A parallel G-quadruplex-selective luminescent probe for the detection of nanomolar calcium(II) ion

Hong Zhang He, Modi Wang, Daniel Shiu Hin Chan, Chung Hang Leung, Xuexia Lin, Jin Ming Lin, Edmond Dik Lung MA*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

A parallel G-quadruplex-selective iridium(III) complex has been synthesized and employed as a luminescent probe in a label-free G-quadruplex-based detection assay for Ca2+ ions in aqueous solution. In this assay, a guanine-rich oligonucleotide (G4, 5'-G4T4G4-3') initially exists in an antiparallel G-quadruplex conformation, resulting in a low luminescence signal. Upon incubation with Ca2+ ions, the antiparallel G-quadruplex is induced into a parallel G-quadruplex conformation, which greatly enhances the luminescence emission of the iridium(III) probe. This method was highly sensitive for Ca2+ ions with a limit of detection in the nanomolar range, and was selective for Ca2+ over other metal ions.

Original languageEnglish
Pages (from-to)212-217
Number of pages6
JournalMethods
Volume64
Issue number3
DOIs
Publication statusPublished - 15 Dec 2013

Scopus Subject Areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

User-Defined Keywords

  • G-quadruplex
  • Iridium
  • Luminescence
  • Metal complex

Fingerprint

Dive into the research topics of 'A parallel G-quadruplex-selective luminescent probe for the detection of nanomolar calcium(II) ion'. Together they form a unique fingerprint.

Cite this