A new approach for simultaneous screening and quantification of toxic pyrrolizidine alkaloids in some potential pyrrolizidine alkaloid-containing plants by using ultra performance liquid chromatography-tandem quadrupole mass spectrometry

Yan Zhou, Na Li, Franky Fung Kei Choi, Chun Feng Qiao, Jing Zheng Song, Song Lin Li, Xin Liu, Zongwei CAI, Peter P. Fu, Ge Lin, Hong Xi Xu

Research output: Contribution to journalArticlepeer-review

50 Citations (Scopus)

Abstract

A rapid, but sensitive and selective method for simultaneous screening and quantification of toxic pyrrolizidine alkaloids (PAs) by ultra performance liquid-chromatography (UPLC) coupled with tandem mass spectrometry (MS/MS) on a tandem quadrupole mass spectrometer (TQ-MS) is described. This was accomplished by incorporating the precursor ion scan (PIS) acquisition and multiple reaction monitoring (MRM) acquisition in the same UPLC-MS/MS run. Notably, the developed PIS approach for detecting two pairs of characteristic product ions at m/. z 120/138 or 168/150, allowed specific identification of toxic retronecine and otonecine types PAs. This PIS method is highly sensitive with over 10-fold sensitivity improvement upon previously published LC-MS method. Moreover, this new approach is suitable for high sample throughput and was applied to the screening and quantifying toxic PAs in 22 samples collected from seven. Parasenecio species and four Senecio species. In addition, coupling the MRM with PIS approach generated quantitative results equivalent to those obtained by conventional MRM-only approach. This coupled MRM with PIS approach could provide both qualitative and quantitative results without the need of repetitive analyses.

Original languageEnglish
Pages (from-to)33-40
Number of pages8
JournalAnalytica Chimica Acta
Volume681
Issue number1-2
DOIs
Publication statusPublished - 29 Nov 2010

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

User-Defined Keywords

  • Multiple reaction monitoring (MRM)
  • Parasenecio species
  • Precursor ion scan (PIS)
  • Pyrrolizidine alkaloids (PAs)
  • Senecio species
  • UPLC-TQ-MS

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