A microbial biosensing system for monitoring methane

Guangming Wen; Jun Zheng; Chungui Zhao; Shaomin Shuang; Chuan Dong; Martin M.F. Choi

doi:10.1016/j.enzmictec.2008.04.006

A microbial biosensing system for monitoring methane

Guangming Wen, Jun Zheng, Chungui Zhao, Shaomin Shuang, Chuan Dong^*, Martin M.F. Choi

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

25 Citations (Scopus)

Abstract

A microbial biosensing system for the measurement of methane has been developed using an immobilized mixed culture of Pseudomonas aeruginosa and Klebsiella sp. together with a dissolved oxygen (O₂) sensor. When methane was applied to the biosensing system, the dissolved O₂ content decreased until a steady-state was reached. The biosensing system response depends linearly on methane concentration between 1.0 and 5.0% (v/v) with a detection limit of 0.3% (v/v) (S/N = 3) and a 100-s response time. Phosphate buffer (pH 7.0, 25 mM) and room temperature (20-25 °C) were chosen as the optimum working conditions. Some parameters including pH, temperature, operational and storage stability were studied in detail for characterization of the biosensing system.

Original language	English
Pages (from-to)	257-261
Number of pages	5
Journal	Enzyme and Microbial Technology
Volume	43
Issue number	3
DOIs	https://doi.org/10.1016/j.enzmictec.2008.04.006
Publication status	Published - 5 Sept 2008

Scopus Subject Areas

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology

User-Defined Keywords

Dissolved oxygen sensor
Methane
Microbial biosensing

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10.1016/j.enzmictec.2008.04.006

Cite this

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title = "A microbial biosensing system for monitoring methane",

abstract = "A microbial biosensing system for the measurement of methane has been developed using an immobilized mixed culture of Pseudomonas aeruginosa and Klebsiella sp. together with a dissolved oxygen (O2) sensor. When methane was applied to the biosensing system, the dissolved O2 content decreased until a steady-state was reached. The biosensing system response depends linearly on methane concentration between 1.0 and 5.0% (v/v) with a detection limit of 0.3% (v/v) (S/N = 3) and a 100-s response time. Phosphate buffer (pH 7.0, 25 mM) and room temperature (20-25 °C) were chosen as the optimum working conditions. Some parameters including pH, temperature, operational and storage stability were studied in detail for characterization of the biosensing system.",

keywords = "Dissolved oxygen sensor, Methane, Microbial biosensing",

author = "Guangming Wen and Jun Zheng and Chungui Zhao and Shaomin Shuang and Chuan Dong and Choi, {Martin M.F.}",

note = "Funding Information: The work described in this article was supported by grants from the Key Project of National Natural Science Foundation of China (no. 50534100) and the Natural Science Foundation of International Cooperation.",

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T1 - A microbial biosensing system for monitoring methane

AU - Wen, Guangming

AU - Zheng, Jun

AU - Zhao, Chungui

AU - Shuang, Shaomin

AU - Dong, Chuan

AU - Choi, Martin M.F.

N1 - Funding Information: The work described in this article was supported by grants from the Key Project of National Natural Science Foundation of China (no. 50534100) and the Natural Science Foundation of International Cooperation.

PY - 2008/9/5

Y1 - 2008/9/5

N2 - A microbial biosensing system for the measurement of methane has been developed using an immobilized mixed culture of Pseudomonas aeruginosa and Klebsiella sp. together with a dissolved oxygen (O2) sensor. When methane was applied to the biosensing system, the dissolved O2 content decreased until a steady-state was reached. The biosensing system response depends linearly on methane concentration between 1.0 and 5.0% (v/v) with a detection limit of 0.3% (v/v) (S/N = 3) and a 100-s response time. Phosphate buffer (pH 7.0, 25 mM) and room temperature (20-25 °C) were chosen as the optimum working conditions. Some parameters including pH, temperature, operational and storage stability were studied in detail for characterization of the biosensing system.

AB - A microbial biosensing system for the measurement of methane has been developed using an immobilized mixed culture of Pseudomonas aeruginosa and Klebsiella sp. together with a dissolved oxygen (O2) sensor. When methane was applied to the biosensing system, the dissolved O2 content decreased until a steady-state was reached. The biosensing system response depends linearly on methane concentration between 1.0 and 5.0% (v/v) with a detection limit of 0.3% (v/v) (S/N = 3) and a 100-s response time. Phosphate buffer (pH 7.0, 25 mM) and room temperature (20-25 °C) were chosen as the optimum working conditions. Some parameters including pH, temperature, operational and storage stability were studied in detail for characterization of the biosensing system.

KW - Dissolved oxygen sensor

KW - Methane

KW - Microbial biosensing

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DO - 10.1016/j.enzmictec.2008.04.006

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EP - 261

JO - Enzyme and Microbial Technology

JF - Enzyme and Microbial Technology

IS - 3

ER -

A microbial biosensing system for monitoring methane

Abstract

Scopus Subject Areas

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