A novel inulin-type fructan from Asparagus cochinchinensis and its beneficial impact on human intestinal microbiota

Qili Sun, Lixia Zhu, Yixuan Li, Yongsheng Cui, Siliang Jiang, Ning Tao, Hubiao CHEN, Zhongzhen ZHAO, Jun XU*, Caixia Dong

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

A purified inulin-type fructan named ACNP (Asparagus cochinchinensis neutral polysaccharide) with apparent molecular weight of 2690 Da was obtained from Asparagus cochinchinensis (Lour.) Merr. by ion-exchange and gel-filtration column chromatography. Structural analyses reveal that ACNP has a linear backbone composed of 2,1-β-D-Fruf residues, ending with a (1→2) bonded α-D-Glcp. The impacts of ACNP on gut microbiota were then investigated by in vitro fermentation with human fecal cultures. The results showed that ACNP was digested by gut microbiota, while the pH value in the fecal culture of ACNP was greatly decreased, and total short-chain fatty acids, acetic, propionic, i-valeric and n-valeric acids were significantly increased. Moreover, ACNP regulated the fecal microbiota composition by stimulating the growth of Prevotella, Megamonas, and Bifidobacterium while depleting Haemophilus. Collectively, these results indicated that ACNP beneficially regulates gut microbiota, which thus suggested that ACNP has the potential to be used as a dietary supplement or drug to improve health.

Original languageEnglish
Article number116761
JournalCarbohydrate Polymers
Volume247
DOIs
Publication statusPublished - 1 Nov 2020

Scopus Subject Areas

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

User-Defined Keywords

  • Asparagus cochinchinensis (Lour.) Merr.
  • Gut microbiota
  • In vitro fermentation
  • Inulin-type fructan
  • Short-chain fatty acids

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