A new method for the analysis of β2-agonists in human urine by pressure-assisted capillary electrochromatography coupled with electrospray ionization-mass spectrometry using a silica-based monolithic column

Minghua Lu, Lan Zhang, Xin Li, Qiaomei Lu, Guonan Chen, Zongwei CAI*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

A new pressure-assisted capillary electrochromatography coupled with electrospray ionization-mass spectrometry method using a silica-based monolithic column as separation media was developed for the analysis of β2-agonists in human urine. Experimental conditions including the mobile phase, separation voltage, assisted pressure, and sheath liquid were optimized for the analysis: mobile phase composed of 82% (v/v) ACN and 18% (v/v) 20 mmol/L ammonium acetate (pH 6.0); separation voltage 25 kV; assisted pressure 2 bar; and the sheath liquid consisting of 7.5 mmol/L acetic acid in isopropanol/water 50/50% (v/v) that was delivered at a flow rate of 3.0 μL/min. Six β2-agonists were separated within 12.5 min with LODs (defined as S/N = 3) in the range of 0.25-2.0 ng/mL. The absolute LODs of the developed method for analyzing six β2-agonists ranged from 5.75 to 46.0 fg. Method repeatability of run-to-run and column-to-column was satisfactory. The recovery obtained from the analysis of spiked urine samples was between 88.2% and 106% with RSDs lower than 6.68%. The method was successfully applied to the analysis of real urine sample from volunteers.

Original languageEnglish
Pages (from-to)1655-1661
Number of pages7
JournalTalanta
Volume81
Issue number4-5
DOIs
Publication statusPublished - 15 Jun 2010

Scopus Subject Areas

  • Analytical Chemistry

User-Defined Keywords

  • Capillary electrochromatography
  • Electrospray ionization-mass spectrometry
  • Silica-based monolithic column

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