A new method for identification of in vitro metabolites of 2,3,7,8-TCDD with rat liver microsomes by using liquid chromatography-mass spectrometry

Chunyan Wang; Zhi Tang; Yanling Zhang; Yan Liang; Fengrui Song; Zongwei CAI

doi:10.1039/c3ay26443b

A new method for identification of in vitro metabolites of 2,3,7,8-TCDD with rat liver microsomes by using liquid chromatography-mass spectrometry

Chunyan Wang, Zhi Tang, Yanling Zhang, Yan Liang, Fengrui Song, Zongwei CAI^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

5 Citations (Scopus)

Abstract

2,3,7,8-Tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) is known as a highly toxic environmental contaminant and poses potential risks to human and animal health. As a persistent organic pollutant, 2,3,7,8-TCDD is hard to metabolize and thus gets accumulated in the human and animal body. Literature reports on the metabolism study of 2,3,7,8-TCDD are rare. The traditional method of GC-MS for 2,3,7,8-TCDD analysis might not be amenable for the direct analysis of its polar metabolites. In this study, in vitro metabolism of 2,3,7,8-TCDD with rat liver microsomes by using LC-MS and MS/MS was investigated and two hydroxylated metabolites of 2,3,7,8-TCDD and four trichloro-dihydroxydibenzo-p-dioxins were identified from the direct LC-MS and MS/MS analyses of the incubated samples.

Original language	English
Pages (from-to)	2757-2760
Number of pages	4
Journal	Analytical Methods
Volume	5
Issue number	11
DOIs	https://doi.org/10.1039/c3ay26443b
Publication status	Published - 7 Jun 2013

Scopus Subject Areas

Analytical Chemistry
General Chemical Engineering
General Engineering

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@article{5177523d337044b68fd304e585516b16,

title = "A new method for identification of in vitro metabolites of 2,3,7,8-TCDD with rat liver microsomes by using liquid chromatography-mass spectrometry",

abstract = "2,3,7,8-Tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) is known as a highly toxic environmental contaminant and poses potential risks to human and animal health. As a persistent organic pollutant, 2,3,7,8-TCDD is hard to metabolize and thus gets accumulated in the human and animal body. Literature reports on the metabolism study of 2,3,7,8-TCDD are rare. The traditional method of GC-MS for 2,3,7,8-TCDD analysis might not be amenable for the direct analysis of its polar metabolites. In this study, in vitro metabolism of 2,3,7,8-TCDD with rat liver microsomes by using LC-MS and MS/MS was investigated and two hydroxylated metabolites of 2,3,7,8-TCDD and four trichloro-dihydroxydibenzo-p-dioxins were identified from the direct LC-MS and MS/MS analyses of the incubated samples.",

author = "Chunyan Wang and Zhi Tang and Yanling Zhang and Yan Liang and Fengrui Song and Zongwei CAI",

note = "The authors would like to acknowledge the financial support of a Faculty Research Grant from Hong Kong Baptist University (FRG2/10-11/029) and General Research Grant from the Research Grant Committee of Hong Kong SAR (HKBU200310).",

year = "2013",

month = jun,

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doi = "10.1039/c3ay26443b",

language = "English",

volume = "5",

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journal = "Analytical Methods",

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TY - JOUR

T1 - A new method for identification of in vitro metabolites of 2,3,7,8-TCDD with rat liver microsomes by using liquid chromatography-mass spectrometry

AU - Wang, Chunyan

AU - Tang, Zhi

AU - Zhang, Yanling

AU - Liang, Yan

AU - Song, Fengrui

AU - CAI, Zongwei

N1 - The authors would like to acknowledge the financial support of a Faculty Research Grant from Hong Kong Baptist University (FRG2/10-11/029) and General Research Grant from the Research Grant Committee of Hong Kong SAR (HKBU200310).

PY - 2013/6/7

Y1 - 2013/6/7

N2 - 2,3,7,8-Tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) is known as a highly toxic environmental contaminant and poses potential risks to human and animal health. As a persistent organic pollutant, 2,3,7,8-TCDD is hard to metabolize and thus gets accumulated in the human and animal body. Literature reports on the metabolism study of 2,3,7,8-TCDD are rare. The traditional method of GC-MS for 2,3,7,8-TCDD analysis might not be amenable for the direct analysis of its polar metabolites. In this study, in vitro metabolism of 2,3,7,8-TCDD with rat liver microsomes by using LC-MS and MS/MS was investigated and two hydroxylated metabolites of 2,3,7,8-TCDD and four trichloro-dihydroxydibenzo-p-dioxins were identified from the direct LC-MS and MS/MS analyses of the incubated samples.

AB - 2,3,7,8-Tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) is known as a highly toxic environmental contaminant and poses potential risks to human and animal health. As a persistent organic pollutant, 2,3,7,8-TCDD is hard to metabolize and thus gets accumulated in the human and animal body. Literature reports on the metabolism study of 2,3,7,8-TCDD are rare. The traditional method of GC-MS for 2,3,7,8-TCDD analysis might not be amenable for the direct analysis of its polar metabolites. In this study, in vitro metabolism of 2,3,7,8-TCDD with rat liver microsomes by using LC-MS and MS/MS was investigated and two hydroxylated metabolites of 2,3,7,8-TCDD and four trichloro-dihydroxydibenzo-p-dioxins were identified from the direct LC-MS and MS/MS analyses of the incubated samples.

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U2 - 10.1039/c3ay26443b

DO - 10.1039/c3ay26443b

M3 - Journal article

AN - SCOPUS:84878042970

SN - 1759-9660

VL - 5

SP - 2757

EP - 2760

JO - Analytical Methods

JF - Analytical Methods

IS - 11

ER -

A new method for identification of in vitro metabolites of 2,3,7,8-TCDD with rat liver microsomes by using liquid chromatography-mass spectrometry

Abstract

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UN SDGs

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