A new approach for the sensitive determination of DNA adduct of aristolochic acid II by using high-performance liquid chromatography with fluorescence detection

Wan Chan; Wing Tat Poon; Yan Wo Chan; King Yi Wan; Zongwei CAI

doi:10.1016/j.jchromb.2009.02.007

A new approach for the sensitive determination of DNA adduct of aristolochic acid II by using high-performance liquid chromatography with fluorescence detection

Wan Chan, Wing Tat Poon, Yan Wo Chan, King Yi Wan, Zongwei CAI^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

7 Citations (Scopus)

Abstract

A sensitive high-performance liquid chromatography method with fluorescence detection (HPLC-FLD) for the determination of DNA adducts induced by nephrotoxic and carcinogenic aristolochic acid (AA) is presented. The DNA adduct of AAII (dA-AAII) was synthesized by in vitro incubation, purified by preparative HPLC, characterized using fluorescence spectroscopy and high-resolution mass spectrometry, and was used as the biomarker for AA exposure in rats. The developed HPLC-FLD method was validated and applied for the determination of dA-AAII in rat kidney tissues. The method provided a detection limit of 18.3 fmol, which allowed the detection of dA-AAII in the rat kidney tissue samples collected after a single oral dose of AA. dA-AAII was detected in the kidney DNA digestion extracts of the rats that were dosed with AA at 5 mg/kg and 30 mg/kg at concentrations of 6.2 ± 1.1 and 41.3 ± 8.0 dA-AAII per 10⁹ normal dA, respectively.

Original language	English
Pages (from-to)	848-852
Number of pages	5
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	877
Issue number	10
DOIs	https://doi.org/10.1016/j.jchromb.2009.02.007
Publication status	Published - 1 Apr 2009

User-Defined Keywords

Aristolochic acid
DNA-AA adduct
Fluorescence detection
High-performance liquid chromatography
Kidney tissue

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10.1016/j.jchromb.2009.02.007

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Chan, W., Poon, W. T., Chan, Y. W., Wan, K. Y., & CAI, Z. (2009). A new approach for the sensitive determination of DNA adduct of aristolochic acid II by using high-performance liquid chromatography with fluorescence detection. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 877(10), 848-852. https://doi.org/10.1016/j.jchromb.2009.02.007

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title = "A new approach for the sensitive determination of DNA adduct of aristolochic acid II by using high-performance liquid chromatography with fluorescence detection",

abstract = "A sensitive high-performance liquid chromatography method with fluorescence detection (HPLC-FLD) for the determination of DNA adducts induced by nephrotoxic and carcinogenic aristolochic acid (AA) is presented. The DNA adduct of AAII (dA-AAII) was synthesized by in vitro incubation, purified by preparative HPLC, characterized using fluorescence spectroscopy and high-resolution mass spectrometry, and was used as the biomarker for AA exposure in rats. The developed HPLC-FLD method was validated and applied for the determination of dA-AAII in rat kidney tissues. The method provided a detection limit of 18.3 fmol, which allowed the detection of dA-AAII in the rat kidney tissue samples collected after a single oral dose of AA. dA-AAII was detected in the kidney DNA digestion extracts of the rats that were dosed with AA at 5 mg/kg and 30 mg/kg at concentrations of 6.2 ± 1.1 and 41.3 ± 8.0 dA-AAII per 109 normal dA, respectively.",

keywords = "Aristolochic acid, DNA-AA adduct, Fluorescence detection, High-performance liquid chromatography, Kidney tissue",

author = "Wan Chan and Poon, {Wing Tat} and Chan, {Yan Wo} and Wan, {King Yi} and Zongwei CAI",

note = "Funding Information: The financial supports of the Research Grant Council, University Grants Committee of Hong Kong (HKBU2459/06M), the Health and Health Services Research Fund from Food and Health Bureau of Hong Kong (05060141), and the Faculty Research Grant of Hong Kong Baptist University (FRG/06-07/II-56) on this study are acknowledged.",

year = "2009",

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doi = "10.1016/j.jchromb.2009.02.007",

language = "English",

volume = "877",

pages = "848--852",

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Chan, W, Poon, WT, Chan, YW, Wan, KY & CAI, Z 2009, 'A new approach for the sensitive determination of DNA adduct of aristolochic acid II by using high-performance liquid chromatography with fluorescence detection', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 877, no. 10, pp. 848-852. https://doi.org/10.1016/j.jchromb.2009.02.007

