A novel chemosensor for the distinguishable detections of Cu²⁺ and Hg²⁺ by off–on fluorescence and ratiometric UV–visible absorption

A novel dual-functional chemosensor, derived from the conjugation of rhodamine B with a quinoline derivative (RHQ), was firstly synthesized with high efficiency and cost-effectiveness for the distinguishable detections of Cu²⁺ and Hg²⁺ via ring-opening and ring-forming mechanism. The chemosensor exhibits highly selective and distinguishable responses for Cu²⁺ and Hg²⁺ in CH₃CN–H₂O (4:1, v/v) with off–on fluorescence and ratiometric ultraviolet–visible (UV–Vis) absorption changes. Additionally, Cu²⁺ is identified by opening a rhodamine spirocycle with a UV–Vis absorption band, at around 560 nm and fluorescence turn-on. Interestingly, Hg²⁺ is discerned by opening the rhodamine spirocycle and by generating a new special cycle for the quinoline unit. Resultantly, there were two UV–Vis absorption bands at around 365 nm and 560 nm, which were accompanied by fluorescence turn-on. Moreover, the chemosensor can quantitatively detect Cu²⁺ and Hg²⁺ by off–on fluorescence and ratiometric UV–Vis absorption changes, respectively. Furthermore, the chemosensor with low cytotoxicity could be successfully administered to monitor Cu²⁺ and Hg²⁺ in living cells. This work may pay the way for the development of dual-functional chemosensor for quantificationally detecting metal ions in environmental and biological systems.