A new application of an aqueous diphase solvent system in one-step preparation of polysaccharide from the crude water extract of Radix Astragali by high-speed counter-current chromatography

Jun Yi Yin, Zhi Hong JIANG, Hua Yu, Ming Yong Xie*, Wendy W L HSIAO, Aiping LYU, Simon Q B HAN

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Polysaccharide's purification remains challenge to separation technology. Conventional methods involve time-consuming and complicated operations and always cause significant variation in the isolates' chemistry. This paper reports an aqueous diphase solvent system, namely PEG1000-MgSO4-H2O, which succeeded in one-step CCC separation of a polysaccharide (43mg) from the water extract (1.67g) of Radix Astragali. The solvent composition was set as 12:16:72 (w/w/w) of which the lower phase was used as the mobile phase at a flow rate of 0.8mL/min in a 1000mL column. The purified polysaccharide bears an average molecular weight of 1095kDa and consists of galacturonic acid (76.5%), galactose (7.7%), arabinose (4.2%) and glucose (5.0%). Methylation analysis result showed it was composed of 58.4% of 1,4-linked Glcp, 11.8% of T-linked Araf, 10.5% of T-linked Glcp, 9.1% of 1,4,6-linked Galp and 5.1% of 1,3,6-linked Galp, etc. This success shows a short way between the crude water extract and purified polysaccharides, which minimizes the chemical variation caused by purification methods.

Original languageEnglish
Pages (from-to)92-97
Number of pages6
JournalJournal of Chromatography A
Volume1262
DOIs
Publication statusPublished - 2 Nov 2012

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

User-Defined Keywords

  • Aqueous diphase solvent system
  • High-speed counter-current chromatography
  • Polysaccharide
  • Radix Astragali

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