A new approach for simultaneous screening and quantification of toxic pyrrolizidine alkaloids in some potential pyrrolizidine alkaloid-containing plants by using ultra performance liquid chromatography-tandem quadrupole mass spectrometry

Yan Zhou; Na Li; Franky Fung Kei Choi; Chun Feng Qiao; Jing Zheng Song; Song Lin Li; Xin Liu; Zongwei CAI; Peter P. Fu; Ge Lin; Hong Xi Xu

doi:10.1016/j.aca.2010.09.011

A new approach for simultaneous screening and quantification of toxic pyrrolizidine alkaloids in some potential pyrrolizidine alkaloid-containing plants by using ultra performance liquid chromatography-tandem quadrupole mass spectrometry

Yan Zhou, Na Li, Franky Fung Kei Choi, Chun Feng Qiao, Jing Zheng Song, Song Lin Li, Xin Liu, Zongwei CAI, Peter P. Fu, Ge Lin, Hong Xi Xu

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

64 Citations (Scopus)

Abstract

A rapid, but sensitive and selective method for simultaneous screening and quantification of toxic pyrrolizidine alkaloids (PAs) by ultra performance liquid-chromatography (UPLC) coupled with tandem mass spectrometry (MS/MS) on a tandem quadrupole mass spectrometer (TQ-MS) is described. This was accomplished by incorporating the precursor ion scan (PIS) acquisition and multiple reaction monitoring (MRM) acquisition in the same UPLC-MS/MS run. Notably, the developed PIS approach for detecting two pairs of characteristic product ions at m/. z 120/138 or 168/150, allowed specific identification of toxic retronecine and otonecine types PAs. This PIS method is highly sensitive with over 10-fold sensitivity improvement upon previously published LC-MS method. Moreover, this new approach is suitable for high sample throughput and was applied to the screening and quantifying toxic PAs in 22 samples collected from seven. Parasenecio species and four Senecio species. In addition, coupling the MRM with PIS approach generated quantitative results equivalent to those obtained by conventional MRM-only approach. This coupled MRM with PIS approach could provide both qualitative and quantitative results without the need of repetitive analyses.

Original language	English
Pages (from-to)	33-40
Number of pages	8
Journal	Analytica Chimica Acta
Volume	681
Issue number	1-2
DOIs	https://doi.org/10.1016/j.aca.2010.09.011
Publication status	Published - 29 Nov 2010

Scopus Subject Areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy

User-Defined Keywords

Multiple reaction monitoring (MRM)
Parasenecio species
Precursor ion scan (PIS)
Pyrrolizidine alkaloids (PAs)
Senecio species
UPLC-TQ-MS

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10.1016/j.aca.2010.09.011

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Zhou, Y., Li, N., Choi, F. F. K., Qiao, C. F., Song, J. Z., Li, S. L., Liu, X., CAI, Z., Fu, P. P., Lin, G., & Xu, H. X. (2010). A new approach for simultaneous screening and quantification of toxic pyrrolizidine alkaloids in some potential pyrrolizidine alkaloid-containing plants by using ultra performance liquid chromatography-tandem quadrupole mass spectrometry. Analytica Chimica Acta, 681(1-2), 33-40. https://doi.org/10.1016/j.aca.2010.09.011

@article{d479e6e9f2b04aa8a61f690c5ea8004d,

title = "A new approach for simultaneous screening and quantification of toxic pyrrolizidine alkaloids in some potential pyrrolizidine alkaloid-containing plants by using ultra performance liquid chromatography-tandem quadrupole mass spectrometry",

abstract = "A rapid, but sensitive and selective method for simultaneous screening and quantification of toxic pyrrolizidine alkaloids (PAs) by ultra performance liquid-chromatography (UPLC) coupled with tandem mass spectrometry (MS/MS) on a tandem quadrupole mass spectrometer (TQ-MS) is described. This was accomplished by incorporating the precursor ion scan (PIS) acquisition and multiple reaction monitoring (MRM) acquisition in the same UPLC-MS/MS run. Notably, the developed PIS approach for detecting two pairs of characteristic product ions at m/. z 120/138 or 168/150, allowed specific identification of toxic retronecine and otonecine types PAs. This PIS method is highly sensitive with over 10-fold sensitivity improvement upon previously published LC-MS method. Moreover, this new approach is suitable for high sample throughput and was applied to the screening and quantifying toxic PAs in 22 samples collected from seven. Parasenecio species and four Senecio species. In addition, coupling the MRM with PIS approach generated quantitative results equivalent to those obtained by conventional MRM-only approach. This coupled MRM with PIS approach could provide both qualitative and quantitative results without the need of repetitive analyses.",

keywords = "Multiple reaction monitoring (MRM), Parasenecio species, Precursor ion scan (PIS), Pyrrolizidine alkaloids (PAs), Senecio species, UPLC-TQ-MS",

author = "Yan Zhou and Na Li and Choi, {Franky Fung Kei} and Qiao, {Chun Feng} and Song, {Jing Zheng} and Li, {Song Lin} and Xin Liu and Zongwei CAI and Fu, {Peter P.} and Ge Lin and Xu, {Hong Xi}",

note = "Funding Information: This research was supported by the Hong Kong Jockey Club Charities Trust (JCICM-15-07) . The authors are grateful to Dr. Karl Lo of Waters Corp. The contents of this article do not necessarily reflect the views and policies of the U.S. Food & Drug Administration. The mentioning of trade names and commercial products does not constitute endorsement or recommendation for use. ",

year = "2010",

month = nov,

day = "29",

doi = "10.1016/j.aca.2010.09.011",

language = "English",

volume = "681",

pages = "33--40",

journal = "Analytica Chimica Acta",

issn = "0003-2670",

publisher = "Elsevier",

number = "1-2",

}

Zhou, Y, Li, N, Choi, FFK, Qiao, CF, Song, JZ, Li, SL, Liu, X, CAI, Z, Fu, PP, Lin, G & Xu, HX 2010, 'A new approach for simultaneous screening and quantification of toxic pyrrolizidine alkaloids in some potential pyrrolizidine alkaloid-containing plants by using ultra performance liquid chromatography-tandem quadrupole mass spectrometry', Analytica Chimica Acta, vol. 681, no. 1-2, pp. 33-40. https://doi.org/10.1016/j.aca.2010.09.011

