A NASBA method to detect high- and low-pathogenicity H5 avian influenza viruses

R. A. Collins; L. S. Ko; K. L. So; T. Ellis; L. T. Lau; A. C. H Yu

doi:10.1637/0005-2086-47.s3.1069

A NASBA method to detect high- and low-pathogenicity H5 avian influenza viruses

R. A. Collins, L. S. Ko, K. L. So, T. Ellis, L. T. Lau, A. C. H Yu^*

^*Corresponding author for this work

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Research output: Contribution to journal › Journal article › peer-review

27 Citations (Scopus)

Abstract

Nucleic acid sequence-based amplification (NASBA) allows the rapid amplification of specific regions of nucleic acid obtained from a diverse range of sources. It is especially suitable for amplifying RNA sequences. A NASBA technique was developed that allows the detection of avian influenza A subtype H5 from allantoic fluid harvested from inoculated chick embryos. The amplified viral RNA is detected by electrochemiluminescence. The described NASBA technique is a specific, rapid, and sensitive method of detection of influenza A subtype H5 viruses. More importantly, it can be used to distinguish high- and low-pathogenicity strains of the H5 subtype.

Original language	English
Pages (from-to)	1069-1074
Number of pages	6
Journal	Avian Diseases
Volume	47
Issue number	S3
DOIs	https://doi.org/10.1637/0005-2086-47.s3.1069
Publication status	Published - 1 Sept 2003

User-Defined Keywords

Avian influenza
Electrochemiluminescence
H5
Hemagglutinin
NASBA
Pathogenic

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1637/0005-2086-47.s3.1069

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title = "A NASBA method to detect high- and low-pathogenicity H5 avian influenza viruses",

abstract = "Nucleic acid sequence-based amplification (NASBA) allows the rapid amplification of specific regions of nucleic acid obtained from a diverse range of sources. It is especially suitable for amplifying RNA sequences. A NASBA technique was developed that allows the detection of avian influenza A subtype H5 from allantoic fluid harvested from inoculated chick embryos. The amplified viral RNA is detected by electrochemiluminescence. The described NASBA technique is a specific, rapid, and sensitive method of detection of influenza A subtype H5 viruses. More importantly, it can be used to distinguish high- and low-pathogenicity strains of the H5 subtype.",

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AU - Collins, R. A.

AU - Ko, L. S.

AU - So, K. L.

AU - Ellis, T.

AU - Lau, L. T.

AU - Yu, A. C. H

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AB - Nucleic acid sequence-based amplification (NASBA) allows the rapid amplification of specific regions of nucleic acid obtained from a diverse range of sources. It is especially suitable for amplifying RNA sequences. A NASBA technique was developed that allows the detection of avian influenza A subtype H5 from allantoic fluid harvested from inoculated chick embryos. The amplified viral RNA is detected by electrochemiluminescence. The described NASBA technique is a specific, rapid, and sensitive method of detection of influenza A subtype H5 viruses. More importantly, it can be used to distinguish high- and low-pathogenicity strains of the H5 subtype.

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KW - Electrochemiluminescence

KW - H5

KW - Hemagglutinin

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KW - Pathogenic

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JO - Avian Diseases

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A NASBA method to detect high- and low-pathogenicity H5 avian influenza viruses

Abstract

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