TY - GEN
T1 - A microcalorimetric method for studying the toxic action of iron species on Escherichia coli
AU - Wang, F.
AU - Yao, J.
AU - Wang, Y. X.
AU - Tian, L.
AU - Chen, H. L.
AU - Zhou, Y.
AU - Djak, A.
AU - Choi, Martin M F
AU - Stupar, J.
N1 - Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2007
Y1 - 2007
N2 - A microcalorimetric technique based on bacterial heat-output was explored to evaluate the toxic effect of iron species on Escherichia coli. Power-time curves of the growth metabolism of Escherichia coli and the effect of different iron species on it were studied using the TAM III multi-channel microcalorimetric system, isothermal mode (stagnant), at 37±0.1°C. The differences in shape of the power-time curves and the thermodynamic and kinetic characteristics of E. coli growth have been compared. The dynamic parameters, that is, growth rate constant (k) and inhibitory ratio (I ) have been calculated. The experimental results reveal that the sequence of antibiotic activity of the different iron species (three forms) on Escherichia coli growth is Fe(III) (Ferric citrate) greater than Fe(II) (Ferrous chloride) greater than Fe(III) (Ferric chloride).These results are important to further studies of the physiology and pharmacology of iron species as antibacterial agents.
AB - A microcalorimetric technique based on bacterial heat-output was explored to evaluate the toxic effect of iron species on Escherichia coli. Power-time curves of the growth metabolism of Escherichia coli and the effect of different iron species on it were studied using the TAM III multi-channel microcalorimetric system, isothermal mode (stagnant), at 37±0.1°C. The differences in shape of the power-time curves and the thermodynamic and kinetic characteristics of E. coli growth have been compared. The dynamic parameters, that is, growth rate constant (k) and inhibitory ratio (I ) have been calculated. The experimental results reveal that the sequence of antibiotic activity of the different iron species (three forms) on Escherichia coli growth is Fe(III) (Ferric citrate) greater than Fe(II) (Ferrous chloride) greater than Fe(III) (Ferric chloride).These results are important to further studies of the physiology and pharmacology of iron species as antibacterial agents.
UR - http://www.scopus.com/inward/record.url?scp=84863250930&partnerID=8YFLogxK
M3 - Conference proceeding
AN - SCOPUS:84863250930
SN - 9780415451383
T3 - Water-Rock Interaction - Proceedings of the 12th International Symposium on Water-Rock Interaction, WRI-12
SP - 1621
EP - 1624
BT - Water-Rock Interaction - Proceedings of the 12th International Symposium on Water-Rock Interaction, WRI-12
T2 - 12th International Symposium on Water-Rock Interaction, WRI-12
Y2 - 31 July 2007 through 4 August 2007
ER -