TY - JOUR
T1 - A novel and rapid HPGPC-based strategy for quality control of saccharide-dominant herbal materials
T2 - Dendrobium officinale, a case study
AU - Xu, Jun
AU - Li, Song-Lin
AU - Yue, Rui-Qi
AU - Ko, Chun-Hay
AU - Hu, Jiang-Miao
AU - Liu, Jing
AU - Ho, Hing-Man
AU - Yi, Tao
AU - Zhao, Zhong-Zhen
AU - Zhou, Jun
AU - Leung, Ping-Chung
AU - Chen, Hu-Biao
AU - Han, Quan-Bin
N1 - Funding Information:
Acknowledgments This study was funded by Hong Kong Baptist University (FRG2/11-12/048, FRG1/12-13/018, FRG2/12-13/006, FRG2/13-14/026, MPCF-001-2014/2015, and RC-start up grant).
PY - 2014/10
Y1 - 2014/10
N2 - Qualitative and quantitative characterization of natural
saccharides, especially polysaccharides, in herb materials remains a
challenge due to their complicated structures and high macromolecular
masses. Currently available methods involve time-consuming and
complicated operations, and present poor specificity. Here, a novel and
rapid high-performance gel permeation chromatography (HPGPC)-based
approach is described for quality assessment of saccharide-dominant
herbal materials by simultaneous qualitative and quantitative analysis
of saccharide components. Dendrobium officinale, one of
the rarest tonic herbs worldwide, was employed as the model herb in
this study. First, a HPGPC fingerprint based on the molecular weight
distribution of its carbohydrate components was established for
qualitative identification of D. officinale. Then,
HPGPC-guided dominant holistic polysaccharide marker was separated using
ultra-filtration followed by HPGPC determination for quantitative
evaluation of D. officinale. The experimental results
suggest that this method is more efficient, stable, and convenient
compared with the currently available methods for authentication and
quality evaluation of D. officinale, and we expect the
method will have similar advantages when used for quality control of
other saccharide-dominant herbal materials and products.
AB - Qualitative and quantitative characterization of natural
saccharides, especially polysaccharides, in herb materials remains a
challenge due to their complicated structures and high macromolecular
masses. Currently available methods involve time-consuming and
complicated operations, and present poor specificity. Here, a novel and
rapid high-performance gel permeation chromatography (HPGPC)-based
approach is described for quality assessment of saccharide-dominant
herbal materials by simultaneous qualitative and quantitative analysis
of saccharide components. Dendrobium officinale, one of
the rarest tonic herbs worldwide, was employed as the model herb in
this study. First, a HPGPC fingerprint based on the molecular weight
distribution of its carbohydrate components was established for
qualitative identification of D. officinale. Then,
HPGPC-guided dominant holistic polysaccharide marker was separated using
ultra-filtration followed by HPGPC determination for quantitative
evaluation of D. officinale. The experimental results
suggest that this method is more efficient, stable, and convenient
compared with the currently available methods for authentication and
quality evaluation of D. officinale, and we expect the
method will have similar advantages when used for quality control of
other saccharide-dominant herbal materials and products.
KW - HPGPC fingerprints
KW - Saccharide-dominantherbal materials
KW - Holistic polysaccharide marker
KW - Quality evaluation
KW - Dendrobium officinale
UR - http://www.scopus.com/inward/record.url?scp=84920706260&partnerID=8YFLogxK
U2 - 10.1007/s00216-014-8060-9
DO - 10.1007/s00216-014-8060-9
M3 - Journal article
C2 - 25106542
AN - SCOPUS:84920706260
SN - 1618-2642
VL - 406
SP - 6409
EP - 6417
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
IS - 25
ER -