A new method for identification of in vitro metabolites of 2,3,7,8-TCDD with rat liver microsomes by using liquid chromatography-mass spectrometry

Chunyan Wang, Zhi Tang, Yanling Zhang, Yan Liang, Fengrui Song, Zongwei CAI*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

2,3,7,8-Tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) is known as a highly toxic environmental contaminant and poses potential risks to human and animal health. As a persistent organic pollutant, 2,3,7,8-TCDD is hard to metabolize and thus gets accumulated in the human and animal body. Literature reports on the metabolism study of 2,3,7,8-TCDD are rare. The traditional method of GC-MS for 2,3,7,8-TCDD analysis might not be amenable for the direct analysis of its polar metabolites. In this study, in vitro metabolism of 2,3,7,8-TCDD with rat liver microsomes by using LC-MS and MS/MS was investigated and two hydroxylated metabolites of 2,3,7,8-TCDD and four trichloro-dihydroxydibenzo-p-dioxins were identified from the direct LC-MS and MS/MS analyses of the incubated samples.

Original languageEnglish
Pages (from-to)2757-2760
Number of pages4
JournalAnalytical Methods
Volume5
Issue number11
DOIs
Publication statusPublished - 7 Jun 2013

Scopus Subject Areas

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Engineering(all)

Fingerprint

Dive into the research topics of 'A new method for identification of in vitro metabolites of 2,3,7,8-TCDD with rat liver microsomes by using liquid chromatography-mass spectrometry'. Together they form a unique fingerprint.

Cite this