A mediator-free bienzyme amperometric biosensor based on horseradish peroxidase and glucose oxidase immobilized on carbon nanotube modified electrode

Yan Li Yao*, Kwok Keung SHIU

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

Multiwalled carbon nanotube (CNT) modified glassy carbon electrode immobilized with horseradish peroxidase (HRP) in Nafion coating showed direct electron transfer between HRP enzyme and the CNT-modified electrode. A mediator-free bienzyme glucose biosensor based on horseradish peroxidase and glucose oxidase was constructed. The bienzyme biosensor exhibited a high sensitivity for glucose detection at zero applied potential.

Original languageEnglish
Pages (from-to)2090-2095
Number of pages6
JournalElectroanalysis
Volume20
Issue number19
DOIs
Publication statusPublished - Oct 2008

Scopus Subject Areas

  • Analytical Chemistry
  • Electrochemistry

User-Defined Keywords

  • Bienzyme glucose biosensor
  • Carbon nanotubes
  • Direct electrochemistry
  • Glucose oxidase
  • Horseradish peroxidase
  • Mediator-free

Fingerprint

Dive into the research topics of 'A mediator-free bienzyme amperometric biosensor based on horseradish peroxidase and glucose oxidase immobilized on carbon nanotube modified electrode'. Together they form a unique fingerprint.

Cite this