TY - JOUR
T1 - A novel inulin-type fructan from Asparagus cochinchinensis and its beneficial impact on human intestinal microbiota
AU - Sun, Qili
AU - Zhu, Lixia
AU - Li, Yixuan
AU - Cui, Yongsheng
AU - Jiang, Siliang
AU - Tao, Ning
AU - Chen, Hubiao
AU - Zhao, Zhongzhen
AU - Xu, Jun
AU - Dong, Caixia
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China ( 81803682 and 81872975 ), Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnosis ( CTD2018-03 ), and Hong Kong General Research Fund ( 12100219 ).
PY - 2020/11/1
Y1 - 2020/11/1
N2 - A purified inulin-type fructan named ACNP (Asparagus cochinchinensis neutral polysaccharide) with apparent molecular weight of 2690 Da was obtained from Asparagus cochinchinensis (Lour.) Merr. by ion-exchange and gel-filtration column chromatography. Structural analyses reveal that ACNP has a linear backbone composed of 2,1-β-D-Fruf residues, ending with a (1→2) bonded α-D-Glcp. The impacts of ACNP on gut microbiota were then investigated by in vitro fermentation with human fecal cultures. The results showed that ACNP was digested by gut microbiota, while the pH value in the fecal culture of ACNP was greatly decreased, and total short-chain fatty acids, acetic, propionic, i-valeric and n-valeric acids were significantly increased. Moreover, ACNP regulated the fecal microbiota composition by stimulating the growth of Prevotella, Megamonas, and Bifidobacterium while depleting Haemophilus. Collectively, these results indicated that ACNP beneficially regulates gut microbiota, which thus suggested that ACNP has the potential to be used as a dietary supplement or drug to improve health.
AB - A purified inulin-type fructan named ACNP (Asparagus cochinchinensis neutral polysaccharide) with apparent molecular weight of 2690 Da was obtained from Asparagus cochinchinensis (Lour.) Merr. by ion-exchange and gel-filtration column chromatography. Structural analyses reveal that ACNP has a linear backbone composed of 2,1-β-D-Fruf residues, ending with a (1→2) bonded α-D-Glcp. The impacts of ACNP on gut microbiota were then investigated by in vitro fermentation with human fecal cultures. The results showed that ACNP was digested by gut microbiota, while the pH value in the fecal culture of ACNP was greatly decreased, and total short-chain fatty acids, acetic, propionic, i-valeric and n-valeric acids were significantly increased. Moreover, ACNP regulated the fecal microbiota composition by stimulating the growth of Prevotella, Megamonas, and Bifidobacterium while depleting Haemophilus. Collectively, these results indicated that ACNP beneficially regulates gut microbiota, which thus suggested that ACNP has the potential to be used as a dietary supplement or drug to improve health.
KW - Asparagus cochinchinensis (Lour.) Merr.
KW - Gut microbiota
KW - In vitro fermentation
KW - Inulin-type fructan
KW - Short-chain fatty acids
UR - https://doi.org/10.1016/j.carbpol.2021.117748
UR - http://www.scopus.com/inward/record.url?scp=85087902959&partnerID=8YFLogxK
U2 - 10.1016/j.carbpol.2020.116761
DO - 10.1016/j.carbpol.2020.116761
M3 - Journal article
C2 - 32829873
AN - SCOPUS:85087902959
SN - 0144-8617
VL - 247
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
M1 - 116761
ER -
