A Metabolite Array Technology for Precision Medicine

Guoxiang Xie*, Lu Wang, Tianlu Chen, Kejun Zhou, Zechuan Zhang, Jiufeng Li, Beicheng Sun, Yike Guo, Xiaoning Wang, Yixing Wang, Hua Zhang, Ping Liu, Jeremy K. Nicholson, Weihong Ge, Wei JIA

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The application of metabolomics in translational research suffers from several technological bottlenecks, such as data reproducibility issues and the lack of standardization of sample profiling procedures. Here, we report an automated high-throughput metabolite array technology that can rapidly and quantitatively determine 324 metabolites including fatty acids, amino acids, organic acids, carbohydrates, and bile acids. Metabolite identification and quantification is achieved using the Targeted Metabolome Batch Quantification (TMBQ) software, the first cross-vendor data processing pipeline. A test of this metabolite array was performed by analyzing serum samples from patients with chronic liver disease (N = 1234). With high detection efficiency and sensitivity in serum, urine, feces, cell lysates, and liver tissue samples and suitable for different mass spectrometry systems, this metabolite array technology holds great potential for biomarker discovery and high throughput clinical testing. Additionally, data generated from such standardized procedures can be used to generate a clinical metabolomics database suitable for precision medicine in next-generation healthcare.

Original languageEnglish
Pages (from-to)5709-5717
Number of pages9
JournalAnalytical Chemistry
Volume93
Issue number14
DOIs
Publication statusPublished - 13 Apr 2021

Scopus Subject Areas

  • Analytical Chemistry

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