A new peptide-based chemosensor for selective imaging of copper ion and hydrogen sulfide in living cells

Chaowei Hao; Yongxin Li; Baomin Fan; Guangzhi Zeng; Danni Zhang; Zhaoxiang Bian; Jiang Wu

doi:10.1016/j.microc.2020.104658

A new peptide-based chemosensor for selective imaging of copper ion and hydrogen sulfide in living cells

Chaowei Hao, Yongxin Li, Baomin Fan, Guangzhi Zeng, Danni Zhang, Zhaoxiang Bian^*, Jiang Wu^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

14 Citations (Scopus)

Abstract

Hydrogen sulfide (H₂S), like CO and NO, is an endogenous gas signal molecule that can play a vital role in many physiological processes. Therefore, it's of great significance to study the efficient and sensitive imaging technology of H₂S in vivo. Copper is an essential trace element in the human body that is very important for many cellular functions and related metabolic processes. In this study we report the design of a peptide L (FITC-Ahx-His-Glu-Phe-His-NH₂) that can act as an “On-Off-On” fluorescent chemical sensor to detect Cu²⁺ and S²⁻, and cysteine (Cys) and glutathione (GSH) can also replace Cu²⁺ from L-Cu system. The capability of this peptide fluorescent probe to detect H₂S in HeLa cells is also evaluated.

Original language	English
Article number	104658
Journal	Microchemical Journal
Volume	154
DOIs	https://doi.org/10.1016/j.microc.2020.104658
Publication status	Published - May 2020

Scopus Subject Areas

Analytical Chemistry
Spectroscopy

User-Defined Keywords

Cells imaging
Copper ion detection
Hydrogen sulfide detection
Peptide-based

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10.1016/j.microc.2020.104658

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title = "A new peptide-based chemosensor for selective imaging of copper ion and hydrogen sulfide in living cells",

abstract = "Hydrogen sulfide (H2S), like CO and NO, is an endogenous gas signal molecule that can play a vital role in many physiological processes. Therefore, it's of great significance to study the efficient and sensitive imaging technology of H2S in vivo. Copper is an essential trace element in the human body that is very important for many cellular functions and related metabolic processes. In this study we report the design of a peptide L (FITC-Ahx-His-Glu-Phe-His-NH2) that can act as an “On-Off-On” fluorescent chemical sensor to detect Cu2+ and S2−, and cysteine (Cys) and glutathione (GSH) can also replace Cu2+ from L-Cu system. The capability of this peptide fluorescent probe to detect H2S in HeLa cells is also evaluated.",

keywords = "Cells imaging, Copper ion detection, Hydrogen sulfide detection, Peptide-based",

author = "Chaowei Hao and Yongxin Li and Baomin Fan and Guangzhi Zeng and Danni Zhang and Zhaoxiang Bian and Jiang Wu",

note = "Funding Information: We are grateful to the National Natural Science Foundation of China ( 21572198 ) and the Applied Basic Research Project of Yunnan Province ( 2017FA004 ) and Yunnan Provincial Key Laboratory Construction Plan Funding and Yunnan Provincial Engineering Research Center Construction Plan Funding of Universities for their financial support. Funding Information: We are grateful to the National Natural Science Foundation of China (21572198) and the Applied Basic Research Project of Yunnan Province (2017FA004) and Yunnan Provincial Key LaboratoryConstruction Plan Funding and Yunnan Provincial Engineering Research Center Construction Plan Funding of Universities for their financial support.",

year = "2020",

month = may,

doi = "10.1016/j.microc.2020.104658",

language = "English",

volume = "154",

journal = "Microchemical Journal",

issn = "0026-265X",

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T1 - A new peptide-based chemosensor for selective imaging of copper ion and hydrogen sulfide in living cells

AU - Hao, Chaowei

AU - Li, Yongxin

AU - Fan, Baomin

AU - Zeng, Guangzhi

AU - Zhang, Danni

AU - Bian, Zhaoxiang

AU - Wu, Jiang

N1 - Funding Information: We are grateful to the National Natural Science Foundation of China ( 21572198 ) and the Applied Basic Research Project of Yunnan Province ( 2017FA004 ) and Yunnan Provincial Key Laboratory Construction Plan Funding and Yunnan Provincial Engineering Research Center Construction Plan Funding of Universities for their financial support. Funding Information: We are grateful to the National Natural Science Foundation of China (21572198) and the Applied Basic Research Project of Yunnan Province (2017FA004) and Yunnan Provincial Key LaboratoryConstruction Plan Funding and Yunnan Provincial Engineering Research Center Construction Plan Funding of Universities for their financial support.

PY - 2020/5

Y1 - 2020/5

N2 - Hydrogen sulfide (H2S), like CO and NO, is an endogenous gas signal molecule that can play a vital role in many physiological processes. Therefore, it's of great significance to study the efficient and sensitive imaging technology of H2S in vivo. Copper is an essential trace element in the human body that is very important for many cellular functions and related metabolic processes. In this study we report the design of a peptide L (FITC-Ahx-His-Glu-Phe-His-NH2) that can act as an “On-Off-On” fluorescent chemical sensor to detect Cu2+ and S2−, and cysteine (Cys) and glutathione (GSH) can also replace Cu2+ from L-Cu system. The capability of this peptide fluorescent probe to detect H2S in HeLa cells is also evaluated.

AB - Hydrogen sulfide (H2S), like CO and NO, is an endogenous gas signal molecule that can play a vital role in many physiological processes. Therefore, it's of great significance to study the efficient and sensitive imaging technology of H2S in vivo. Copper is an essential trace element in the human body that is very important for many cellular functions and related metabolic processes. In this study we report the design of a peptide L (FITC-Ahx-His-Glu-Phe-His-NH2) that can act as an “On-Off-On” fluorescent chemical sensor to detect Cu2+ and S2−, and cysteine (Cys) and glutathione (GSH) can also replace Cu2+ from L-Cu system. The capability of this peptide fluorescent probe to detect H2S in HeLa cells is also evaluated.

KW - Cells imaging

KW - Copper ion detection

KW - Hydrogen sulfide detection

KW - Peptide-based

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DO - 10.1016/j.microc.2020.104658

M3 - Journal article

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SN - 0026-265X

VL - 154

JO - Microchemical Journal

JF - Microchemical Journal

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A new peptide-based chemosensor for selective imaging of copper ion and hydrogen sulfide in living cells

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