A parallel G-quadruplex-selective luminescent probe for the detection of nanomolar calcium(II) ion

Hong Zhang He; Modi Wang; Daniel Shiu Hin Chan; Chung Hang Leung; Xuexia Lin; Jin Ming Lin; Edmond Dik Lung MA

doi:10.1016/j.ymeth.2013.07.021

A parallel G-quadruplex-selective luminescent probe for the detection of nanomolar calcium(II) ion

Hong Zhang He, Modi Wang, Daniel Shiu Hin Chan, Chung Hang Leung, Xuexia Lin, Jin Ming Lin, Edmond Dik Lung MA^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

26 Citations (Scopus)

Abstract

A parallel G-quadruplex-selective iridium(III) complex has been synthesized and employed as a luminescent probe in a label-free G-quadruplex-based detection assay for Ca²⁺ ions in aqueous solution. In this assay, a guanine-rich oligonucleotide (G4, 5'-G₄T₄G₄-3') initially exists in an antiparallel G-quadruplex conformation, resulting in a low luminescence signal. Upon incubation with Ca²⁺ ions, the antiparallel G-quadruplex is induced into a parallel G-quadruplex conformation, which greatly enhances the luminescence emission of the iridium(III) probe. This method was highly sensitive for Ca²⁺ ions with a limit of detection in the nanomolar range, and was selective for Ca²⁺ over other metal ions.

Original language	English
Pages (from-to)	212-217
Number of pages	6
Journal	Methods
Volume	64
Issue number	3
DOIs	https://doi.org/10.1016/j.ymeth.2013.07.021
Publication status	Published - 15 Dec 2013

Scopus Subject Areas

Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)

User-Defined Keywords

G-quadruplex
Iridium
Luminescence
Metal complex

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10.1016/j.ymeth.2013.07.021

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title = "A parallel G-quadruplex-selective luminescent probe for the detection of nanomolar calcium(II) ion",

abstract = "A parallel G-quadruplex-selective iridium(III) complex has been synthesized and employed as a luminescent probe in a label-free G-quadruplex-based detection assay for Ca2+ ions in aqueous solution. In this assay, a guanine-rich oligonucleotide (G4, 5'-G4T4G4-3') initially exists in an antiparallel G-quadruplex conformation, resulting in a low luminescence signal. Upon incubation with Ca2+ ions, the antiparallel G-quadruplex is induced into a parallel G-quadruplex conformation, which greatly enhances the luminescence emission of the iridium(III) probe. This method was highly sensitive for Ca2+ ions with a limit of detection in the nanomolar range, and was selective for Ca2+ over other metal ions.",

keywords = "G-quadruplex, Iridium, Luminescence, Metal complex",

author = "He, {Hong Zhang} and Modi Wang and Chan, {Daniel Shiu Hin} and Leung, {Chung Hang} and Xuexia Lin and Lin, {Jin Ming} and MA, {Edmond Dik Lung}",

note = "Funding Information: This work is supported by Hong Kong Baptist University ( FRG2/11-12/009 and FRG2/12-13/021 ), Centre for Cancer and Inflammation Research, School of Chinese Medicine (CCIR-SCM, HKBU), the Health and Medical Research Fund (HMRF/11101212), the Research Grants Council (HKBU/201811, HKBU/204612 and HKBU/201913), the French National Research Agency/Research Grants Council Joint Research Scheme (A-HKBU201/12), the Science and Technology Development Fund, Macao SAR (001/2012/A) and the University of Macau (MYRG091(Y2-L2)-ICMS12-LCH and MYRG121(Y2-L2)-ICMS12-LCH). ",

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AU - He, Hong Zhang

AU - Wang, Modi

AU - Chan, Daniel Shiu Hin

AU - Leung, Chung Hang

AU - Lin, Xuexia

AU - Lin, Jin Ming

AU - MA, Edmond Dik Lung

N1 - Funding Information: This work is supported by Hong Kong Baptist University ( FRG2/11-12/009 and FRG2/12-13/021 ), Centre for Cancer and Inflammation Research, School of Chinese Medicine (CCIR-SCM, HKBU), the Health and Medical Research Fund (HMRF/11101212), the Research Grants Council (HKBU/201811, HKBU/204612 and HKBU/201913), the French National Research Agency/Research Grants Council Joint Research Scheme (A-HKBU201/12), the Science and Technology Development Fund, Macao SAR (001/2012/A) and the University of Macau (MYRG091(Y2-L2)-ICMS12-LCH and MYRG121(Y2-L2)-ICMS12-LCH).

PY - 2013/12/15

Y1 - 2013/12/15

N2 - A parallel G-quadruplex-selective iridium(III) complex has been synthesized and employed as a luminescent probe in a label-free G-quadruplex-based detection assay for Ca2+ ions in aqueous solution. In this assay, a guanine-rich oligonucleotide (G4, 5'-G4T4G4-3') initially exists in an antiparallel G-quadruplex conformation, resulting in a low luminescence signal. Upon incubation with Ca2+ ions, the antiparallel G-quadruplex is induced into a parallel G-quadruplex conformation, which greatly enhances the luminescence emission of the iridium(III) probe. This method was highly sensitive for Ca2+ ions with a limit of detection in the nanomolar range, and was selective for Ca2+ over other metal ions.

AB - A parallel G-quadruplex-selective iridium(III) complex has been synthesized and employed as a luminescent probe in a label-free G-quadruplex-based detection assay for Ca2+ ions in aqueous solution. In this assay, a guanine-rich oligonucleotide (G4, 5'-G4T4G4-3') initially exists in an antiparallel G-quadruplex conformation, resulting in a low luminescence signal. Upon incubation with Ca2+ ions, the antiparallel G-quadruplex is induced into a parallel G-quadruplex conformation, which greatly enhances the luminescence emission of the iridium(III) probe. This method was highly sensitive for Ca2+ ions with a limit of detection in the nanomolar range, and was selective for Ca2+ over other metal ions.

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A parallel G-quadruplex-selective luminescent probe for the detection of nanomolar calcium(II) ion

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