A plant-unique ESCRT component, FYVE4, regulates multivesicular endosome biogenesis and plant growth

Chuanliang Liu, Yonglun Zeng, Hongbo Li, Chao Yang, Wenjin Shen, Min Xu, Zhidan Xiao, Tongsheng Chen, Baiying Li, Wenhan Cao, Liwen Jiang, Marisa S. Otegui, Caiji Gao*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

20 Citations (Scopus)

Abstract

- During evolution, land plants generated unique proteins that participate in endosomal sorting and multivesicular endosome (MVE) biogenesis, many of them with specific phosphoinositide-binding capabilities. Nonetheless, the function of most plant phosphoinositide-binding proteins in endosomal trafficking remains elusive.
- Here, we analysed several Arabidopsis mutants lacking predicted phosphoinositide-binding proteins and first identified fyve4-1 as a mutant with a hypersensitive response to high-boron conditions and defects in degradative vacuolar sorting of membrane proteins such as the borate exporter BOR1-GFP.
- FYVE4 encodes a plant-unique, FYVE domain-containing protein that interacts with SNF7, a core component of ESCRT-III (Endosomal Sorting Complex Required for Transport III). FYVE4 affects the membrane association of the late-acting ESCRT components SNF7 and VPS4, and modulates the formation of intraluminal vesicles (ILVs) inside MVEs. The critical function of FYVE4 in the ESCRT pathway was further demonstrated by the strong genetic interactions with SNF7B and LIP5. Although the fyve4-1, snf7b and lip5 single mutants were viable, the fyve4-1 snf7b and fyve4-1 lip5 double mutants were seedling lethal, with strong defects in MVE biogenesis and vacuolar sorting of ubiquitinated membrane proteins.
- Taken together, we identified FYVE4 as a novel plant endosomal regulator, which functions in ESCRTing pathway to regulate MVE biogenesis.
Original languageEnglish
Pages (from-to)193-209
Number of pages17
JournalNew Phytologist
Volume231
DOIs
Publication statusPublished - Mar 2021

Scopus Subject Areas

  • Physiology
  • Plant Science

User-Defined Keywords

  • endosomal sorting
  • ESCRT
  • FYVE domain proteins
  • membrane proteins
  • multivesicular endosome
  • vacuolar degradation

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