A parallel G-quadruplex-selective luminescent probe for the detection of nanomolar calcium(II) ion

Hong Zhang He; Modi Wang; Daniel Shiu Hin Chan; Chung Hang Leung; Xuexia Lin; Jin Ming Lin; Dik Lung Ma

doi:10.1016/j.ymeth.2013.07.021

A parallel G-quadruplex-selective luminescent probe for the detection of nanomolar calcium(II) ion

Hong Zhang He, Modi Wang, Daniel Shiu Hin Chan, Chung Hang Leung, Xuexia Lin, Jin Ming Lin, Dik Lung Ma^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

28 Citations (Scopus)

Abstract

A parallel G-quadruplex-selective iridium(III) complex has been synthesized and employed as a luminescent probe in a label-free G-quadruplex-based detection assay for Ca²⁺ ions in aqueous solution. In this assay, a guanine-rich oligonucleotide (G4, 5'-G₄T₄G₄-3') initially exists in an antiparallel G-quadruplex conformation, resulting in a low luminescence signal. Upon incubation with Ca²⁺ ions, the antiparallel G-quadruplex is induced into a parallel G-quadruplex conformation, which greatly enhances the luminescence emission of the iridium(III) probe. This method was highly sensitive for Ca²⁺ ions with a limit of detection in the nanomolar range, and was selective for Ca²⁺ over other metal ions.

Original language	English
Pages (from-to)	212-217
Number of pages	6
Journal	Methods
Volume	64
Issue number	3
DOIs	https://doi.org/10.1016/j.ymeth.2013.07.021
Publication status	Published - 15 Dec 2013

Scopus Subject Areas

Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)

User-Defined Keywords

G-quadruplex
Iridium
Luminescence
Metal complex

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10.1016/j.ymeth.2013.07.021

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title = "A parallel G-quadruplex-selective luminescent probe for the detection of nanomolar calcium(II) ion",

abstract = "A parallel G-quadruplex-selective iridium(III) complex has been synthesized and employed as a luminescent probe in a label-free G-quadruplex-based detection assay for Ca2+ ions in aqueous solution. In this assay, a guanine-rich oligonucleotide (G4, 5'-G4T4G4-3') initially exists in an antiparallel G-quadruplex conformation, resulting in a low luminescence signal. Upon incubation with Ca2+ ions, the antiparallel G-quadruplex is induced into a parallel G-quadruplex conformation, which greatly enhances the luminescence emission of the iridium(III) probe. This method was highly sensitive for Ca2+ ions with a limit of detection in the nanomolar range, and was selective for Ca2+ over other metal ions.",

keywords = "G-quadruplex, Iridium, Luminescence, Metal complex",

author = "He, {Hong Zhang} and Modi Wang and Chan, {Daniel Shiu Hin} and Leung, {Chung Hang} and Xuexia Lin and Lin, {Jin Ming} and Ma, {Dik Lung}",

note = "Funding Information: This work is supported by Hong Kong Baptist University ( FRG2/11-12/009 and FRG2/12-13/021 ), Centre for Cancer and Inflammation Research, School of Chinese Medicine (CCIR-SCM, HKBU), the Health and Medical Research Fund (HMRF/11101212), the Research Grants Council (HKBU/201811, HKBU/204612 and HKBU/201913), the French National Research Agency/Research Grants Council Joint Research Scheme (A-HKBU201/12), the Science and Technology Development Fund, Macao SAR (001/2012/A) and the University of Macau (MYRG091(Y2-L2)-ICMS12-LCH and MYRG121(Y2-L2)-ICMS12-LCH). ",

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AU - He, Hong Zhang

AU - Wang, Modi

AU - Chan, Daniel Shiu Hin

AU - Leung, Chung Hang

AU - Lin, Xuexia

AU - Lin, Jin Ming

AU - Ma, Dik Lung

N1 - Funding Information: This work is supported by Hong Kong Baptist University ( FRG2/11-12/009 and FRG2/12-13/021 ), Centre for Cancer and Inflammation Research, School of Chinese Medicine (CCIR-SCM, HKBU), the Health and Medical Research Fund (HMRF/11101212), the Research Grants Council (HKBU/201811, HKBU/204612 and HKBU/201913), the French National Research Agency/Research Grants Council Joint Research Scheme (A-HKBU201/12), the Science and Technology Development Fund, Macao SAR (001/2012/A) and the University of Macau (MYRG091(Y2-L2)-ICMS12-LCH and MYRG121(Y2-L2)-ICMS12-LCH).

PY - 2013/12/15

Y1 - 2013/12/15

N2 - A parallel G-quadruplex-selective iridium(III) complex has been synthesized and employed as a luminescent probe in a label-free G-quadruplex-based detection assay for Ca2+ ions in aqueous solution. In this assay, a guanine-rich oligonucleotide (G4, 5'-G4T4G4-3') initially exists in an antiparallel G-quadruplex conformation, resulting in a low luminescence signal. Upon incubation with Ca2+ ions, the antiparallel G-quadruplex is induced into a parallel G-quadruplex conformation, which greatly enhances the luminescence emission of the iridium(III) probe. This method was highly sensitive for Ca2+ ions with a limit of detection in the nanomolar range, and was selective for Ca2+ over other metal ions.

AB - A parallel G-quadruplex-selective iridium(III) complex has been synthesized and employed as a luminescent probe in a label-free G-quadruplex-based detection assay for Ca2+ ions in aqueous solution. In this assay, a guanine-rich oligonucleotide (G4, 5'-G4T4G4-3') initially exists in an antiparallel G-quadruplex conformation, resulting in a low luminescence signal. Upon incubation with Ca2+ ions, the antiparallel G-quadruplex is induced into a parallel G-quadruplex conformation, which greatly enhances the luminescence emission of the iridium(III) probe. This method was highly sensitive for Ca2+ ions with a limit of detection in the nanomolar range, and was selective for Ca2+ over other metal ions.

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A parallel G-quadruplex-selective luminescent probe for the detection of nanomolar calcium(II) ion

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