TY - JOUR
T1 - A new application of an aqueous diphase solvent system in one-step preparation of polysaccharide from the crude water extract of Radix Astragali by high-speed counter-current chromatography
AU - Yin, Jun Yi
AU - JIANG, Zhi Hong
AU - Yu, Hua
AU - Xie, Ming Yong
AU - HSIAO, Wendy W L
AU - LYU, Aiping
AU - HAN, Simon Q B
N1 - Funding Information:
This research is funded by the Innovation and Technology Fund (ITS/311/09 and InP/108/10) of the Government of the Hong Kong Special Administrative Region and Hong Kong Baptist University ( FRG2/11-12/048 ).
PY - 2012/11/2
Y1 - 2012/11/2
N2 - Polysaccharide's purification remains challenge to separation technology. Conventional methods involve time-consuming and complicated operations and always cause significant variation in the isolates' chemistry. This paper reports an aqueous diphase solvent system, namely PEG1000-MgSO4-H2O, which succeeded in one-step CCC separation of a polysaccharide (43mg) from the water extract (1.67g) of Radix Astragali. The solvent composition was set as 12:16:72 (w/w/w) of which the lower phase was used as the mobile phase at a flow rate of 0.8mL/min in a 1000mL column. The purified polysaccharide bears an average molecular weight of 1095kDa and consists of galacturonic acid (76.5%), galactose (7.7%), arabinose (4.2%) and glucose (5.0%). Methylation analysis result showed it was composed of 58.4% of 1,4-linked Glcp, 11.8% of T-linked Araf, 10.5% of T-linked Glcp, 9.1% of 1,4,6-linked Galp and 5.1% of 1,3,6-linked Galp, etc. This success shows a short way between the crude water extract and purified polysaccharides, which minimizes the chemical variation caused by purification methods.
AB - Polysaccharide's purification remains challenge to separation technology. Conventional methods involve time-consuming and complicated operations and always cause significant variation in the isolates' chemistry. This paper reports an aqueous diphase solvent system, namely PEG1000-MgSO4-H2O, which succeeded in one-step CCC separation of a polysaccharide (43mg) from the water extract (1.67g) of Radix Astragali. The solvent composition was set as 12:16:72 (w/w/w) of which the lower phase was used as the mobile phase at a flow rate of 0.8mL/min in a 1000mL column. The purified polysaccharide bears an average molecular weight of 1095kDa and consists of galacturonic acid (76.5%), galactose (7.7%), arabinose (4.2%) and glucose (5.0%). Methylation analysis result showed it was composed of 58.4% of 1,4-linked Glcp, 11.8% of T-linked Araf, 10.5% of T-linked Glcp, 9.1% of 1,4,6-linked Galp and 5.1% of 1,3,6-linked Galp, etc. This success shows a short way between the crude water extract and purified polysaccharides, which minimizes the chemical variation caused by purification methods.
KW - Aqueous diphase solvent system
KW - High-speed counter-current chromatography
KW - Polysaccharide
KW - Radix Astragali
UR - http://www.scopus.com/inward/record.url?scp=84867221029&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2012.08.099
DO - 10.1016/j.chroma.2012.08.099
M3 - Journal article
C2 - 22999203
AN - SCOPUS:84867221029
SN - 0021-9673
VL - 1262
SP - 92
EP - 97
JO - Journal of Chromatography A
JF - Journal of Chromatography A
ER -