A mediator-free bienzyme amperometric biosensor based on horseradish peroxidase and glucose oxidase immobilized on carbon nanotube modified electrode

Yan Li Yao; Kwok Keung Shiu

doi:10.1002/elan.200804297

A mediator-free bienzyme amperometric biosensor based on horseradish peroxidase and glucose oxidase immobilized on carbon nanotube modified electrode

Yan Li Yao^*, Kwok Keung Shiu

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

42 Citations (Scopus)

Abstract

Multiwalled carbon nanotube (CNT) modified glassy carbon electrode immobilized with horseradish peroxidase (HRP) in Nafion coating showed direct electron transfer between HRP enzyme and the CNT-modified electrode. A mediator-free bienzyme glucose biosensor based on horseradish peroxidase and glucose oxidase was constructed. The bienzyme biosensor exhibited a high sensitivity for glucose detection at zero applied potential.

Original language	English
Pages (from-to)	2090-2095
Number of pages	6
Journal	Electroanalysis
Volume	20
Issue number	19
DOIs	https://doi.org/10.1002/elan.200804297
Publication status	Published - Oct 2008

User-Defined Keywords

Bienzyme glucose biosensor
Carbon nanotubes
Direct electrochemistry
Glucose oxidase
Horseradish peroxidase
Mediator-free

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10.1002/elan.200804297

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abstract = "Multiwalled carbon nanotube (CNT) modified glassy carbon electrode immobilized with horseradish peroxidase (HRP) in Nafion coating showed direct electron transfer between HRP enzyme and the CNT-modified electrode. A mediator-free bienzyme glucose biosensor based on horseradish peroxidase and glucose oxidase was constructed. The bienzyme biosensor exhibited a high sensitivity for glucose detection at zero applied potential.",

keywords = "Bienzyme glucose biosensor, Carbon nanotubes, Direct electrochemistry, Glucose oxidase, Horseradish peroxidase, Mediator-free",

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AU - Shiu, Kwok Keung

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AB - Multiwalled carbon nanotube (CNT) modified glassy carbon electrode immobilized with horseradish peroxidase (HRP) in Nafion coating showed direct electron transfer between HRP enzyme and the CNT-modified electrode. A mediator-free bienzyme glucose biosensor based on horseradish peroxidase and glucose oxidase was constructed. The bienzyme biosensor exhibited a high sensitivity for glucose detection at zero applied potential.

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KW - Carbon nanotubes

KW - Direct electrochemistry

KW - Glucose oxidase

KW - Horseradish peroxidase

KW - Mediator-free

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A mediator-free bienzyme amperometric biosensor based on horseradish peroxidase and glucose oxidase immobilized on carbon nanotube modified electrode

Abstract

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