A microfluidic device using a green organic light emitting diode as an integrated excitation source

Bo Yao, Guoan Luo*, Liduo Wang, Yudi Gao, Gangtie Lei, Kangning REN, Lingxin Chen, Yiming Wang, Yan Hu, Yong Qiu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

83 Citations (Scopus)

Abstract

A simply fabricated microfluidic device using a green organic light emitting diode (OLED) and thin film interference filter as integrated excitation source is presented and applied to fluorescence detection of proteins. A layer-by-layer compact system consisting of glass/PDMS microchip, pinhole, excitation filter and OLED is designed and equipped with a coaxial optical fiber and for fluorescence detection a 300 μm thick excitation filter is employed for eliminating nearly 80% of the unwanted light emitted by OLEDs which has overlaped with the fluorescence spectrum of the dyes. The distance between OLED illuminant and microchannels is limited to ∼1 mm for sensitive detection. The achieved fluorescence signal of 300 μM Rhodamine 6G is about 13 times as high as that without the excitation filter and 3.5 times the result of a perpendicular detection structure. This system has been used for fluorescence detection of Rhodamine 6G, Alexa 532 and BSA conjugates in 4% linear polyacrymide (LPA) buffer (in 1 × TBE, pH 8.3) and 1.4 fmol and 35 fmol mass detection limits at 0.7 nl injection volume for Alexa and Rhodamine dye have been obtained, respectively.

Original languageEnglish
Pages (from-to)1041-1047
Number of pages7
JournalLab on a Chip
Volume5
Issue number10
DOIs
Publication statusPublished - Oct 2005

Scopus Subject Areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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