A Luminescent Cocaine Detection Platform Using a Split G-Quadruplex-Selective Iridium(III) Complex and a Three-Way DNA Junction Architecture

Edmond Dik Lung MA*, Modi Wang, Bingyong He, Chao Yang, Wanhe Wang, Chung Hang Leung

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

In this study, a series of 10 in-house cyclometalated iridium(III) complexes bearing different auxiliary ligands were tested for their selectivity toward split G-quadruplex in order to construct a label-free switch-on cocaine detection platform employing a three-way junction architecture and a G-quadruplex motif as a signal output unit. Through two rounds of screening, we discovered that the iridium(III) complex 7 exhibited excellent selectivity toward the intermolecular G-quadruplex motif. A detection limit as low as 30 nM for cocaine can be achieved by this sensing approach with a linear relationship between luminescence intensity and cocaine concentration established from 30 to 300 nM. Furthermore, this sensing approach could detect cocaine in diluted oral fluid. We hope that our simple, signal-on, label-free oligonucleotide-based sensing method for cocaine using a three-way DNA junction architecture could act as a useful platform in bioanalytical research.

Original languageEnglish
Pages (from-to)19060-19067
Number of pages8
JournalACS applied materials & interfaces
Volume7
Issue number34
DOIs
Publication statusPublished - 18 Aug 2015

Scopus Subject Areas

  • Materials Science(all)

User-Defined Keywords

  • cocaine
  • G-quadruplex
  • iridium(III) complex
  • label-free
  • three-way junction

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