A luminescence switch-on probe for terminal deoxynucleotidyl transferase (TdT) activity detection by using an iridium(III)-based i-motif probe

Lihua Lu; Modi Wang; Li Juan Liu; Chun Yuen Wong; Chung Hang Leung; Edmond Dik Lung Ma

doi:10.1039/c5cc02790j

A luminescence switch-on probe for terminal deoxynucleotidyl transferase (TdT) activity detection by using an iridium(III)-based i-motif probe

Lihua Lu, Modi Wang, Li Juan Liu, Chun Yuen Wong, Chung Hang Leung^*, Edmond Dik Lung Ma^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

61 Citations (Scopus)

Abstract

An iridium(III) complex exhibiting higher responce towards i-motif DNA over dsDNA and ssDNA was employed for the construction of a TdT activity detection platform. The assay exhibited a linear signal enhancement for TdT in the concentration range of 0 to 8 U mL^-1, and the limit of detection for TdT was 0.25 U mL^-1.

Original language	English
Pages (from-to)	9953-9956
Number of pages	4
Journal	Chemical Communications
Volume	51
Issue number	49
DOIs	https://doi.org/10.1039/c5cc02790j
Publication status	Published - 21 Jun 2015

Scopus Subject Areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/c5cc02790j

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title = "A luminescence switch-on probe for terminal deoxynucleotidyl transferase (TdT) activity detection by using an iridium(III)-based i-motif probe",

abstract = "An iridium(III) complex exhibiting higher responce towards i-motif DNA over dsDNA and ssDNA was employed for the construction of a TdT activity detection platform. The assay exhibited a linear signal enhancement for TdT in the concentration range of 0 to 8 U mL-1, and the limit of detection for TdT was 0.25 U mL-1.",

author = "Lihua Lu and Modi Wang and Liu, {Li Juan} and Wong, {Chun Yuen} and Leung, {Chung Hang} and Ma, {Edmond Dik Lung}",

year = "2015",

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doi = "10.1039/c5cc02790j",

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volume = "51",

pages = "9953--9956",

journal = "Chemical Communications",

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publisher = "Royal Society of Chemistry",

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T1 - A luminescence switch-on probe for terminal deoxynucleotidyl transferase (TdT) activity detection by using an iridium(III)-based i-motif probe

AU - Lu, Lihua

AU - Wang, Modi

AU - Liu, Li Juan

AU - Wong, Chun Yuen

AU - Leung, Chung Hang

AU - Ma, Edmond Dik Lung

PY - 2015/6/21

Y1 - 2015/6/21

N2 - An iridium(III) complex exhibiting higher responce towards i-motif DNA over dsDNA and ssDNA was employed for the construction of a TdT activity detection platform. The assay exhibited a linear signal enhancement for TdT in the concentration range of 0 to 8 U mL-1, and the limit of detection for TdT was 0.25 U mL-1.

AB - An iridium(III) complex exhibiting higher responce towards i-motif DNA over dsDNA and ssDNA was employed for the construction of a TdT activity detection platform. The assay exhibited a linear signal enhancement for TdT in the concentration range of 0 to 8 U mL-1, and the limit of detection for TdT was 0.25 U mL-1.

UR - http://www.scopus.com/inward/record.url?scp=84931281905&partnerID=8YFLogxK

U2 - 10.1039/c5cc02790j

DO - 10.1039/c5cc02790j

M3 - Journal article

C2 - 25999030

AN - SCOPUS:84931281905

SN - 1359-7345

VL - 51

SP - 9953

EP - 9956

JO - Chemical Communications

JF - Chemical Communications

IS - 49

ER -

A luminescence switch-on probe for terminal deoxynucleotidyl transferase (TdT) activity detection by using an iridium(III)-based i-motif probe

Abstract

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