A New Self-Assembling System for Targeted Gene Delivery

Michael A.W. Eaton; Terence S. Baker; Catherine F. Catterall; Kenneth Crook; Graham S. Macaulay; Barbara Mason; Timothy J. Norman; David Parker; Justin J.B. Perry; Richard J. Taylor; Alison Turner; A. Neil Weir

doi:10.1002/1521-3773(20001117)39:22<4063::AID-ANIE4063>3.0.CO;2-X

A New Self-Assembling System for Targeted Gene Delivery

Michael A.W. Eaton^*
, Terence S. Baker
, Catherine F. Catterall
, Kenneth Crook
, Graham S. Macaulay
, Barbara Mason
, Timothy J. Norman
, David Parker^*
, Justin J.B. Perry
, Richard J. Taylor
, Alison Turner
, A. Neil Weir

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

19 Citations (Scopus)

Abstract

Polyamine-containing bolaamphiphiles bind and compact DNA to form well-defined assemblies that resist nuclease attack and efficiently transfect Chinese hamster ovary cells. Nonspecific uptake is reduced by the incorporation of a low percentage of polyethylene glycol bolaamphiphile and the targeted transfection of a human T cell line has been demonstrated using an internalizing anti-CD3-antibody/bolaamphiphile conjugate incorporated at 0.004 % into the supramolecular array.

Original language	English
Pages (from-to)	4063-4067
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	39
Issue number	22
Early online date	14 Nov 2000
DOIs	https://doi.org/10.1002/1521-3773(20001117)39:22<4063::AID-ANIE4063>3.0.CO;2-X
Publication status	Published - 17 Nov 2000

User-Defined Keywords

Amphiphiles
Antigens
DNA recognition
Gene technology
Supramolecular chemistry

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10.1002/1521-3773(20001117)39:22<4063::AID-ANIE4063>3.0.CO;2-X

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Eaton, M. A. W., Baker, T. S., Catterall, C. F., Crook, K., Macaulay, G. S., Mason, B., Norman, T. J., Parker, D., Perry, J. J. B., Taylor, R. J., Turner, A., & Weir, A. N. (2000). A New Self-Assembling System for Targeted Gene Delivery. Angewandte Chemie - International Edition, 39(22), 4063-4067. https://doi.org/10.1002/1521-3773(20001117)39:22<4063::AID-ANIE4063>3.0.CO;2-X

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title = "A New Self-Assembling System for Targeted Gene Delivery",

abstract = "Polyamine-containing bolaamphiphiles bind and compact DNA to form well-defined assemblies that resist nuclease attack and efficiently transfect Chinese hamster ovary cells. Nonspecific uptake is reduced by the incorporation of a low percentage of polyethylene glycol bolaamphiphile and the targeted transfection of a human T cell line has been demonstrated using an internalizing anti-CD3-antibody/bolaamphiphile conjugate incorporated at 0.004 \% into the supramolecular array.",

keywords = "Amphiphiles, Antigens, DNA recognition, Gene technology, Supramolecular chemistry",

author = "Eaton, \{Michael A.W.\} and Baker, \{Terence S.\} and Catterall, \{Catherine F.\} and Kenneth Crook and Macaulay, \{Graham S.\} and Barbara Mason and Norman, \{Timothy J.\} and David Parker and Perry, \{Justin J.B.\} and Taylor, \{Richard J.\} and Alison Turner and Weir, \{A. Neil\}",

note = " Publisher Copyright: {\textcopyright} WILEY-VCH Verlag GmbH, D-69451 Weinheim, 2000.",

year = "2000",

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doi = "10.1002/1521-3773(20001117)39:22<4063::AID-ANIE4063>3.0.CO;2-X",

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T1 - A New Self-Assembling System for Targeted Gene Delivery

AU - Eaton, Michael A.W.

AU - Baker, Terence S.

AU - Catterall, Catherine F.

AU - Crook, Kenneth

AU - Macaulay, Graham S.

AU - Mason, Barbara

AU - Norman, Timothy J.

AU - Parker, David

AU - Perry, Justin J.B.

AU - Taylor, Richard J.

AU - Turner, Alison

AU - Weir, A. Neil

PY - 2000/11/17

Y1 - 2000/11/17

N2 - Polyamine-containing bolaamphiphiles bind and compact DNA to form well-defined assemblies that resist nuclease attack and efficiently transfect Chinese hamster ovary cells. Nonspecific uptake is reduced by the incorporation of a low percentage of polyethylene glycol bolaamphiphile and the targeted transfection of a human T cell line has been demonstrated using an internalizing anti-CD3-antibody/bolaamphiphile conjugate incorporated at 0.004 % into the supramolecular array.

AB - Polyamine-containing bolaamphiphiles bind and compact DNA to form well-defined assemblies that resist nuclease attack and efficiently transfect Chinese hamster ovary cells. Nonspecific uptake is reduced by the incorporation of a low percentage of polyethylene glycol bolaamphiphile and the targeted transfection of a human T cell line has been demonstrated using an internalizing anti-CD3-antibody/bolaamphiphile conjugate incorporated at 0.004 % into the supramolecular array.

KW - Amphiphiles

KW - Antigens

KW - DNA recognition

KW - Gene technology

KW - Supramolecular chemistry

UR - https://www.scopus.com/pages/publications/0034680623

U2 - 10.1002/1521-3773(20001117)39:22<4063::AID-ANIE4063>3.0.CO;2-X

DO - 10.1002/1521-3773(20001117)39:22<4063::AID-ANIE4063>3.0.CO;2-X

M3 - Journal article

AN - SCOPUS:0034680623

SN - 1433-7851

VL - 39

SP - 4063

EP - 4067

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 22

ER -

A New Self-Assembling System for Targeted Gene Delivery

Abstract

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