A new approach for simultaneous screening and quantification of toxic pyrrolizidine alkaloids in some potential pyrrolizidine alkaloid-containing plants by using ultra performance liquid chromatography-tandem quadrupole mass spectrometry. / Zhou, Yan; Li, Na; Choi, Franky Fung Kei et al.
In: Analytica Chimica Acta, Vol. 681, No. 1-2, 29.11.2010, p. 33-40.

Research output: Contribution to journal › Journal article › peer-review

TY - JOUR

T1 - A new approach for simultaneous screening and quantification of toxic pyrrolizidine alkaloids in some potential pyrrolizidine alkaloid-containing plants by using ultra performance liquid chromatography-tandem quadrupole mass spectrometry

AU - Zhou, Yan

AU - Li, Na

AU - Choi, Franky Fung Kei

AU - Qiao, Chun Feng

AU - Song, Jing Zheng

AU - Li, Song Lin

AU - Liu, Xin

AU - CAI, Zongwei

AU - Fu, Peter P.

AU - Lin, Ge

AU - Xu, Hong Xi

N1 - Funding Information: This research was supported by the Hong Kong Jockey Club Charities Trust (JCICM-15-07) . The authors are grateful to Dr. Karl Lo of Waters Corp. The contents of this article do not necessarily reflect the views and policies of the U.S. Food & Drug Administration. The mentioning of trade names and commercial products does not constitute endorsement or recommendation for use.

PY - 2010/11/29

Y1 - 2010/11/29

N2 - A rapid, but sensitive and selective method for simultaneous screening and quantification of toxic pyrrolizidine alkaloids (PAs) by ultra performance liquid-chromatography (UPLC) coupled with tandem mass spectrometry (MS/MS) on a tandem quadrupole mass spectrometer (TQ-MS) is described. This was accomplished by incorporating the precursor ion scan (PIS) acquisition and multiple reaction monitoring (MRM) acquisition in the same UPLC-MS/MS run. Notably, the developed PIS approach for detecting two pairs of characteristic product ions at m/. z 120/138 or 168/150, allowed specific identification of toxic retronecine and otonecine types PAs. This PIS method is highly sensitive with over 10-fold sensitivity improvement upon previously published LC-MS method. Moreover, this new approach is suitable for high sample throughput and was applied to the screening and quantifying toxic PAs in 22 samples collected from seven. Parasenecio species and four Senecio species. In addition, coupling the MRM with PIS approach generated quantitative results equivalent to those obtained by conventional MRM-only approach. This coupled MRM with PIS approach could provide both qualitative and quantitative results without the need of repetitive analyses.

AB - A rapid, but sensitive and selective method for simultaneous screening and quantification of toxic pyrrolizidine alkaloids (PAs) by ultra performance liquid-chromatography (UPLC) coupled with tandem mass spectrometry (MS/MS) on a tandem quadrupole mass spectrometer (TQ-MS) is described. This was accomplished by incorporating the precursor ion scan (PIS) acquisition and multiple reaction monitoring (MRM) acquisition in the same UPLC-MS/MS run. Notably, the developed PIS approach for detecting two pairs of characteristic product ions at m/. z 120/138 or 168/150, allowed specific identification of toxic retronecine and otonecine types PAs. This PIS method is highly sensitive with over 10-fold sensitivity improvement upon previously published LC-MS method. Moreover, this new approach is suitable for high sample throughput and was applied to the screening and quantifying toxic PAs in 22 samples collected from seven. Parasenecio species and four Senecio species. In addition, coupling the MRM with PIS approach generated quantitative results equivalent to those obtained by conventional MRM-only approach. This coupled MRM with PIS approach could provide both qualitative and quantitative results without the need of repetitive analyses.

KW - Multiple reaction monitoring (MRM)

KW - Parasenecio species

KW - Precursor ion scan (PIS)

KW - Pyrrolizidine alkaloids (PAs)

KW - Senecio species

KW - UPLC-TQ-MS

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U2 - 10.1016/j.aca.2010.09.011

DO - 10.1016/j.aca.2010.09.011

M3 - Journal article

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AN - SCOPUS:78049280983

SN - 0003-2670

VL - 681

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JO - Analytica Chimica Acta

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ER -

Zhou Y, Li N, Choi FFK, Qiao CF, Song JZ, Li SL et al. A new approach for simultaneous screening and quantification of toxic pyrrolizidine alkaloids in some potential pyrrolizidine alkaloid-containing plants by using ultra performance liquid chromatography-tandem quadrupole mass spectrometry. Analytica Chimica Acta. 2010 Nov 29;681(1-2):33-40. doi: 10.1016/j.aca.2010.09.011

A new approach for simultaneous screening and quantification of toxic pyrrolizidine alkaloids in some potential pyrrolizidine alkaloid-containing plants by using ultra performance liquid chromatography-tandem quadrupole mass spectrometry

Abstract

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