TY - JOUR
T1 - A model to induce low temperature trauma for in vitro astrogliosis study
AU - Yu, Albert Cheung Hoi
AU - Wu, Bing Yi
AU - Liu, Rong Yu
AU - Li, Qiang
AU - Li, Yue Xin
AU - Wong, Pui Fan
AU - Liu, Shuang
AU - Lau, Lok Ting
AU - Fung, Yin Wan Wendy
N1 - This study was supported by a Grant from the Shanghai Research Center of Life Sciences, Chinese Academy of Sciences; a Research Grant Council (H.K.) award (HKUST6177/97M), HKUST/CAS Joint Laboratory Scheme award, a Hong Kong North American Medical Association Foundation Grant (NAMA 94/95.SC01), the National Natural Science Foundation of China (30270426) and the Beijing Natural Science Foundation (7032026) to ACHY.
Publisher Copyright:
© 2004 Springer Science+Business Media, Inc.
PY - 2004/11
Y1 - 2004/11
N2 - Astrogliosis is an inevitable and rapid response of astrocytes to physical, chemical and pathological injuries. To study astrogliosis, we developed a reproducible in vitro model in which low temperature injury to cultured astrocytes could be induced by placing the culture dish onto a copper pipe pre-cooled by liquid nitrogen. Using this model, the relationship between the temperature decline and the severity of cellular damage was analyzed. An increase in the expression of some known injury-related proteins, such as glial fibrillary acidic protein (GFAP), immediate early response genes (IEGs), and heat shock proteins 70 (HSP70), was demonstrated in astrocytes after low temperature trauma. With the use of this low temperature trauma model, the flexibility in the temperature control and injury area may allow researchers to evaluate cryotherapy and cryosurgery, which could be applicable to future development of quality health care.
AB - Astrogliosis is an inevitable and rapid response of astrocytes to physical, chemical and pathological injuries. To study astrogliosis, we developed a reproducible in vitro model in which low temperature injury to cultured astrocytes could be induced by placing the culture dish onto a copper pipe pre-cooled by liquid nitrogen. Using this model, the relationship between the temperature decline and the severity of cellular damage was analyzed. An increase in the expression of some known injury-related proteins, such as glial fibrillary acidic protein (GFAP), immediate early response genes (IEGs), and heat shock proteins 70 (HSP70), was demonstrated in astrocytes after low temperature trauma. With the use of this low temperature trauma model, the flexibility in the temperature control and injury area may allow researchers to evaluate cryotherapy and cryosurgery, which could be applicable to future development of quality health care.
KW - astrocytes
KW - glial fibrillary acidic protein (GFAP)
KW - gliosis
KW - heat shock protein
KW - immediate early gene
KW - injury model
KW - low temperature trauma
UR - http://www.scopus.com/inward/record.url?scp=10844278156&partnerID=8YFLogxK
UR - https://link.springer.com/article/10.1007/s11064-004-6891-z
U2 - 10.1007/s11064-004-6891-z
DO - 10.1007/s11064-004-6891-z
M3 - Journal article
C2 - 15662852
AN - SCOPUS:10844278156
SN - 0364-3190
VL - 29
SP - 2171
EP - 2176
JO - Neurochemical Research
JF - Neurochemical Research
IS - 11
ER -
