
AU - Liu, Yu Jun
AU - Liu, Xuejiao
AU - Chen, Hao
AU - Zheng, Peng
AU - Wang, Wenyi
AU - Wang, Liangchao
AU - Zhang, Jianhua
AU - Tu, Jumin
N1 - Funding Information:
We gratefully thank Inhwan Hwang (Pohang University of Science and Technology, Korea) for providing F1-ATPase-γ: RFP fusion construct and Tsuyoshi Nakagawa (Shimane University, Japan) for the pGWB vectors. The thanks are also to Dr. Cuicui Zhang for her help in experiments. We also thank Prof. Bao-Cai Tan (Shandong University) for helpful suggestions in writing this paper. This work was supported by funds from the National Natural Science Foundation of China (Project no. 31501375) and China Postdoctoral Science Foundation Funded Project (Project no. 2016M590542).
PY - 2017/12/1
Y1 - 2017/12/1
N2 - Several mitochondrial-targeted pentatricopeptide repeat (PPR) proteins involved in pollen development have been reported to be fertility restorer (Rf) proteins. However, the roles of plastid-localized PPR proteins in plant male reproduction are poorly defined. Here, we described a plastid-localized PPR-SMR protein, OsPPR676, which is required for plant growth and pollen development in rice. In this study, OsPPR676 was confirmed to be an interacted protein with Osj10gBTF3, β-subunit of nascent polypeptide-associated complex (β-NAC), by bimolecular fluorescence complementation assays, indicating that both proteins are probably involved in the same regulatory pathway of pollen development. Compared with other chloroplast-rich tissues, OsPPR676 was only weakly expressed in anther, but in the Mei and YM stages of pollen development, its expression was relatively strong in the tapetum. Disruption of OsPPR676 resulted in growth retardation of plants and partial sterility of pollens. Phenotypic analysis of different osppr676 mutant lines implied that the SMR domain was not essential for the function of OsPPR676. We further demonstrated that OsPPR676 is essential for production of plastid atpB subunit, and then plays crucial roles in biosynthesis of fatty acids, carbohydrates, and other organic matters via affecting activity of ATP synthase.
AB - Several mitochondrial-targeted pentatricopeptide repeat (PPR) proteins involved in pollen development have been reported to be fertility restorer (Rf) proteins. However, the roles of plastid-localized PPR proteins in plant male reproduction are poorly defined. Here, we described a plastid-localized PPR-SMR protein, OsPPR676, which is required for plant growth and pollen development in rice. In this study, OsPPR676 was confirmed to be an interacted protein with Osj10gBTF3, β-subunit of nascent polypeptide-associated complex (β-NAC), by bimolecular fluorescence complementation assays, indicating that both proteins are probably involved in the same regulatory pathway of pollen development. Compared with other chloroplast-rich tissues, OsPPR676 was only weakly expressed in anther, but in the Mei and YM stages of pollen development, its expression was relatively strong in the tapetum. Disruption of OsPPR676 resulted in growth retardation of plants and partial sterility of pollens. Phenotypic analysis of different osppr676 mutant lines implied that the SMR domain was not essential for the function of OsPPR676. We further demonstrated that OsPPR676 is essential for production of plastid atpB subunit, and then plays crucial roles in biosynthesis of fatty acids, carbohydrates, and other organic matters via affecting activity of ATP synthase.
UR - http://www.scopus.com/inward/record.url?scp=85029291718&partnerID=8YFLogxK
U2 - 10.1038/s41598-017-10727-x
DO - 10.1038/s41598-017-10727-x
M3 - Journal article
C2 - 28904339
AN - SCOPUS:85029291718
SN - 2045-2322
VL - 7
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 11484
ER -