A microbial biosensing system for monitoring methane

Guangming Wen, Jun Zheng, Chungui Zhao, Shaomin Shuang, Chuan Dong*, Martin M F CHOI

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

A microbial biosensing system for the measurement of methane has been developed using an immobilized mixed culture of Pseudomonas aeruginosa and Klebsiella sp. together with a dissolved oxygen (O2) sensor. When methane was applied to the biosensing system, the dissolved O2 content decreased until a steady-state was reached. The biosensing system response depends linearly on methane concentration between 1.0 and 5.0% (v/v) with a detection limit of 0.3% (v/v) (S/N = 3) and a 100-s response time. Phosphate buffer (pH 7.0, 25 mM) and room temperature (20-25 °C) were chosen as the optimum working conditions. Some parameters including pH, temperature, operational and storage stability were studied in detail for characterization of the biosensing system.

Original languageEnglish
Pages (from-to)257-261
Number of pages5
JournalEnzyme and Microbial Technology
Volume43
Issue number3
DOIs
Publication statusPublished - 5 Sep 2008

Scopus Subject Areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

User-Defined Keywords

  • Dissolved oxygen sensor
  • Methane
  • Microbial biosensing

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