A lymphatic route for a hyperbranched heteroglycan from Radix Astragali to trigger immune responses after oral dosing

Quanwei Zhang, Lifeng Li, Shuang Hao, Man Liu, Chuying Huo, Jianjun Wu, Hongbing Liu, Wanrong Bao, Hongming Zheng, Zhipeng Li, Huiyuan Cheng, Hauyee Fung, Tinlong Wong, Pingchung Leung, Shunchun Wang, Ting Li, Ge Zhang, Min Li, Zhongzhen Zhao, Wei JiaZhaoxiang Bian, Timothy Mitchison, Jingchao Zhang*, Aiping Lyu*, Quanbin Han*, Handong Sun

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

6 Citations (Scopus)

Abstract

Gut barrier makes a huge research gap between in vivo and in vitro studies of orally bioactive polysaccharides: whether/how they contact the related cells in vivo. A hyperbranched heteroglycan RAP from Radix Astragali, exerting antitumor and immunomodulatory effects in vitro and in vivo, is right an example. Here, we determined first that RAP's antitumor activity is immune-dependent. Being undegraded and non-absorbing, RAP quickly entered Peyer's patches (PPs) in 1 h where it directly targeted follicle dendritic cells and initiated antitumor immune responses. RAP was further delivered to mesenteric lymph node, bone marrow, and tumor. By contrast, the control Dendrobium officinale polysaccharide did not enter PPs. These findings revealed a blood/microbiota-independent and selective lymphatic route for orally administrated RAP to directly contact immune cells and trigger antitumor immune responses. This route bridges the research gap between the in vitro and in vivo studies and might apply to many other bioactive polysaccharides.

Original languageEnglish
Article number119653
Number of pages12
JournalCarbohydrate Polymers
Volume292
DOIs
Publication statusPublished - 15 Sept 2022

Scopus Subject Areas

  • Materials Chemistry
  • Polymers and Plastics
  • Organic Chemistry

User-Defined Keywords

  • Antitumor immune responses
  • Intact
  • Polysaccharide
  • Radix Astragali
  • Targeting route

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