A new approach for the sensitive determination of DNA adduct of aristolochic acid II by using high-performance liquid chromatography with fluorescence detection. / Chan, Wan; Poon, Wing Tat; Chan, Yan Wo et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 877, No. 10, 01.04.2009, p. 848-852.

Research output: Contribution to journal › Journal article › peer-review

TY - JOUR

T1 - A new approach for the sensitive determination of DNA adduct of aristolochic acid II by using high-performance liquid chromatography with fluorescence detection

AU - Chan, Wan

AU - Poon, Wing Tat

AU - Chan, Yan Wo

AU - Wan, King Yi

AU - CAI, Zongwei

N1 - Funding Information: The financial supports of the Research Grant Council, University Grants Committee of Hong Kong (HKBU2459/06M), the Health and Health Services Research Fund from Food and Health Bureau of Hong Kong (05060141), and the Faculty Research Grant of Hong Kong Baptist University (FRG/06-07/II-56) on this study are acknowledged.

PY - 2009/4/1

Y1 - 2009/4/1

N2 - A sensitive high-performance liquid chromatography method with fluorescence detection (HPLC-FLD) for the determination of DNA adducts induced by nephrotoxic and carcinogenic aristolochic acid (AA) is presented. The DNA adduct of AAII (dA-AAII) was synthesized by in vitro incubation, purified by preparative HPLC, characterized using fluorescence spectroscopy and high-resolution mass spectrometry, and was used as the biomarker for AA exposure in rats. The developed HPLC-FLD method was validated and applied for the determination of dA-AAII in rat kidney tissues. The method provided a detection limit of 18.3 fmol, which allowed the detection of dA-AAII in the rat kidney tissue samples collected after a single oral dose of AA. dA-AAII was detected in the kidney DNA digestion extracts of the rats that were dosed with AA at 5 mg/kg and 30 mg/kg at concentrations of 6.2 ± 1.1 and 41.3 ± 8.0 dA-AAII per 109 normal dA, respectively.

AB - A sensitive high-performance liquid chromatography method with fluorescence detection (HPLC-FLD) for the determination of DNA adducts induced by nephrotoxic and carcinogenic aristolochic acid (AA) is presented. The DNA adduct of AAII (dA-AAII) was synthesized by in vitro incubation, purified by preparative HPLC, characterized using fluorescence spectroscopy and high-resolution mass spectrometry, and was used as the biomarker for AA exposure in rats. The developed HPLC-FLD method was validated and applied for the determination of dA-AAII in rat kidney tissues. The method provided a detection limit of 18.3 fmol, which allowed the detection of dA-AAII in the rat kidney tissue samples collected after a single oral dose of AA. dA-AAII was detected in the kidney DNA digestion extracts of the rats that were dosed with AA at 5 mg/kg and 30 mg/kg at concentrations of 6.2 ± 1.1 and 41.3 ± 8.0 dA-AAII per 109 normal dA, respectively.

KW - Aristolochic acid

KW - DNA-AA adduct

KW - Fluorescence detection

KW - High-performance liquid chromatography

KW - Kidney tissue

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U2 - 10.1016/j.jchromb.2009.02.007

DO - 10.1016/j.jchromb.2009.02.007

M3 - Journal article

C2 - 19249256

AN - SCOPUS:62249197501

SN - 1570-0232

VL - 877

SP - 848

EP - 852

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 10

ER -

A new approach for the sensitive determination of DNA adduct of aristolochic acid II by using high-performance liquid chromatography with fluorescence detection

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